DOX, DIL and Trypan blue powder were purchased from Sigma Aldrich Co. (Saint Louis, Missouri, USA) while Verapamil was purchased from Abbott. Fetal bovine serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM), Trypsin–EDTA (0.05%) and phosphate buffer saline (PBS, pH 7.4) were purchased from Thermo Fisher Scientific Inc (USA). Acridine orange (AO) (Molecular Probes, Eugene, OR, cat. no. A1301) and Giemsa stain (GS-10), rhodamine 123 (R8004 SIGMA) have been also supplied. The cell cycle determination kit was purchased from Cayman Chemical Company (USA). Animal kits include Rat CK-MB (Catalog No: E-EL-R1327 (USA)), MDA (Catalog No: E-EL-0060 (USA)), TAC (Catalogue No. 201-11-1187 (USA)) and GPx (Catalog No: CAT. No. GP 2524 (Biodiagnostic, Dokki, Egypt).
Cell cycle determination kit
Manufactured by Cayman Chemical
Sourced in United States
The Cell Cycle Determination Kit is a laboratory tool designed to analyze the stages of the cell cycle. It provides the essential components for determining the distribution of cells within the different phases of the cell cycle, such as G0/G1, S, and G2/M phases.
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Lab products found in correlation
2 protocols using cell cycle determination kit
1
Cardiac Injury Biomarker Evaluation
2
Cytotoxicity Assay for HCT-116 CRC Cells
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Drugs and chemicals. The CIS "Ebewe" and RSVL were purchased from Sigma-Aldrich (St Louis, MO, USA). The CIS was stored at room temperature (not exceeding 25˚C), protected from light and diluted in phosphate-buffered saline (PBS). RSVL was supplied as a white powder, with a yellow cast and stored at -20˚C. Media and other tissue culture reagents used in the present study were all purchased Sigma-Aldrich. The cell cycle determination kit was purchased from Cayman Chemical Company (Ann Arbor, MI, USA).
Cells and cell cultures. The HCT-116 human CRC cells were acquired from Professor Abdel-Moneim M. Osman, from the National Cancer Institute, Cairo University (Egypt). The cells were grown as monolayer cultures and maintained in RPMI-1640 medium, supplemented with penicillin G and streptomycin antibiotics and 10% fetal bovine serum (FBS), and were cultured at 37˚C in a humidified incubator containing 5% CO 2 . The cells were collected by trypsinization and passaged every 4-6 days. The passage level was in the range of 5-10, following receipt of the cell line from the source.
Cells and cell cultures. The HCT-116 human CRC cells were acquired from Professor Abdel-Moneim M. Osman, from the National Cancer Institute, Cairo University (Egypt). The cells were grown as monolayer cultures and maintained in RPMI-1640 medium, supplemented with penicillin G and streptomycin antibiotics and 10% fetal bovine serum (FBS), and were cultured at 37˚C in a humidified incubator containing 5% CO 2 . The cells were collected by trypsinization and passaged every 4-6 days. The passage level was in the range of 5-10, following receipt of the cell line from the source.
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