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Cy3 conjugated donkey anti rat igg

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Cy3-conjugated donkey anti-rat IgG is a secondary antibody that binds to rat immunoglobulin G (IgG). The antibody is conjugated with the fluorescent dye Cy3, which can be detected using appropriate instrumentation.

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Lab products found in correlation

Cy3 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (3 mentions) Histochoice, by Avantor (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Rat anti mouse mac 2, by Cedarlane (1 mentions) Alexa fluor 488 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) F4 80, by Bio-Rad (1 mentions) Alexa fluor 594 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) A11120, by Thermo Fisher Scientific (1 mentions) Normal goat serum (ngs), by Merck Group (1 mentions) Alexa fluor 488 conjugated anti mouse igg, by Thermo Fisher Scientific (1 mentions) Vectashield, by Vector Laboratories (1 mentions) P80 coilin, by BD (1 mentions) Sc 966, by Santa Cruz Biotechnology (1 mentions) Alexa 488 conjugated donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Sc 7292, by Santa Cruz Biotechnology (1 mentions) Sc 16547, by Santa Cruz Biotechnology (1 mentions) Alexa 488 conjugated donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Sc 6216, by Santa Cruz Biotechnology (1 mentions) Lamin b, by Santa Cruz Biotechnology (1 mentions) Cy3 conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Anti-rat Cy3 (13 citations) Anti-rat IgG Cy3 (6 citations)

6 protocols using cy3 conjugated donkey anti rat igg

1

Conditional Knockout of Mafb in Adipose Tissue

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In this study, we utilized male mice for the hematopoietic system reconstitution and female mice for conditional KO. Epidermal fat pads were collected from wild‐type and Mafb−/− mice, and inguinal and ovary fat pads were collected from Mafbf/f and Mafbf/f::Tie2‐Cre mice. The collected fat pads were either fixed in 4% paraformaldehyde solution and embedded in optimum cutting temperature compound or fixed in neutral‐buffered formalin and embedded in paraffin. Paraffin sections were stained with hematoxylin and eosin (HE), and adipocyte size was measured using imagej analysis software (NIH, Bethesda, MD, USA). The frozen sections were incubated with rabbit anti‐GFP (MBL, Woburn, MA, USA), rabbit anti‐(mouse MafB) (Bethyl, Montgomery, TX, USA) and rat anti‐(mouse Mac‐2) (Cedarlane, Burlington, NC, USA). Alexa Fluor 488‐conjugated goat anti‐(rabbit IgG) (Molecular Probes, Eugene, OR, USA) and Cy3‐conjugated donkey anti‐(rat IgG) (Jackson ImmunoResearch, West Grove, PA, USA) were used as secondary antibodies. Hoechst 33342 (Thermofisher, Rockford, IL, USA) was used to stain nuclei.
Tran M.T., Hamada M., Nakamura M., Jeon H., Kamei R., Tsunakawa Y., Kulathunga K., Lin Y., Fujisawa K., Kudo T, & Takahashi S. (2016). MafB deficiency accelerates the development of obesity in mice. FEBS Open Bio, 6(6), 540-547.
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2

Immunofluorescence analysis of actinic keratosis

Cited 2 times
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Actinic keratosis lesions treated with either metronomic or conventional PDT were harvested at 1-, 3- or 5-days post PDT. Formalin-fixed, paraffin embedded sections were evaluated by Hematoxylin/Eosin (H&E) staining. For immunofluorescent staining of protein markers, skin was embedded in Histochoice (VWR) rather than formalin. For immunofluorescence analyses of Caspase-3 expression and cleavage, the primary antibody specific to cleaved Caspase 3 was purchased from BioVision Inc. and antibodies for macrophages (F4/80) and neutrophils (Ly6G) were from BioRad and Affymetrix, respectively. The secondary antibodies, Cy3-conjugated donkey anti-rabbit IgG and Cy3-conjugated donkey anti-rat IgG were from Jackson ImmunoResearch. Relative expression of marker proteins in sections stained by immunofluorescence was analyzed using fluorescence microscopy at the Lerner Research Institute Image Core [29 (link), 30 (link)].
Anand S., Yasinchak A., Govande M., Shakya S, & Maytin E.V. (2019). Painless versus conventional photodynamic therapy for treatment of actinic keratosis: Comparison of cell death and immune response in a murine model. Proceedings of SPIE--the International Society for Optical Engineering, 10860, 108600K.
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3

