Sterile tube

Manufactured by Greiner

Sourced in Austria

The Sterile Tube is a laboratory equipment designed for the safe and secure storage and transport of samples. It is a sterile, leak-proof container made of durable materials to protect the contents from contamination.

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Lab products found in correlation

Sterile tubes, by Sarstedt (2 mentions)

Close spelling variants of this product

Sterile Vacuette tubes Sterile polypropylene centrifuge tubes

4 protocols using sterile tube

Lymphocyte Cytotoxicity Assay Protocol

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After each elution period, 3 mL of the eluate was loaded into a new sterile tube (Greiner, Frickenhausen, Germany) and 100 µL of lymphocyte isolate containing 100 cells/µL was introduced. Lymphocytes were treated for 4 hours at 37 °C according to the OECD testing guideline (OECD, 1997). Negative control culture of lymphocytes was exposed only to RPMI 1640 medium for 4 hours.
After the treatment period, cultures were centrifuged for 3 min at 1000 rpm, the supernatant was discarded, and cells were transferred into a sterile tube. They were resuspended in 3 mL of RPMI 1640 medium. The process of lymphocyte centrifugation and washing with fresh medium was repeated twice. After second centrifugation, cells were resuspended and 10 µL of suspension was transferred to microscopic glass (Tlos, Zagreb, Croatia) for cytotoxicity testing.

Brzović Rajić V., Želježić D., Malčić Ivanišević A., Verzak Ž., Baraba A, & Miletić I. (2018). CYTOTOXICITY AND GENOTOXICITY OF RESIN BASED DENTAL MATERIALS IN HUMAN LYMPHOCYTES IN VITRO. Acta Clinica Croatica, 57(2), 278-285.

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Multimodal Biospecimen Collection Protocol

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Three samples were collected from each patient: tears, saliva and blood (serum). For the tears sample, standard Schirmer test strips (DINA-HITEX, Czech Republic) were used. Schirmer strips were placed in the lower conjunctival sac between the middle and temporal thirds of the lower eyelid of both eyes without topical anesthesia. The strips were transferred to 600 mL of a 0.9% NaCl solution, the solution was centrifuged at 1000 rpm in centrifuge 5430R (Eppendorf AG, Germany) at 4 °C for 5 min and then the supernatant was collected. Saliva samples were collected in a sterile tube (Greiner Bio One, Spain), centrifuged at 300 g at 4 °C in centrifuge 5430R (Eppendorf AG, Germany) for 10 min and diluted in 0.9% NaCl (1:5, saliva:NaCl). Thirdly, serum samples were collected by venopunture employing dry tubes with gelose of 9 mL (Greiner Bio One, Spain) and centrifuged at 3500 rpm at 4 °C in centrifuge Kubota 5910 (Kubota Corporation, Tokyo) for serum collection. All three samples were collected on the same day and were stored at -80 °C until analysis.

Guemes-Villahoz N., Burgos-Blasco B., Echevarria-Torres P.L., Vidal-Villegas B., Rodriguez de la Peña A., Diaz-Valle D., Fernández-Vigo J.I., Sánchez-Ramón S, & Garcia-Feijoo J. (2023). Detection of anti-SARS-CoV-2 antibodies in tears: Ocular surface immunity to COVID-19. Archivos De La Sociedad Espanola De Oftalmologia.

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Collecting Breastmilk Samples for Analysis

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HM samples were collected by mothers at 2 months postpartum. Mothers elected one breast from which to donate the HM sample. Mothers were asked not to breastfeed or express milk from the elected breast for at least two hours prior to sample collection. Mothers washed their hands thoroughly with soap and water and wore disposable nitrile powder-free gloves (Complete Office Supplies, NSW, Australia) during sample collection. The nipple and areola of the expressing breast were cleaned with alcohol and chlorhexidine prep pads (70% isopropyl alcohol and 2% chlorhexidine digluconate, Reynard Health Supplies, NSW, Australia), followed by rinsing with sterile saline solution (Livingstone, NSW, Australia) and drying with sterile gauze swabs (Livingstone, NSW, Australia). Up to 20 mL (otherwise as much as possible) of HM was hand-expressed directly into sterile tubes (Greiner Bio-One, Kremsmünster, Austria). HM samples were stored at 4 °C in the fridge at the home of the participant before being collected within 6–24 h and transported to the laboratory on ice, where they were immediately aliquoted into sterile tubes (Sarstedt, Numbrecht, Germany) and stored at −80 °C until further analysis.
Mothers answered a background questionnaire at the time of recruitment, and an infant and maternal questionnaire on sample collection day.

Cheema A.S., Stinson L.F., Rea A., Lai C.T., Payne M.S., Murray K., Geddes D.T, & Gridneva Z. (2021). Human Milk Lactose, Insulin, and Glucose Relative to Infant Body Composition during Exclusive Breastfeeding. Nutrients, 13(11), 3724.

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Standardized Human Milk Collection for Analysis

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Human milk collection has been described previously [48 (link)]. Briefly, this involved mothers washing their hands thoroughly with soap and water and cleaning the nipple and areola of the expressing breast with alcohol and chlorhexidine prep pads (70% isopropyl alcohol and 2% chlorhexidine digluconate, Reynard Health Supplies, Artarmon, NSW, Australia), followed by rinsing with sterile saline solution (Livingstone, Mascot, NSW, Australia) and drying with sterile gauze swabs (Livingstone, Mascot, NSW, Australia). 10–20 mL of human milk was hand-expressed directly into sterile tubes (Greiner Bio-One, Kremsmünster, Austria). Human milk samples were stored at 4 °C in the fridge at the participant’s home before being collected within 24 h and transported on ice to the laboratory, where they were immediately aliquoted into sterile tubes (Sarstedt, Numbrecht, Germany) and stored at −80 °C until further analysis.

Cheema A.S., Gridneva Z., Furst A.J., Roman A.S., Trevenen M.L., Turlach B.A., Lai C.T., Stinson L.F., Bode L., Payne M.S, & Geddes D.T. (2022). Human Milk Oligosaccharides and Bacterial Profile Modulate Infant Body Composition during Exclusive Breastfeeding. International Journal of Molecular Sciences, 23(5), 2865.

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