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Anti mouse igg subclass specific antibodies

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Anti-mouse IgG subclass-specific antibodies are laboratory reagents used to detect and differentiate between the different subclasses of mouse immunoglobulin G (IgG) in various immunoassays and research applications. These antibodies specifically recognize and bind to the unique epitopes present on the different IgG subclasses, allowing researchers to study the distribution and relative abundance of each subclass in their samples.

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Lab products found in correlation

Pnpp substrate, by Merck Group (3 mentions) Alkaline phosphatase conjugated goat anti mouse igg, by Merck Group (3 mentions) Maxisorp plate, by Thermo Fisher Scientific (3 mentions) Casein, by Thermo Fisher Scientific (3 mentions)

Spelling variants of this product

Specific antibodies (23 citations) Anti-IgG antibodies (6 citations) IgG subclass-specific antibodies (2 citations) Anti-mouse antibodies (2 citations) Anti-IgG mouse (2 citations) Mouse anti (2 citations)

3 protocols using anti mouse igg subclass specific antibodies

1

SARS-CoV-2 Spike Protein IgG Subclass ELISA

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MaxiSorp plates (Nunc) were coated with S1 (monomeric, AA 1–674) or S2 (monomeric, AA 685–1211 The Native Antigen Company; 50 ng/well) or with prefusion stabilized SARS-CoV-2 spike protein (250 ng/well) in PBS for overnight adsorption at 4°C. Plates were washed in PBS/Tween (0.05% v/v) and wells blocked using casein (ThermoFisher Scientific) for 1hr at RT. Serially diluted mouse serum samples were added and incubated overnight at 4°C or 37°C for 2hr for specific IgM detection. Plates were washed and Alkaline Phosphatase-conjugated goat anti-mouse IgG (Sigma) or IgM (Abcam) was added to all wells for 1hr at RT or 2hr at 37°C, respectivelly. After washing pNPP substrate (Sigma) was added. Optical density (OD) values for each well were measured at 405 nm. Endpoint titers were calculated as follows: the log10 OD against log10 sample dilution was plotted and a regression analysis of the linear part of this curve allowed calculation of the endpoint titer with an OD of three times the background. The same calculation was used for diluting the sera to the same amounts of total IgG for further testing on different IgG subclasses with anti-mouse IgG subclass-specific antibodies (Abcam). The results of the IgG subclass ELISA are presented using OD values.
van Doremalen N., Lambe T., Spencer A., Belij-Rammerstorfer S., Purushotham J.N., Port J.R., Avanzato V.A., Bushmaker T., Flaxman A., Ulaszewska M., Feldmann F., Allen E.R., Sharpe H., Schulz J., Holbrook M., Okumura A., Meade-White K., Pérez-Pérez L., Edwards N.J., Wright D., Bissett C., Gilbride C., Williamson B.N., Rosenke R., Long D., Ishwarbhai A., Kailath R., Rose L., Morris S., Powers C., Lovaglio J., Hanley P.W., Scott D., Saturday G., de Wit E., Gilbert S.C, & Munster V.J. (2020). ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques. Nature, 586(7830), 578-582.
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2

SARS-CoV-2 Spike Protein IgG Subclass ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
MaxiSorp plates (Nunc) were coated with S1 (monomeric, AA 1–674) or S2 (monomeric, AA 685–1211 The Native Antigen Company; 50 ng/well) or with prefusion stabilized SARS-CoV-2 spike protein (250 ng/well) in PBS for overnight adsorption at 4°C. Plates were washed in PBS/Tween (0.05% v/v) and wells blocked using casein (ThermoFisher Scientific) for 1hr at RT. Serially diluted mouse serum samples were added and incubated overnight at 4°C or 37°C for 2hr for specific IgM detection. Plates were washed and Alkaline Phosphatase-conjugated goat anti-mouse IgG (Sigma) or IgM (Abcam) was added to all wells for 1hr at RT or 2hr at 37°C, respectivelly. After washing pNPP substrate (Sigma) was added. Optical density (OD) values for each well were measured at 405 nm. Endpoint titers were calculated as follows: the log10 OD against log10 sample dilution was plotted and a regression analysis of the linear part of this curve allowed calculation of the endpoint titer with an OD of three times the background. The same calculation was used for diluting the sera to the same amounts of total IgG for further testing on different IgG subclasses with anti-mouse IgG subclass-specific antibodies (Abcam). The results of the IgG subclass ELISA are presented using OD values.
van Doremalen N., Lambe T., Spencer A., Belij-Rammerstorfer S., Purushotham J.N., Port J.R., Avanzato V.A., Bushmaker T., Flaxman A., Ulaszewska M., Feldmann F., Allen E.R., Sharpe H., Schulz J., Holbrook M., Okumura A., Meade-White K., Pérez-Pérez L., Edwards N.J., Wright D., Bissett C., Gilbride C., Williamson B.N., Rosenke R., Long D., Ishwarbhai A., Kailath R., Rose L., Morris S., Powers C., Lovaglio J., Hanley P.W., Scott D., Saturday G., de Wit E., Gilbert S.C, & Munster V.J. (2020). ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques. Nature, 586(7830), 578-582.
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3

Quantifying Mouse Antibody Response

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MaxiSorp plates (Nunc) were coated with S1 or S2 (The Native Antigen Company; 50 ng/well) in PBS for overnight adsorption at 4°C. Plates were washed in PBS/Tween (0.05% v/v) and wells blocked using casein (ThermoFisher Scientific) for 1hr at RT. Serially diluted mouse serum samples were added and incubated overnight at 4°C. Plates were washed and Alkaline Phosphatase-conjugated goat anti-mouse IgG (Sigma) was added to all wells for 1hr at RT. After washing pNPP substrate (Sigma) was added. Optical density (OD) values for each well were measured at 405 nm. Endpoint titers were calculated as follows: the log10 OD against log10 sample dilution was plotted and a regression analysis of the linear part of this curve allowed calculation of the endpoint titer with an OD of three times the background. The same calculation was used for diluting the sera to the same amounts of total IgG for further testing on different IgG subclasses with anti-mouse IgG subclass-specific antibodies (Abcam). The results of the IgG subclass ELISA are presented using OD values.
van Doremalen N., Lambe T., Spencer A., Belij-Rammerstorfer S., Purushotham J.N., Port J.R., Avanzato V., Bushmaker T., Flaxman A., Ulaszewska M., Feldmann F., Allen E.R., Sharpe H., Schulz J., Holbrook M., Okumura A., Meade-White K., Pérez-Pérez L., Bissett C., Gilbride C., Williamson B.N., Rosenke R., Long D., Ishwarbhai A., Kailath R., Rose L., Morris S., Powers C., Lovaglio J., Hanley P.W., Scott D., Saturday G., de Wit E., Gilbert S.C, & Munster V.J. (2020). ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques. bioRxiv.
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