Visualizing Germline Granules via CGH-1 Immunostaining

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To visualize germline granules, immunostaining against CGH-1 was performed as previously reported (Navarro et al. 2001 (link)). Briefly the gonads of 1-d-old animals were dissected, freeze-cracked, fixed in cold methanol for 1 min, and then in 3.3% paraformaldehyde for 30 min. For coimmunostaining with anti-CGH-1 and anti-GFP, the samples were fixed for only 18 min. Then, the samples were blocked with 30% normal goat serum (NGS; Sigma-Aldrich, St. Louis, MO) in PBT for 30 min. Primary antibody incubation was performed overnight at 4° with rat anti-CGH-1 (1:25; Navarro et al. 2001 (link)). Coimmunostaining used rabbit anti-CGH-1 (1:1000; Boag et al. 2005 (link)) and mouse anti-GFP (1:5000; A11120 from Molecular Probes, Eugene, OR). Secondary antibody incubations were performed for 1.5 hr at room temperature with Cy3-conjugated donkey anti-rat IgG (1:100; H+L; 112165003, Jackson ImmunoResearch, West Grove, PA), or with Alexa Fluor 594-conjugated anti-rabbit IgG and Alexa Fluor 488-conjugated anti-mouse IgG (1:100; H+L; A11001, Molecular Probes, Eugene, OR). To detect DNA, 1 ng/μl 4′9,6-diamidino-2-phenylindole (DAPI) was used. Vectashield (Vector laboratories, Burlingame, CA) was added to avoid photo bleaching before sealing the sample.
Huelgas-Morales G., Silva-García C.G., Salinas L.S., Greenstein D, & Navarro R.E. (2016). The Stress Granule RNA-Binding Protein TIAR-1 Protects Female Germ Cells from Heat Shock in Caenorhabditis elegans. G3: Genes|Genomes|Genetics, 6(4), 1031-1047.
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4

Immunofluorescence Staining of Nuclear Proteins

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The primary antibodies used were: mouse PML (1:500, SC-966, Santa Cruz), p80 coilin (1:300, #612074, BD Biosciences), hnRNPI (1:300, sc-16547, Santa Cruz), lamin A/C (1:300, sc-7292, Santa Cruz), Sp1 (1:300, sc-16547, Santa Cruz), and lamin B (1:300, sc-6216, Santa Cruz). The following secondary antibodies were used: Alexa 488-conjugated donkey anti-mouse IgG (1:250), Alexa 488-conjugated donkey anti-rabbit IgG (1:250) (Molecular Probes), Cy3-conjugated donkey anti-mouse IgG (1:1000), Cy3-conjugated donkey anti-rat IgG (1:1000), and Cy3-conjugated donkey anti-rabbit IgG (1:1000) (Jackson ImmunoResearch).
Tokunaga K., Saitoh N., Goldberg I.G., Sakamoto C., Yasuda Y., Yoshida Y., Yamanaka S, & Nakao M. (2014). Computational image analysis of colony and nuclear morphology to evaluate human induced pluripotent stem cells. Scientific Reports, 4, 6996.
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5

Immunohistochemical Analysis of Murine AK Lesions

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Murine AK lesions treated with conventional or painless PDT, along with no-PDT controls, were harvested at the times indicated in the figures. Formalin-fixed, paraffin embedded tissue sections were evaluated by Hematoxylin and Eosin (H&E) staining prior to immunohistochemistry (IHC). For immunofluorescent staining of protein markers, tissue samples were fixed in Histochoice (VWR Life Science, Radnor, PA). Antibodies from mouse immune cell phenotyping IHC antibody sampler kit (Cell Signaling Technology; Danvers, MA) were used to analyze macrophages (F4/80), dendritic cells (anti-CD11c) and T cells (CD3, CD8, FoxP3). Other primary antibodies used to analyze neutrophils (Ly6G; Thermo Fisher Scientific; Waltham, MA), calreticulin (CLR), high mobility group protein B1 (HMGB1) and heat shock protein 70 (HSP70) (all three from Cell Signaling), cleaved caspase-3 (BioVision Inc., Milpitas, CA); and secondary antibodies, Cy3-conjugated donkey anti-rabbit IgG and Cy3-conjugated donkey anti-rat IgG (Jackson ImmunoResearch (Westgrove, PA) were from sources indicated. Relative expression levels of marker proteins in tissue sections were analyzed using fluorescence microscopy and quantitated from digital images using IPLab as described above for ROS analysis.
, & Silversides A. (2001). Universities becoming a breeding ground for businesses. CMAJ: Canadian Medical Association Journal, 165(4), 466.
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6

Immunohistochemical Staining of Brain Sections

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Immunohistochemical staining of free floating brain sections was performed on 70 μm coronal brain sections obtained from fixed brains embedded in a 1.5% (w/v) mix of 0.75% Low-Melting Point Agarose (Promega) and 0.75% standard Agarose (Fisher) and cut on a Leica VT 1000P vibratome. Sections were immersed in blocking solution (5% normal donkey serum, 1% BSA, 0.2% glycine, 0.2% lysine with 0.3% TritonX-100 in PBS) and incubated on a rotating shaker for 1 hour at room temperature. Sections were next incubated overnight at 4°C with rat anti-L1-CAM, 1:200 (Millipore, MAB5272MI) diluted in blocking solution. Sections were washed three times with PBS before fluorescent secondary antibody incubation with Cy3-conjugated donkey anti-rat IgG (Jackson). Sections were counterstained with 50μg/ml Hoechst 33342 nuclear stain (Life Tech. #H21492) diluted in 1xPBS for 5 minutes at room temperature and serially mounted onto glass slides with Aquamount.
Robichaux M.A., Chenaux G., Ho H.Y., Soskis M.J., Greenberg M.E., Henkemeyer M, & Cowan C.W. (2015). EphB1 and EphB2 Intracellular Domains Regulate the Formation of the Corpus Callosum and Anterior Commissure. Developmental neurobiology, 76(4), 405-420.
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