Cell lysates were obtained using RIPA buffer (10 mM Tris, pH 7.2, 150 mM NaCl, 1.0% Triton X-100, 0.1% SDS, 5 mM EDTA, pH 8.0) with a protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN, USA). Bradford assay was used to evaluate protein concentration in each sample. Proteins (25 μg) were analysed with SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA). Then, 5% skim milk was used to block the membrane, which was then incubated overnight with the primary antibody. Rabbit anti-phospho-NF-κB (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-ACE2 (Cell Signaling), mouse anti-TMPRSS2 (Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-β-actin (Santa Cruz Biotechnology, Dallas, TX, USA) and rabbit anti-GAPDH (Cell Signaling Technology, Danvers, MA, USA) were used as primary antibodies. Horseradish peroxidase-conjugated antibodies anti-mouse or anti-rabbit (The Jackson Laboratory, Bar Harbor, ME, USA) were used as secondary antibodies. Protein bands were visualised by using the Clarity ECL chemiluminescence substrate (Bio-Rad) with Uvitec Imager (UVItec, Cambridge, UK) and then quantified using ImageJ software 4.1.
Rabbit anti ace2
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-ACE2 is a primary antibody that binds to the angiotensin-converting enzyme 2 (ACE2) protein. ACE2 is a cell surface receptor that acts as the entry point for the SARS-CoV-2 virus. This antibody can be used to detect and study the expression and localization of ACE2 in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti tmprss2, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti phospho nf κb, by Cell Signaling Technology (1 mentions)
Imager, by Uvitec (1 mentions)
Clarity ecl chemiluminescence substrate, by Bio-Rad (1 mentions)
Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
Bolt 4 12 bis tris plus gel, by Thermo Fisher Scientific (1 mentions)
2 protocols using rabbit anti ace2
1
Quantitative Western Blot Analysis
2
Western Blot Analysis of IFITM Proteins
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Whole‐cell extracts were prepared as previously described (Kajaste‐Rudnitski et al, 2006 (link), 2011 (link)). Samples were subjected to SDS–PAGE using Bolt 4–12% Bis‐Tris Plus Gels (Thermo Fisher Scientific), transferred to PVDF membrane by electroblotting, and blotted with rabbit anti‐IFITM3 polyclonal Ab (1:1,000 dilution, proteintech Cat# 11714‐1‐AP); rabbit anti‐IFITM2 polyclonal Ab (1:2,500, proteintech Cat# 12769‐1‐AP); mouse anti‐IFITM1 monoclonal Ab (1:5,000, proteintech Cat# 60074‐1‐Ig), rabbit anti‐ACE2 (1:1,000, Cell Signaling Technology 4355S), rabbit anti‐HA tag antibody—ChIP Grade (1:10,000 Abcam Cat# ab9110), mouse anti‐his (1:10,000 Millipore Cat# 05‐909). A mouse monoclonal anti‐beta‐actin Ab (1:10,000 dilution, Sigma‐Aldrich Cat# A2228) was used as a normalizer. After the incubation with primary antibodies, PVDF membranes were washed three times with tris‐buffered saline (TBS) 0.1% tween 20 for 5 min and then incubated for 1 h with rabbit or mouse IgG secondary antibodies (1:10,000; GE Healthcare Cat# NA934, GE Healthcare Cat# NA931).
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