Alpha tubulin

Manufactured by LI COR

Alpha tubulin is a key structural protein found in the cytoskeleton of eukaryotic cells. It plays a crucial role in the formation and function of microtubules, which are essential components of the cell's internal scaffold.

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Lab products found in correlation

Triton x 100, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Santa Cruz Biotechnology (1 mentions) Acetylated alpha tubulin, by Cell Signaling Technology (1 mentions) Phospho γh2a x, by Abcam (1 mentions) β tubulin, by Cell Signaling Technology (1 mentions)

2 protocols using alpha tubulin

PSMA6 Knockdown and Tubulin Analysis

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alpha tubulin and PSMA6 antibodies were purchased from Cell Signaling Technologies (Boston, MA). Briefly, PANC-1 cells were treated with 25 nM siControl (non-targeting) or siPSMA6 for 72 h, lysed with RIPA buffer, and supernatant was collected for gel electrophoresis. PVDF membrane was probed with alpha tubulin and PSMA6 antibodies and imaged on a Li-Cor FC and bands were analyzed with Li-Cor Odyssey software.

Bakke J., Wright W.C., Zamora A.E., Oladimeji P., Crawford J.C., Brewer C.T., Autry R.J., Evans W.E., Thomas P.G, & Chen T. (2019). Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cells. BMC Cancer, 19, 253.

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Multiparameter Immunofluorescence Staining Protocol

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Immunofluorescence staining was performed according to standard protocols as described previously (Al-Khadairi et al., 2019) . Briefly, 80,000 cells were plated on Poly-Lysine coated glass coverslips (Corning), fixed with 4% paraformaldehyde (ChemCruz) and permeabilized with 0.1% Triton X-100 (Sigma). Primary antibodies against β-tubulin (Cell Signaling), phospho-γH2AX (Abcam), alpha-tubulin (Li-Cor), acetylated alpha-tubulin (Cell Signaling), MAP1B

Naik A., & Decock J. (2021). Targeting LDHC dysregulates the cell cycle and improves sensitivity to cisplatin and olaparib.

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