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Igd buv 737

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The IgD-BUV 737 is a fluorescent label designed for flow cytometry applications. It is used to detect and quantify IgD-positive cells in biological samples. The core function of this product is to provide researchers with a tool to identify and analyze IgD-expressing cells, which can be useful in various immunological studies.

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Lab products found in correlation

Cd14 apc cy7, by BD (3 mentions) Cd38 pe cy7, by BioLegend (3 mentions) Cd19 bv510, by BioLegend (3 mentions) Cd19 apc cy7, by BD (3 mentions) Cd3 apc cy7, by BioLegend (3 mentions) Cd10 pe dazzle594, by BioLegend (3 mentions) Cd21 apc, by BioLegend (3 mentions) Igg percp, by BD (3 mentions) Cxcr5 pe cy7, by BD (3 mentions) Cd3 buv395, by BD (3 mentions) Iga pe, by Miltenyi Biotec (3 mentions) Cd27 bv650, by BioLegend (3 mentions) Pd 1 bv711, by BioLegend (3 mentions) Cd4 buv496, by BD (3 mentions) Cd25 bv421, by BD (3 mentions) Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (3 mentions) Cd8 pe, by BD (3 mentions)

3 protocols using igd buv 737

1

Comprehensive B and T Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Donor PBMCs was stained with live-dead marker (1:1000; Invitrogen, LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit) to gate out the dead cells for 20 min in 4 °C. After washing with FACS buffer, the cells were stained with respective B, T cell marker panel antibodies diluted in FACS buffer (1xPBS, 2% FBS, 0.1% sodium azide) at 4 °C for 30 min. B cell panel: CD19-BV510 (1:40; Biolegend, Cat. No. 363020), CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD3-APC-Cy7 (1:125; Biolegend, Cat. No. 317342), CD10-PE-Dazzle594 (1:50; Biolegend, Cat. No. 312228), CD21-APC (1:50; Biolegend, Cat. No. 354906), CD27-BV650 (1:40; Biolegend, Cat. No. 302828), CD38-PE-Cy7 (1:40; Biolegend, Cat. No. 356608), PD-1-BV711 (1:20; Biolegend, Cat. No. 329928), IgD-BUV 737 (1:40; BD, Cat. No. 612798), IgG-PerCP (1:40; BD), IgA-PE (1:40; Miltenyi, Cat. No. 130-114-002). T cell panel: CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD19-APC-Cy7 (1:50; BD, Cat. No. 557791), CD3-BUV395 (1:40; BD, Cat. No. 563546), CD4-BUV496 (1:40; BD, Cat. No. 612936), CD8-PE (1:40; BD, Cat. No. 555367), CXCR5-PE-Cy7 (1:100; BD, Cat. No. 624052), CD25-BV421 (1:40; BD, Cat. No. 562442), CD127-FITC (1:20; BD, Cat. No. 557938). The samples were then washed, resuspended in FACS buffer and analysed on BD LSRFortessa flow cytometer (Data availability—Figshare 10.6084/m9.figshare.23549964).
Krishnananthasivam S., Li H., Bouzeyen R., Shunmuganathan B., Purushotorman K., Liao X., Du F., Friis C.G., Crawshay-Williams F., Boon L.H., Xinlei Q., Chan C.E., Sobota R., Kozma M., Barcelli V., Wang G., Huang H., Floto A., Bifani P., Javid B, & MacAry P.A. (2023). An anti-LpqH human monoclonal antibody from an asymptomatic individual mediates protection against Mycobacterium tuberculosis. NPJ Vaccines, 8, 127.
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2

