Pe conjugated cd24
PE-conjugated CD24 is a fluorochrome-labeled antibody that binds to the CD24 surface antigen. CD24 is a glycosylphosphatidylinositol (GPI)-anchored sialoglycoprotein that is expressed on a variety of cell types, including hematopoietic cells and some epithelial cells. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CD24-expressing cells using flow cytometry or other fluorescence-based applications.
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6 protocols using pe conjugated cd24
Characterizing Stem Cell Markers
Breg Cell Enumeration by Flow Cytometry
Stemness Marker Analysis of PANC-1 Cells
Cell Surface Marker Profiling by Flow Cytometry
Breast Cancer Cell Immunophenotyping
Stemness and Apoptosis Analysis of Pancreatic Cancer Cells
For analysis of stemness markers, cells (1×106) were labeled with PE‐conjugated CD24 (BD PharMingen™, cat. no. 555428) and APC‐conjugated anti‐CD44 (BD PharMingen™, cat. no. 559942), incubated at 4℃ in the dark for 30 min. Cells were then centrifuged and resuspended in PBS at a concentration of 1×108/ml, and subsequently sorted on a flow cytometer (BD Accuri C6 Plus; BD Biosciences).
For apoptosis and necrosis analysis, cells were cultured for 12 h prior to being treated with gem for a further 24 h. For flow cytometry analysis, cells were detached and labeled using an Annexin V‐PE/7‐AAD Apoptosis Detection kit (Nanjing KeyGen Biotech Co., Ltd.; cat. no. KGA1017) according to the manufacturer's protocol. Apoptotic and necrotic cell subsets were quantified using flow cytometry. A total of 2×104 cells were analyzed per sample. Annexin V‐PE−/7‐AAD− cells were considered as viable, Annexin V‐PE+/7‐AAD− cells were considered as early apoptotic, and Annexin V‐PE+/7‐AAD+ cells were considered as late apoptotic and necrotic cell populations.
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