Et1701 80

Manufactured by Huabio

Sourced in China, United States

The ET1701-80 is a laboratory equipment model. It is designed for use in scientific and research settings. The device's core function is to perform specific laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and purely factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Ab196436, by Abcam (1 mentions) Ab215191, by Proteintech (1 mentions) L7543, by Merck Group (1 mentions) Ab209845, by Abcam (1 mentions) Bioruptor plus, by Diagenode (1 mentions) Ab49314, by Abcam (1 mentions) Ab6050, by Abcam (1 mentions) Hrp conjugated secondary antibody, by Bioworld Technology (1 mentions) Ha 51064 2 ap, by Proteintech (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Ab53715, by Abcam (1 mentions) P21 sc 6246, by Santa Cruz Biotechnology (1 mentions) Bca protein assay kit, by Meilun (1 mentions) Et1601 4, by Huabio (1 mentions) Ripa extraction buffer, by Solarbio (1 mentions) Ecl kit, by Fdbio (1 mentions) Et1602 47, by Huabio (1 mentions) Et1603 34, by Huabio (1 mentions) Pvdf membrane, by Merck Group (1 mentions)

4 protocols using et1701 80

Western Blot Analysis of Cell Death Regulators

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Fresh isolated c-Kit⁺ cells were lysed with NETN buffer (0.5% Nonidet P-40, 1 mM EDTA, 20 mM Tris–HCl, pH 8.0, 100 mM NaCl, containing protease and phosphatase inhibitors cocktail) for 30 min on ice, then centrifuged at 13,000 rcf for 10 min, 4°C. The supernatant was collected and boiled with 1 × SDS loading (4% SDS, 50 mM Tris base pH6.8, 20% glycerol, 04% Bromphenolblau) at 100°C for 8 min. In vitro cultured c-Kit⁺ cells, LSKs and HSCs were collected and sonicated by Sonicator (Diagenode, Bioruptor plus) in 1 × SDS loading and then boiled before running the SDS-PSGE according to the regular procedure for western blot. Samples were subjected to 13.5% or 15% SDS-PAGE with rabbit-derived primary antibodies against Caspase-3 (1:800, CST, 9662S) and LC3 (1:3000, Sigma, L7543), 10% SDS-PAGE with antibodies against RIPK3 (1:1000, PROSCI, 2283), MLKL (1:1000, ANGENT, AP14272b), Phospho-MLKL (1:1000, Abcom, ab196436), GSDMD (1:1000, Abcam, ab209845), GSDME (1:1000, Abcam, ab215191) and BECN1 (1:700, Proteintech, 11306–1-AP). H4 (1:1000, Proteintech, 16047–1-AP) and Actin (1:1000, HuaBio, ET1701–80) were used as the internal reference. HRP-linked anti-rabbit IgG (1:10,000, Cell Signalling, 7074S) was used as the secondary antibody.

Yang X., Ge L, & Wang J. (2020). BECN1 modulates hematopoietic stem cells by targeting Caspase-3-GSDME-mediated pyroptosis. Blood Science, 2(3), 89-99.

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Western Blot Analysis of Hedgehog Pathway Proteins

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Nuclear and cytoplasmic proteins were extracted with Nuclear and Cytoplasmic Protein Extraction Kit and quantified with a BCA Protein Assay Kit (Beyotime Institute of Biotechnology, Beijing, China) according to the manufacturers’ instructions. PBMCs and total cell lysates were prepared in 1 × SDS buffer, directly analyzed by SDS-PAGE, and transferred onto nitrocellulose membranes. After blocking in 5% BSA in Tris-buffered saline containing 0.1% Tween-20, the membranes were incubated with antibodies against Flag (F18804, Sigma, Inc.), HA (51064-2-AP, Proteintech), GLI3 (ab6050, Abcam), GLI1 (ab49314, Abcam), PTCH1 (ab53715, Abcam), SHH (ab32281, Abcam), GAPDH (ET1702-66, Huabio, Hangzhou, China), and β-actin (ET1701-80, Huabio). The antigen–antibody complexes were incubated with HRP-conjugated secondary antibodies (Bioworld, Nanjing, China) and visualized by an infrared imaging technique (Bio-Rad Laboratories, Inc.). The intensity of protein bands was quantified using ImageJ² to calculate the ratios of IntDen (GLI3)/IntDen (β-Actin) to ensure that the detection of protein bands was linearized. The Student’s t-test was used for the statistical comparison of GLI3 protein relative quantification between patients and normal controls.

