Complete easypacks protease inhibitors cocktail

The following reagents were used for biological experiments: RNase (Carl Roth, Karlsruhe, Germany); PhosSTOP™ EASYpachosphatasease inhibitors cocktail and cOmplete™ EASYpacks protease inhibitors cocktail (Roche, Mannheim, Germany); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma, Taufkirchen, Germany); Enzalutamide (Hycultec GmbH, Beutelsbach, Germany); Tariquidar (MedChemExpress, Monmouth Junction, NJ, USA). Primary and secondary antibodies used are listed in Table 2.

The following reagents were used: RNase (Carl Roth, Karlsruhe, Germany); PhosSTOP™ EASYpacks phosphatase inhibitors cocktail and cOmplete™ EASYpacks protease inhibitors cocktail (Roche, Mannheim, Germany); CM-H₂DCFDA (Molecular Probes, Invitrogen, Eugene, OR, USA); N-acetyl cysteine and GSK-690693 (Akt inhibitor) (MedChemExpress, Monmouth Junction, South Brunswick, NJ, USA); Anisomycin (NeoCorp, Weilheim, Germany); carboplatin, cisplatin, and docetaxel (Pharmacy of the University Hospital Hamburg-Eppendorf, Hamburg, Germany); propidium iodide (PI), CCCP (2-[2-(3-chlorophenyl)hydrazinylyidene]propanedinitrile), and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma, Taufkirchen, Germany); enzalutamide (Hycultec GmbH, Beutelsbach, Germany); PD98059 (MEK inhibitor) (Merck Chemicals GmbH, Darmstadt, Germany); SCH772984 (ERK1/2 inhibitors) (Adooq Bioscience, Irvine, CA, USA); olaparib (PARP inhibitor), SP600125 (JNK1/2 inhibitor), and SB203580 (p38 inhibitor) (LC Laboratories, Woburn, MA, USA); annexin-V-FITC (BD Bioscience, San Jose, CA, USA). The primary and secondary antibodies used are listed in Table 3.

Docetaxel was purchased from Pharmacy of the University Hospital Hamburg-Eppendorf (Hamburg, Germany); PhosphoSTOP™ EASYpacks phosphotase inhibitors cocktail and cOmplete™ EASYpacks protease inhibitors cocktail were purchased from Roche (Mannheim, Germany); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma (Taufkirchen, Germany); 3-Methyladenine was purchased from Enzo Life Sciences (Farmingdale, NY, USA); Tariquidar was purchased from MedChemExpress (Monmouth Junction, NJ, USA); Primary and secondary antibodies used for Western blotting are listed in Table 2.

Anisomycin was purchased from NeoCorp (Weilheim, Germany); z-VAD(OMe)-fmk from Enzo Life Sciences (Farmingdale, NY, USA); annexin-V-FITC from BD Bioscience (San Jose, CA, USA); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), doxorubicin, CCCP (2-[2-(3-chlorophenyl)hydrazinylyidene]propanedinitrile), and propidium iodide (PI) from Sigma (Taufkirchen, Germany); cOmplete™ EASYpacks protease inhibitors cocktail and PhosSTOP™ EASYpacks from Roche (Mannheim, Germany); androgen receptor antagonist enzalutamide from Hycultec GmbH (Beutelsbach, Germany). The MEK inhibitor PD98059 was purchased from Merck Chemicals GmbH (Darmstadt, Germany), ERK1/2 inhibitors FR180204 and SCH772984 from Adooq bioscience (Irvine, CA, USA); PARP inhibitor olaparib JNK1/2 inhibitor SP600125 and p38 inhibitor SB203580 from LC Laboratories (Woburn, MA, USA).

Annexin-V-FITC was purchased from BD Bioscience (San Jose, CA, USA). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and propidium iodide (PI) from Sigma (Taufkirchen, Germany). cOmplete™ EASYpacks protease inhibitors cocktail and PhosSTOP™ EASYpacks – from Roche (Mannheim, Germany). Anisomycin was from NeoCorp (Weilheim, Germany). z-VAD(OMe)-fmk was from Enzo Life Sciences (Farmingdale, NY, USA). H₂O₂ was from Carl Roth (Karlsruhe, Germany). CCCP (2-[2-(3-chlorophenyl)hydrazinylyidene]propanedinitrile) was from Sigma (Taufkirchen, Germany). JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide) was from AdiloGen Life Science (Epalinges, Switzerland). N-acetylcystein was from MedChemExpress (Monmouth Junction, NJ, USA). Primary and secondary antibodies used are listed in Table 1.