Brain endothelial cells obtained from rats subjected to embolic stroke were used for Western blot and quantitative real-time polymerase chain reaction as previously described (23 (link)). Antibodies to occludin (BD Bioscience, San Diego, CA), claudin-5 (BD Bioscience, San Diego, CA), tPA (Molecular Innovations, Novi, MI), PAI-1 (Molecular Innovations, Novi, MI) or actin (Santa Cruz, Santa Cruz, CA) were used. The primers and probes for real-time PCR were: tPA Forward: 5’ – CCCTGA CCCCGACGTACAG-3’, Reverse: 5’ – ATGCTT GCCGTAGCCAGAA-3’ Probe: FAM – TCCCTGA CTGGACAGAGTGTGAGC-TAMRA; β-actin Forward: 5’-TTCAACACCCCAGCCATGT -3’, Reverse: 5’-TGGTACGACCAGAGGCATACAG -3’, Probe: FAM – CGTAGCCATCCAG GCTGTGTTG -TAMRA. RT2 Profiler PCR array system (PARN-015A, SABiosciences, Frederick, MD) profiling 84 genes related to endothelial cell biology and 6 housekeeping genes were used for PCR array study. Ct values of each gene were calculated and imported into PCR Array Data Analysis Portal (SABiosciences, Frederick, MD).
Claudin 5
Manufactured by BD
Claudin-5 is a protein that is a critical component of tight junctions in endothelial cells. It plays a key role in the regulation of the blood-brain barrier permeability. This product can be used for research purposes to study the structure and function of tight junctions and the blood-brain barrier.
Automatically generated - may contain errors
Lab products found in correlation
Occludin, by BD (1 mentions)
Pai 1, by Innovative Research (1 mentions)
Paxillin, by BD (1 mentions)
Py419 src, by Cell Signaling Technology (1 mentions)
Py118 paxillin, by Abcam (1 mentions)
Y 27632, by Merck Group (1 mentions)
Fitc bsa, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Sb203580, by Merck Group (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Doxycycline, by MP Biomedicals (1 mentions)
Hsp27, by Cell Signaling Technology (1 mentions)
Ve cadherin, by Santa Cruz Biotechnology (1 mentions)
Pt180 y182 p38, by Cell Signaling Technology (1 mentions)
Bay11 7082, by Selleck Chemicals (1 mentions)
2 protocols using claudin 5
1
Stroke-Induced Endothelial Cell Analysis
2
Endothelial Barrier Permeability Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TNF-α was obtained from R&D Systems. Doxycycline was from MP Biomedicals. SB203580 was from Millipore. BAY 11–7082 was from Selleck Chemicals. Y-27632, BSA, and FITC-BSA were from Sigma-Aldrich. The following antibodies were used for Western blot and/or immunofluorescence: pY419 Src (#2101), pS82 HSP27 (#2401), HSP27 (#2402) and pT180Y182 p38 (#4511) were obtained from Cell Signaling; pY118 paxillin (#ab75740) from Abcam; Src (GD11) and anti-pY (4G10) from Millipore; Csk (# sc-286), FAK (#sc-557), p38 (#sc-535) and VE-cadherin (#sc-6458) from Santa Cruz Biotechnology; paxillin (#612405) from BD Biosciences; claudin 5 (#34–1600), pY773 ITGB3 (#44-876G), pY397 FAK (#44-624G) and pY861 FAK (#44-626G) from Life Technologies; pS15 HSP27 (#2231–1) from Epitomics. Anti pT18S19 MLC was described previously [32 (link)].
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