Dulbecco's modified Eagle's medium (DMEM), Roswell Park Memorial Institute (RPMI) medium, fetal bovine serum (FBS) and penicillin/streptomycin (P/S) were purchased from Welgene Inc. WST-1 and bovine serum albumin (BSA) were purchased from Sigma-Aldrich; Merck KGaA. A TUNEL assay kit was obtained from Promega Corporation. Hoechst dye, Mito-tracker™ Red CMXRos and the Alexa-Fluor 488-conjugated donkey anti-Rabbit antibody were purchased from Thermo Fisher Scientific, Inc. The anti-Ki-67 antibody was purchased from Abcam (cat. no. ab15580). The aspirin conjugated DK143 [(E)-2-(4-(2,3-dimethoxyphenyl)acryloyl)-4-methoxyphenyl-2-acetoxybenzoate, AS-DK143] and AS-DK143-loaded methoxy poly(ethylene glycol)-poly(L-lactide) NPs (AS-DK143-NPs) were prepared and characterized according to our previous report (22 (link)). HeLa cell line, HepG2, which is a hepatoblastoma cell line, and AGS cell line were purchased from the Korean Cell Line Bank; Korean Cell Line Research Foundation. BXPC-3 cells were obtained from the Japanese Collection of Research Bioresources Cell Bank.
Bxpc 3 cells
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
BXPC-3 cells are a human pancreatic adenocarcinoma cell line derived from a primary tumor. They are commonly used in research to study pancreatic cancer.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 conjugated donkey anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Welgene (1 mentions)
Anti ki67 antibody, by Abcam (1 mentions)
Tunel assay kit, by Promega (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Hoechst dye, by Thermo Fisher Scientific (1 mentions)
Wst 1, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Hela cell line, by Korean Cell Line Bank (1 mentions)
Ags cell line, by Korean Cell Line Bank (1 mentions)
Hepg2, by Korean Cell Line Bank (1 mentions)
Dulbecco s modified eagle s medium, by Welgene (1 mentions)
Hpastec, by ScienCell (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by ScienCell (1 mentions)
Horse serum, by American Type Culture Collection (1 mentions)
Stellate cell growth supplement, by ScienCell (1 mentions)
Penicillin streptomycin, by ScienCell (1 mentions)
2 protocols using bxpc 3 cells
1
Aspirin-conjugated NPs for anti-cancer effects
2
Isolation and Culture of Pancreatic Cell Lines
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HPaSteCs were purchased from ScienCell Research Laboratories (San Diego, CA, USA) and cultured in stellate cell medium (ScienCell Research Laboratories) supplemented with 2% fetal bovine serum (ScienCell Research Laboratories), 1% stellate cell growth supplement (ScienCell Research Laboratories), and 1% penicillin/streptomycin (ScienCell Research Laboratories) in a humidified atmosphere at 37 °C with 5% CO2.
MIA PaCa-2 and PANC-1 cells were acquired from American Tissue Cell Culture (ATCC; Manassas, VA, USA), and OCUP-A2 is a PC cell line established by our group from a patient with malignant pancreatic neoplasm and liver metastasis [78 (link)]. These cell lines were grown in Dulbecco’s Modified Eagle’s Medium (Gibco, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) and 2.5% horse serum (ATCC; for MIA PaCa-2 cells only). BxPC-3 cells were acquired from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan) and cultured in RPMI 1640 medium with 10% heat-inactivated FBS (Gibco) in a humidified atmosphere at 37 °C with 5% CO2. Details of cell-based assays are provided in theSupplemental Materials .
MIA PaCa-2 and PANC-1 cells were acquired from American Tissue Cell Culture (ATCC; Manassas, VA, USA), and OCUP-A2 is a PC cell line established by our group from a patient with malignant pancreatic neoplasm and liver metastasis [78 (link)]. These cell lines were grown in Dulbecco’s Modified Eagle’s Medium (Gibco, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (Gibco) and 2.5% horse serum (ATCC; for MIA PaCa-2 cells only). BxPC-3 cells were acquired from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan) and cultured in RPMI 1640 medium with 10% heat-inactivated FBS (Gibco) in a humidified atmosphere at 37 °C with 5% CO2. Details of cell-based assays are provided in the
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