Histone h3 xp rabbit mab

CD11b⁺ cells from PBS injected or WGP-injected mice (24 h) were separated using CD11b microbeads (Miltenyi Biotech). Histone was extracted according to the manufacturer’s instruction (Active Motif, Inc.) and protein concentration was determined using Bradford quantification. Histone proteins were separated by SDS-PAGE 15% Tris-HCl gels and transferred onto PVDF membranes (Millipore). The membrane was blocked with 5% BSA at room temperature for 1 h and incubated overnight at 4 °C with primary antibodies including tri-methyl-histone H3 (lys4) rabbit mAb, acetyl-histone H3 (lys27) XP Rabbit mAb, tri-methyl-histone H3 (Lys27) Rabbit mAb, and histone H3 XP rabbit mAb (Cell Signaling Technology), then incubated with HRP-conjugated secondary antibodies (GE Healthcare) at room temperature for 1 h. The membrane was developed with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and detected through Medical Film Processor (Konica Minolta Medical & Graphic). Precision Plus Protein Kaleidoscope Prestained Protein Standards were used as a standard protein marker (Bio-Rad).

Chitosan (deacetylation > 95%) was purchased from Jinhu Crust Product Corp (zi bo, Shandong, China). Chitosanase fermented by Renibacter ium sp.QD1 was obtained from the Ocean University of China. Acrolein was purchased from Xiya Reagent (Chengdu, China). MitoTracker Red CM-H₂Xros and Trizol Reagent were purchased from Invitrogen (Foster City, CA, USA). PrimeScript RT-PCR Kit was purchased from TaKaRa (Dalian, China). The reduced glutathione (GSH) assay kit was purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The MTT cell proliferation and cytotoxicity detection kits, phenyl methane sulfonyl fluoride (PMSF), reactive oxygen species (ROS) detection kit, mitochondrial membrane potential (MMP) detection kit, BCA protein assay kit, CuZn/Mn-SOD assay kit (WST-8), cellular glutathione peroxidase (GPx) assay kit, Nuclear and Cytoplasmic Protein Extraction kit, PVDF membranes, and BCIP/NBT Alkaline Phosphatase Color Development kit were purchased from the Beyotime Institute of Biotechnology (Shanghai, China). Nrf2 XP Rabbit mAb and Histone H3 XP Rabbit mAb were purchased from Cell signaling technology (Boston, MA, USA). All other reagents were obtained from Sigma-Aldrich (Saint Louis, MO, USA), unless otherwise stated.