Comprehensive B and T Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Donor PBMCs was stained with live-dead marker (1:1000; Invitrogen, LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit) to gate out the dead cells for 20 min in 4 °C. After washing with FACS buffer, the cells were stained with respective B, T cell marker panel antibodies diluted in FACS buffer (1xPBS, 2% FBS, 0.1% sodium azide) at 4 °C for 30 min. B cell panel: CD19-BV510 (1:40; Biolegend, Cat. No. 363020), CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD3-APC-Cy7 (1:125; Biolegend, Cat. No. 317342), CD10-PE-Dazzle594 (1:50; Biolegend, Cat. No. 312228), CD21-APC (1:50; Biolegend, Cat. No. 354906), CD27-BV650 (1:40; Biolegend, Cat. No. 302828), CD38-PE-Cy7 (1:40; Biolegend, Cat. No. 356608), PD-1-BV711 (1:20; Biolegend, Cat. No. 329928), IgD-BUV 737 (1:40; BD, Cat. No. 612798), IgG-PerCP (1:40; BD), IgA-PE (1:40; Miltenyi, Cat. No. 130-114-002). T cell panel: CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD19-APC-Cy7 (1:50; BD, Cat. No. 557791), CD3-BUV395 (1:40; BD, Cat. No. 563546), CD4-BUV496 (1:40; BD, Cat. No. 612936), CD8-PE (1:40; BD, Cat. No. 555367), CXCR5-PE-Cy7 (1:100; BD, Cat. No. 624052), CD25-BV421 (1:40; BD, Cat. No. 562442), CD127-FITC (1:20; BD, Cat. No. 557938). The samples were then washed, resuspended in FACS buffer and analysed on BD LSRFortessa flow cytometer (Data availability—Figshare 10.6084/m9.figshare.23549964).
Krishnananthasivam S., Li H., Bouzeyen R., Shunmuganathan B., Purushotorman K., Liao X., Du F., Friis C.G., Crawshay-Williams F., Boon L.H., Xinlei Q., Chan C.E., Sobota R., Kozma M., Barcelli V., Wang G., Huang H., Floto A., Bifani P., Javid B, & MacAry P.A. (2023). An anti-LpqH human monoclonal antibody from an asymptomatic individual mediates protection against Mycobacterium tuberculosis. NPJ Vaccines, 8, 127.
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3

Comprehensive B and T Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Donor PBMCs was stained with live-dead marker (1:1000; Invitrogen, LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit) to gate out the dead cells for 20 min in 4 °C. After washing with FACS buffer, the cells were stained with respective B, T cell marker panel antibodies diluted in FACS buffer (1xPBS, 2% FBS, 0.1% sodium azide) at 4 °C for 30 min. B cell panel: CD19-BV510 (1:40; Biolegend, Cat. No. 363020), CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD3-APC-Cy7 (1:125; Biolegend, Cat. No. 317342), CD10-PE-Dazzle594 (1:50; Biolegend, Cat. No. 312228), CD21-APC (1:50; Biolegend, Cat. No. 354906), CD27-BV650 (1:40; Biolegend, Cat. No. 302828), CD38-PE-Cy7 (1:40; Biolegend, Cat. No. 356608), PD-1-BV711 (1:20; Biolegend, Cat. No. 329928), IgD-BUV 737 (1:40; BD, Cat. No. 612798), IgG-PerCP (1:40; BD), IgA-PE (1:40; Miltenyi, Cat. No. 130-114-002). T cell panel: CD14-APC-Cy7 (1:50; BD, Cat. No. 557831), CD19-APC-Cy7 (1:50; BD, Cat. No. 557791), CD3-BUV395 (1:40; BD, Cat. No. 563546), CD4-BUV496 (1:40; BD, Cat. No. 612936), CD8-PE (1:40; BD, Cat. No. 555367), CXCR5-PE-Cy7 (1:100; BD, Cat. No. 624052), CD25-BV421 (1:40; BD, Cat. No. 562442), CD127-FITC (1:20; BD, Cat. No. 557938). The samples were then washed, resuspended in FACS buffer and analysed on BD LSRFortessa flow cytometer (Data availability—Figshare 10.6084/m9.figshare.23549964).
Krishnananthasivam S., Li H., Bouzeyen R., Shunmuganathan B., Purushotorman K., Liao X., Du F., Friis C.G., Crawshay-Williams F., Boon L.H., Xinlei Q., Chan C.E., Sobota R., Kozma M., Barcelli V., Wang G., Huang H., Floto A., Bifani P., Javid B, & MacAry P.A. (2023). An anti-LpqH human monoclonal antibody from an asymptomatic individual mediates protection against Mycobacterium tuberculosis. NPJ Vaccines, 8, 127.
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