Tgfbr2fl fl mice

WT C57/BL6 female mice and ICR (CD1) female mice were purchased from the Laboratory Animal Center of the Institute of Genetics and Developmental Biology (Beijing, China). Tgfbr2^fl/fl mice (stock number 012603) were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). Mice with granulosa cell-specific knockout of Tgfbr2 (Tgfbr2^gc−/−) were generated by crossing Tgfbr2^fl/fl mice with previously reported Fshr-Cre mice^{51 (link),52 (link)}. Female mice (21–23 days old) were injected with 5 IU of eCG 48 h before use to stimulate follicle development. In some experiments, the female mice were treated with 5 IU eCG followed by 5 IU hCG to stimulate ovulation. All animal procedures were approved by the Institutional Animal Care and Use Committee of China Agricultural University and maintained according to the Guide for the Care and Use of Laboratory Animals (Institute for Learning and Animal Research at China Agricultural University). The reagents used in this study, unless otherwise stated, were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Dpt^IresCreERT2 mice^{10 (link)} and Lrrc15^DTRGFP mice were designed, generated and bred at Genentech. Tgfbr2^fl/fl mice (012603) were obtained from the Jackson Laboratory. Age- and sex-matched mice (6–12 weeks old) were used for all studies. Mice were maintained under specific pathogen-free conditions using the guidelines of the US National Institutes of Health. The sample sizes for each study are described in the figure legends. All experiments were performed under protocols approved by the Institutional Animal Care and Use Committee at Genentech.