The volatile compounds of the beers adjusted for sensory profiling were analysed with a method adapted from one reported by Krogerus et al. [24 (link)]. Briefly, the headspace volatiles were first extracted by solid phase microextraction (SPME) with 2 cm 50/30 μm divinylbenzene/carboxen/ poly(dimethylsiloxane) (DVB/CAR/PDMS) fiber (Stableflex, 23 Ga, Autosampler) (Supelco, Bellafonte, PA) at 70 ℃ for 30 min. A DB-FFAP column (25 m × 200 µm × 0,33 µm) was used to separate the compounds on an Agilent 7890A + 5975C GC–MS instrument (Agilent Technologies, CA, US). Scan range of 25–500 amu was used. 3-octanol and 3,4-dimethylphenol were used as internal standards in isomolar amounts. The peak areas were normalized to internal standard peak areas.
Agilent 7890a 5975c gc ms instrument
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890A + 5975C GC–MS instrument is a gas chromatograph-mass spectrometer system designed for analytical applications. It combines the Agilent 7890A gas chromatograph and the Agilent 5975C mass spectrometer to provide separation, identification, and quantification of chemical compounds.
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2 protocols using agilent 7890a 5975c gc ms instrument
1
Headspace Volatiles Analysis of Beers
2
Characterizing Materials via Advanced Instrumentation
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The D8 advance X-ray diffractometer of Brooke spectroscopic instrument company, Germany, using a radiation source Cu (Kα = 1.54178 nm, 40 kV and 15 mA) with a scanning rate of 10 (°) min−1, continuous scanning mode, wide-angle scanning range is 5–90° and small-angle scanning range is 0.5–8.0°. Autosorb-IQ2-MP automatic physical static analyzer of Cantor instrument company, the liquid nitrogen temperature is 77 K. SU8010 field emission scanning electron microscope of Hitachi company, Japan, with accelerating voltage of 15 kV and working distance of WD = 4 mm. JEM-2100F high resolution transmission electron microscope of Japan Electronics Co., Ltd., accelerating voltage 200 kV. ESCALAB 250Xi photoelectron spectrometer of Thermo Scientific company, USA, uses monochromatic Al target as X-ray source. Agilent 7890a/5975c GC/MS instrument of Agilent Technology Co., Ltd. of the United States, the carrier gas is high-purity nitrogen, HP-5MS column.
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