Odysseyh scanner and software

Manufactured by LI COR

Sourced in United States

The LI-COR Odyssey H scanner is a high-resolution imaging system designed for protein and nucleic acid detection and quantification. The scanner utilizes near-infrared fluorescence technology to capture and analyze images of protein and DNA gels, Western blots, and microarrays. The accompanying Odyssey software provides data analysis and image processing capabilities to support the scanner's functionality.

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Lab products found in correlation

Odyssey blocking buffer, by LI COR (2 mentions) Goat anti rabbit igg h l 800 cw, by LI COR (1 mentions) Goat anti rabbit 680rd, by LI COR (1 mentions) Gapdh, by Bioss Antibodies (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

4 protocols using odysseyh scanner and software

Western Blot Analysis of Cellular Signaling

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Protein extracts were prepared with RIPA buffer containing a mixture of protease inhibitors as described.^{23 (link)} Briefly, 50 μg of protein was applied to a 12% SDS/PAGE and transferred to nitrocellulose membranes. Membranes were incubated with Odyssey blocking buffer (Li-Cor) prior to incubation with polyclonal or monoclonal antibodies to phospho-JNK, p53, caspase3, or β-actin overnight at 4°C. Goat anti-rabbit IgG (HþL) 800 CW and/or goat anti-mouse (HþL) was applied for 60 minutes at room temperature (1:25,000, LI-COR) prior to washing with 1X Phosphate Buffered Saline Tween-20 (PBS-T). Visualization and quantification were carried out with the LI-COR OdysseyH scanner and software (LI-COR Biosciences).

O’Connor N.A., Einbond L.S., Redenti S., Sauane M, & Jitianu A. (2018). A Self-degradable Curcumin Polymer with Anti-cancer Activity. Journal of applied polymer science, 135(47), 46867.

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Gel Shift Assay for Transcription Factor Binding

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Nuclear extracts were prepared as previous described.²⁴ Biotin‐labeled and corresponding unlabeled oligonucleotides containing the various genotypes were synthesized by BGI Genomics. Equal amounts of complementary oligonucleotides were heated at 95°C for 5 minutes and annealed by stepwise reducing the temperature to 25°C in 1 hours. EMSA was performed by using the commercial LightShift^® Chemiluminescent EMSA kit (Pierce). Membrane was labeled with IRDye 800CW streptavidin and then detected by LI‐COR OdysseyH scanner and software (LI‐COR Biosciences).

Sun C., Sun D., Luan Z., Dai X., Bie X., Ming W., Sun X., Huo X.X., Lu T, & Zhang D. (2020). Association of SOX11 Polymorphisms in distal 3′UTR with Susceptibility for Schizophrenia. Journal of Clinical Laboratory Analysis, 34(8), e23306.

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Western Blot Analysis of Protein Targets

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Protein extracts were prepared with RIPA buffer containing a mixture of protease inhibitors as described (19 (link)). Briefly, fifty micrograms of protein were applied to a 12% SDS/PAGE and transferred to nitrocellulose membranes. Membranes were incubated with Odyssey blocking buffer (Li-Cor) prior to incubation with polyclonal or monoclonal antibodies to phospho-eIF2α, total eIF2α, CHOP, cyclin D1, cyclin E, survivin, Bcl2, α-tubulin, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), Hsp27, c-jun, and β-actin overnight at 4°C. Goat anti-rabbit IgG (H+L) 800 CW, goat anti-rabbit (680 RD) and/or goat anti-mouse (H+L) was applied for 60 minutes at room temperature (1:25000, LI-COR) prior to washing with 1× Phosphate Buffered Saline Tween-20 (PBS-T). Visualization and quantification was carried out with the LI-COR OdysseyH scanner and software (LI-COR Biosciences).

Persaud L., Zhong X., Alvarado G., Do W., Dejoie J., Zybtseva A., Aktas B.H, & Sauane M. (2017). eIF2α Phosphorylation Mediates IL24-induced Apoptosis through Inhibition of Translation. Molecular cancer research : MCR, 15(8), 1117-1124.

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Western Blot Analysis of MRP1, EGR1, and GAPDH

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The indicated cells were lysed in precooled lysis buffer (Western and IP Cell Lysate Kit from Beyotime Biotechnology, China) containing 150 mM NaCl, 20 mM Tris-Cl (pH 7.5), 1% Triton X-100, and various inhibitors such as EDTA, sodium pyrophosphate, β-glycerophosphate, sodium orthovanadate, and leupeptin. The protein lysates were separated on 10 % SDS-PAGE gels and electrophoretically transferred to polyvinylidene di uoride (PVDF) membranes (Merck Millipore, Germany). After antigen blocking, the membranes were incubated with primary antibodies (antimouse IgG Bioss) overnight at 4 °C with gentle shaking. Membranes were probed with the following primary antibodies: MRP1 (1:300, Bioss, China), EGR1 (1:300, Bioss, China), and GAPDH (internal control, 1:300, Bioss, China). The secondary rabbit anti-mouse IgG (LI-COR Biotechnology, USA) antibody was added subsequently at a 1:1000 dilution and incubated at 4 °C for 1 hour with gentle shaking [29] . Bands were visualized with the LI-COR OdysseyH scanner and software (LI-COR Biotechnology, USA). Blots (and gels) were imaged using an Odyssey Infrared Imaging System Scan resolution.

Shao S., Du Y., Zhu S., Zhang W., Zhang Z., Sun Y., Cui T., Liu X., Feng Y., & Liu C. (2020). Effect of EGR1-Mediated LncRNA HOTAIR on MRP1 Gene Expression and MDR in Lung Cancer.

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