HaCaT cells were cultured in 24-well plates (1 × 106 cells/well), pretreated with AR extract (10-100 µg/mL) for 24 h, and exposed to UVB. The production of MMP-1 was determined using a commercial ELISA kit (Human total MMP-1 kit; R&D systems, Minneapolis, MN).
Human total mmp 1 kit
Manufactured by R&D Systems
Sourced in United States
The Human total MMP-1 kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human matrix metalloproteinase-1 (MMP-1) in cell culture supernatants, cell lysates, serum, plasma, and other biological fluids.
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Lab products found in correlation
2 protocols using human total mmp 1 kit
1
UVB-Induced MMP-1 Expression in HaCaT Cells
2
Garlic Pretreatment Modulates UVB-Induced MMP-1
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Cells were cultured in 24-well plates (1 × 106 cells/well), pretreated with garlic for 24 h, and exposed to UVB. The production of MMP-1 was determined using a commercial ELISA kit (Human total MMP-1 kit; R&D systems, Minneapolis, MN, USA). MMP-1 level was expressed as a percentage of non-UV irradiated control.
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