Lauric (dodecanoic) acid (d0, d3, and d23) and
12-OH lauric acid were purchased from Sigma-Aldrich
and used without further purification. Lauric acid labeled with only
one isotopic atom (d1 or t1) was prepared by the reaction of 12-bromododecanoic
acid with NaB2H4 or NaB3H 4, respectively, in (CH3)2SO.28 The d1 (2H) reaction
was conducted on a 10 mmol scale and the t1 (3H) reaction on a 0.10 mmol scale (with 5 mCi of NaB3H4, American Radiolabeled Chemicals, St. Louis,
MO), and the product was recrystallized from a C2H5OH/H2O mixture. The yields for the two reactions
were 78 and 87%, respectively; the atomic excess of d1-lauric acid was 90%, and the specific radioactivity
of t1-lauric acid was 0.23 mCi/mmol. [1-14C]Lauric acid was purchased from American Radiolabeled Chemicals.
Other chemicals were of the highest commercially available grade.
12-OH lauric acid were purchased from Sigma-Aldrich
and used without further purification. Lauric acid labeled with only
one isotopic atom (d1 or t1) was prepared by the reaction of 12-bromododecanoic
acid with NaB2H4 or NaB3H 4, respectively, in (CH3)2SO.28 The d1 (2H) reaction
was conducted on a 10 mmol scale and the t1 (3H) reaction on a 0.10 mmol scale (with 5 mCi of NaB3H4, American Radiolabeled Chemicals, St. Louis,
MO), and the product was recrystallized from a C2H5OH/H2O mixture. The yields for the two reactions
were 78 and 87%, respectively; the atomic excess of d1-lauric acid was 90%, and the specific radioactivity
of t1-lauric acid was 0.23 mCi/mmol. [1-14C]Lauric acid was purchased from American Radiolabeled Chemicals.
Other chemicals were of the highest commercially available grade.