Macerozyme r 10

Ethanol, mEthanol, chloroform, glacial acetic acid, HNO₃, H₂O₂, Na₂HPO₄, NaH₂PO₄, AgNO₃, Na₂CO₃ and sodium dodecyl sulphate (SDS) were purchased from Merck, Bengaluru, India. Bovine serum albumin and chitosan powder were purchased from Sisco Research Laboratories, Chandigarh, India. Glutaraldehyde was purchased from TCI Chemicals, Chandigarh, India. A Spurr resin kit and OsO₄ were purchased from Electron Microscopy Sciences, Delhi, India. Uranyl acetate was purchased from LobaChemie, India. CuCl, selenourea, oleylamine, citric acid, sodium citrate monobasic, lead citrate, Laemmli buffer, ammonium persulfate, trizma base, tetramethylethylenediamine (TEMED), acrylamide, N,N′- methylenebis(acrylamide), 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), KOH, phenylmethylsulfonyl fluoride (PMSF) cocktail, KH₂PO₄, K₂HPO₄, glycerol, and formaldehyde were purchased from Sigma-Aldrich, Bengaluru, India. Bradford reagent, macerozyme R-10, 1X phosphate buffer saline (PBS), sodium thiosulfate, and ascorbate were purchased from HiMedia, Mumbai, India. Rehydration buffer, IPG strips of pH range 3–10, a ReadyPrep 2D starter kit equilibration buffer I and II, and a ReadyPrep 2D clean-up kit was purchased from Bio-Rad, Gurugram, India. A precision plus protein kaleidoscope prestained protein ladder was purchased from Bio-Rad.

Macerating enzymes, Macerozyme R-10, Nutrient agar, Blood agar, Chocolate agar and MacConkey agar, Mueller–Hinton (MH) agar and potato dextrose agar (PDA) were purchased from Hi-Media Laboratories Pvt. Ltd. Mumbai, India. Silver nitrate (AgNO₃) was acquired from S.D. Fine-Chem. Ltd. Mumbai, India. Throughout the experiment, MiliQ (Millipore^®) water was used for solutions preparation. The antibiotics sensitivity tests were verified using antibiotics disc purchased from Hi-Media Laboratories Pvt. Ltd. Mumbai, India. The identification of clinical cultures was carried out by VITEK^®2 system (Biomerieux Inc. Hazelwood, Mo.) at Anand Laboratory, Surat, India.