Hyponatremia was defined as a serum sodium concentration lower than 135 mmol/L and eosinophilia as a count of not less than 500 eosinophils per microliter of blood. TSH was measured with enzyme immunoassay using ST AIA-PACK TSH (TOSOH, Ltd., Tokyo, Japan) on AIA-2000 (TOSOH, Ltd., Tokyo, Japan). FT4 was measured with fluorimetric enzyme-linked immunoassay using ST AIA-PACK FT4 (TOSOH, Ltd., Tokyo, Japan) on AIA-2000 (TOSOH, Ltd., Tokyo, Japan). Low TSH was defined as TSH value below the lower limit of the reference range (TSH reference range 0.38–4.31 μIU/mL) and low fT4 was defined as fT4 value below the lower limit of the reference range (fT4 reference range 0.82–1.63 ng/dL).
Aia 2000
Manufactured by Tosoh
Sourced in Japan, United States
The AIA-2000 is an automated immunoassay analyzer developed by Tosoh. It is designed to perform a variety of immunoassay tests, including those for hormones, tumor markers, and infectious diseases. The AIA-2000 features automated sample handling, incubation, and data processing capabilities to provide accurate and efficient test results.
Automatically generated - may contain errors
10 protocols using aia 2000
1
Hyponatremia and Eosinophilia Evaluation
2
Assessing Menstrual Cycle Phases
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The beginning of the follicular phase was indicated by the onset of menses. To assess the menstrual cycle phase on experimental days ( 31 ), rectal temperature was recorded and venous blood samples were taken for measurement of sex hormones estrogen (17β-estradiol) and progesterone concentration. Rectal temperature was measured in the morning that is about 5-minute after awakening by each subject on the testing day using a thermocouple (Rectal Probe; Ellab, Hvidovre, Denmark; accuracy ±0.01° C) inserted to a depth of 12 cm past the anal sphincter. At the beginning of each experimental trial (Figure 1A), blood samples for estrogen (17βestradiol) and progesterone were collected by venipuncture in vacuum tubes for serum with gel separator (5 ml). Blood samples were allowed to clot and serums were separated by centrifugation (1,200g, 15 minutes) at room temperature. The serum samples were aliquotted and stored at -70° C until analysis. The automated enzyme immunoassay analyzer AIA-2000 (Tosoh Corporation, Tokyo, Japan) was used for blood analysis.
3
Comprehensive Metabolic Profiling of Participants
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The baseline clinical features recorded included age, gender, body height (BH), body weight (BW), systolic blood pressure (SBP), diastolic blood pressure (DBP), waist circumference, and prior history of comorbidities. Body mass index (BMI) was calculated as BW divided by the square of BH (kg/m2). Twenty milliliters of venous blood were collected after 12 h of fasting, and 5 mL of mid-stream urine was collected in the morning. Serum metabolic parameters, including glycated hemoglobin (HbA1c), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), uric acid (UA), and creatinine (Cr) were measured using an automatic analyzer (Toshiba 7600, Japan). Fasting insulin (FINS) and fasting C-peptide (CP) were measured using a chemiluminescence analyzer (Tosoh AIA2000, Japan). HbA1c levels were measured with high-performance liquid chromatography (Tosoh G8, Japan). Urine ACR was measured using an automatic chemiluminescence immunoassay (BioSystems A25, Spain). We calculated homeostasis model assessment—insulin resistance (HOMA-IR) using the following formula [10 (link)]: FINS × FPG/22.5. Ccr was estimated using the Cockcroft–Gault formula in mL/min (for males, [140 − age (years)] × BW (kg)/72 × Cr (mg/dL); for females, [140 − age (year)] × BW (kg)/85 × Cr (mg/dL)).
4
Blood Sample Collection and Analysis
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All subjects had peripheral blood samples taken and drawn into vacutainers, which contained dipotassium ethylenediaminetetraacetic acid (K2EDTA) (BD Vacutainer®; Becton Dickinson UK Ltd., Wokingham, UK). Samples of the routine clinical chemistry assay were directly transported to the hospital laboratory. For the complete blood count test, the XE-5000 (Sysmex, Kobe, Japan) and UniCel® DxH 800 Coulter® Cellular Analysis System automated hematology analyzer (Beckman Coulter, Miami, FL, USA) were utilized. For the total IgE level test, the AIA-2000 automated immunoassay analyzer (Tosoh Bioscience, South San Francisco, CA, USA) was utilized.
5
Routine Clinical Chemistry Tests
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For each routine clinical chemistry test, investigated subjects’ blood samples were transported directly to our hospital laboratory. A complete blood count test was performed with an automated hematology analyzer UniCel® DxH 800 Coulter® Cellular Analysis System (Beckman Coulter, Miami, FL, USA), and immunoglobulin E (IgE) level was measured by automated immunoassay analyzer AIA-2000 (Tosoh Bioscience, South San Francisco, CA, USA).
6
Serum CEA and CA19.9 Biomarker Measurement
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Serum CEA and CA19.9 levels were determined in the Clinical Biochemistry Laboratory at MSKCC using a fluorometric enzyme immunoassay on the Tosoh AIA 2000 (Tosoh USA, Grove City, OH), as part of the patient’s clinical evaluation. Reference ranges for CEA (0–5 ng/ml) and CA19.9 (0–40 ng/ml) were established by the performing laboratory.
7
Serum CEA and CA19.9 Biomarker Measurement
8
Biomarker Assessment in Diabetes
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HbA1c, lipids, and serum creatinine were measured using UniCel DxC 800 Synchron system (Beckman Coulter, Brea, CA, USA). Normal cut-off values for HbA1c were 4–6% (20 mmol/mol–42 mmol/mol). Optimal metabolic control was defined as HbA1c below 7% (53 mmol/mol) without severe hypoglycemias as recommended by International Society for Pediatric and Adolescent Diabetes (ISPAD) [19 (link)]. Normal values for low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), and triglycerides (Tg) were defined as <2.6 mmol/L, >1.1 mmol/L and <1.7 mmol/L, respectively. Normal values for total cholesterol (TCh) were <5.2 mmol/L for patients ≥16 years and <5.5 mmol/L for children under 16 years. At least one abnormal lipid level indicated dyslipidemia. Normal cut-off values for creatinine were 39–91 µmol/L. Cystatin C was measured by an automated enzyme immunoassay system AIA 2000 (TOSOH Corporation, Tokyo, Japan) with a normal range of 0.52–0.97 mg/L.
9
Chemiluminescence Tumor Marker Analysis
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The tumor markers were measured by chemiluminescence using the AIA2000 automatic chemiluminescence analyzer and corresponding reagents (TOSOH Co., Ltd., Tokyo, Japan). Calibrator was the original matched reagent. Internal quality control products were obtained from the Randox (lot 1:1575EC; lot 2:1578EC; Crumline, UK). All EQA samples were provided by the National Center for Clinical Laboratories (lots 201711, 201712, 201713, 201714, 201715).
10
Serum Cortisol and Troponin I Quantification
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Venous blood samples were drawn by the standard venipuncture procedure for each study patient on admission. The samples were allowed to clot at room temperature, and the sera were separated for analyses. The serum concentrations of cortisol and troponin I were quantified by using commercially available kits (ST AIA-PACK CORT and ST AIA-PACK cTnI 3rd Gen, respectively) using the automated enzyme immunoassay analyzer AIA-2000 (Tosoh Corporation, Tokyo, Japan) while following the manufacturer recommendations. The normal value of cortisol was considered as 177–578 nmol/L at 7 a.m., and <434 nmol/L at 4 p.m.
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