Propoxur

Chemicals were purchased from Sigma Chemicals UK (Haverhill, UK) unless otherwise stated. Permethrin (lot no. SZBD142XV), DDT (lot no. 360129B) and malathion (lot no. 352-138A) (97–99% pure) were a gift from the Liverpool School of Tropical Medicine, UK. Propoxur (lot no. SZBD302XV) was purchased from Bayer (Leverkusen, Germany). UVmaxELx 800^TM microtiter plate reader was from Molecular Devices, Bio-Tek, Winooski, VT, USA. The protein assay kit was from Bio-Rad (Dalkeith, UK).

Propoxur (99%) was purchased from Bayer Company Limited. Methomyl (80%) was from Shandong Jining Chemical Plant. Azamethiphos (95%) was from Shanghai Yongyuan Chemical Group Company Limited. Beta-cypermethrin (95.2%) and DDVP (98.7%) were obtained from Tianjin Longdeng Chemical Company Limited. Bifenthrin (95%) and Deltamethrin (99%) were from Jiangsu Yangnong Chemical Company Group Company Limited. Leupeptin, phenylmethanesulfonyl fluoride (PMSF), eserine hemisulfate, procainamide hydrochloride, bovine serum albumin (BSA), and acetylthiocholine iodide (ATChI) were acquired from Sigma Chemical Company. Tetraethylammonium iodide (Net₄I) was from Sigma-Aldrich Company Limited. EDTA-Na₂ was from BIO-RAD Laboratories. 2-Ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline (EEDQ) and 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB) were from Fluka Chemical Company. ECH-sepharose 4B was from Beijing Solarbio Science & Technology Company Limited. Sephadex G-25 columns were purchased from Amersham Pharmacia Biotech Incorporated. Other chemicals were purchased from commercial suppliers, all were analytical quality.