Xiang Y., Li X., Zhan Z., Feng J., Cai H., Li Y., Fu Q., Xu Y., Jiang H, & Zhang X. (2020). A Novel Nonsense GLI3 Variant Is Associated With Polydactyly and Syndactyly in a Family by Blocking the Sonic Hedgehog Signaling Pathway. Frontiers in Genetics, 11, 542004.

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Western Blot Analysis of Stem Cell Markers

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Tissue was weighed and homogenized in RIPA extraction buffer (Solarbio, China). The homogenate was centrifuged at 4 °C for 15 min at 15,000 × g, then the supernatant was collected. The protein concentration was quantified with BCA protein assay kits (Meilun, China) according to the manufacturer’s instructions. Proteins were separated by 10% SDS polyacrylamide gel and then transferred onto PVDF membranes. The membranes were blocked with 5% skimmed milk for 1 h and then immunoblotted with primary antibodies: p53 (ab167161, Abcam,1:1000), p21 (sc-6246,Santa Cruz,1:500), Lgr5 (Bioss, bs-20747R, 1:1000), c-Myc (CST, #5605S, 1:1000), Cyclin D1 (CST, # 2978, 1:1000), Active β-Catenin (CST. # 8814, 1:1000), Reg4 (Abclonal, A13129, 1:1000), β-actin (ET1701-80, HUABIO, 1:1000), Gapdh (ET1601-4, HUABIO,1:1000) at 4 °C overnight. Membranes were then incubated with second antibodies (HA1019, HA1006, HUABIO,1:10,000) labeled with HRP at room temperature for 2 h, and bands were visualized using an ECL kit (Fdbio science, China). β-Actin or Gapdh was used as a reference protein.

Qi Y., He J., Zhang Y., Ge Q., Wang Q., Chen L., Xu J., Wang L., Chen X., Jia D., Lin Y., Xu C., Zhang Y., Hou T., Si J., Chen S, & Wang L. (2023). Heat-inactivated Bifidobacterium adolescentis ameliorates colon senescence through Paneth-like-cell-mediated stem cell activation. Nature Communications, 14, 6121.

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Protein Expression Analysis in Gastric Cancer

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Total protein was extracted from HGC-27 and HGC-27/DDPR GC cells after RIPA (Boster, China, AR0102) lysis. A BCA assay kit was used to determine the protein concentration. Twenty micrograms of protein were denatured, separated by electrophoresis, and then transferred to PVDF membranes (Millipore, USA). The membranes were blocked at room temperature for 1 h with 5% skim milk and then mixed with primary antibodies overnight at 4 °C. The secondary antibodies, goat anti-rabbit or mouse IgG-HRP, were coincubated at room temperature for 1 hour the next day. Protein strips were developed and photographed by exposure using a chemiluminescence substrate kit (Boster, China) and a UVP gel UV imager (ChemiDoc-It Imaging System, USA). The optical density values of each strip were quantified by ImageJ software.
Primary antibodies against LTBP2 (sc-166199, Santa Cruz, USA), P-gp (ET1611-30, Huabio, China), Bax (ET1603-34, Huabio), Bcl-2 (ET1610-20, Huabio), cleaved caspase-3 (ET1602-47, Huabio), cyclin D1 (ET1601-31, Huabio), Bcl-xL (ET1603-28, Huabio), Bcl-3 (ab259832, Abcam, USA), NFκB2 (EM1901-78, Huabio) and β-actin (ET1701-80, Huabio)

Wang J., Liang W., Wang X., Chen Z, & Jiang L. (2024). LTBP2 regulates cisplatin resistance in GC cells via activation of the NF-κB2/BCL3 pathway. Genetics and Molecular Biology, 47(2), e20230231.

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