The control group culture medium comprised of 75 mIU/mL follicle-stimulating hormone (Livzon Co., Zhuhai, China), 75 mIU/mL luteinizing hormone (Livzon Co., Zhuhai, China), and 10 ng/mL human epidermal growth factor (Sigma, St. Louis, MO, USA). The LPA treatment group culture medium contained of 75 mIU/mL follicle-stimulating hormone, 75 mIU/mL luteinizing hormone, 10 ng/mL human epidermal growth factor, and 10 µM LPA (Sigma, St. Louis, MO, USA).
Human epidermal growth factor (hegf)
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Canada, China, Switzerland
HEGF is a laboratory equipment product manufactured by Merck Group. It functions as a precision instrument for various scientific and research applications. The core purpose of HEGF is to provide accurate and reliable measurements or analysis for users in controlled laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Hydrocortisone, by Merck Group (49 mentions)
Insulin, by Merck Group (39 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (35 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (28 mentions)
Cholera toxin, by Merck Group (22 mentions)
Dmem f12, by Thermo Fisher Scientific (19 mentions)
B27 supplement, by Thermo Fisher Scientific (19 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (13 mentions)
Basic fibroblast growth factor (bfgf), by Merck Group (12 mentions)
Glutamax, by Thermo Fisher Scientific (12 mentions)
Human basic fibroblast growth factor, by Merck Group (10 mentions)
Dexamethasone (dex), by Merck Group (10 mentions)
Penicillin streptomycin, by Merck Group (9 mentions)
L glutamine, by Merck Group (9 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (9 mentions)
L glutamine, by Thermo Fisher Scientific (9 mentions)
Fetal bovine serum (fbs), by Merck Group (9 mentions)
Human insulin, by Merck Group (8 mentions)
Heparin, by Merck Group (8 mentions)
Bovine serum albumin (bsa), by Merck Group (8 mentions)
174 protocols using human epidermal growth factor (hegf)
1
Follicle Stimulation and LPA Treatment
2
Culturing Primary Corticotroph Tumor Cells
3
Culturing Primary Corticotroph Tumor Cells
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Primary human corticotroph tumor and normal pituitary cells were digested and homogenized in 1 mg/mL Clostridium hemolyticum collagenase (Sigma-Aldrich, USA) for 15 min and cultured in RPMI 1640 (Thermo Fischer Scientific, USA) with 10% fetal bovine serum (FBS) (Thermo Fischer Scientific, USA) and 1% penicillin-streptomycin (Gibco, USA) in 5% CO2 at 37°C. Cells incubated with human epidermal growth factor (hEGF) (Sigma-Aldrich, USA) were serum starved using 0% FBS media for two-hours prior to incubating with 100ng of hEGF for 24-h prior to whole-cell lysis.
4
NSCLC Samples and Cell Line Cultivation
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NSCLC samples and paired normal tissues were resected from 33 primary NSCLC patients at Haikou People’s Hospital from 2011 to 2016 and stored at −80 °C. Patients who had received chemotherapy and biological/targeted therapy for NSCLC were excluded from the research. And the patients who were newly diagnosed with histologically confirmed NSCLC were included.
A549 and H1299 cells (two NSCLC cell lines) were procured from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Human bronchial epithelial cells (16HBE) were purchased from Biovector Science Lab, Inc. (Beijing, China). A549 cells were cultivated in a DMEM medium (Thermo Fisher Scientific, Rockford, IL, USA) complemented with 10% FBS (Thermo Fisher Scientific). H1299 cells were cultivated in RPMI-1640 medium (Thermo Fisher Scientific) with 10% FBS (Thermo Fisher Scientific). 16HBE cells were maintained in keratinocyte serum-free medium (Thermo Fisher Scientific) completed with bovine pituitary extract (0.05 mg/mL, Sigma-Aldrich, St. Louis, MO, USA), hydrocortisone (500 ng/mL, Sigma-Aldrich), human epidermal growth factor (5 ng/mL, Sigma-Aldrich) and insulin (0.005 mg/mL, Sigma-Aldrich). All cells were maintained in an incubator containing humidified air and 5% CO2 at 37 °C.
A549 and H1299 cells (two NSCLC cell lines) were procured from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Human bronchial epithelial cells (16HBE) were purchased from Biovector Science Lab, Inc. (Beijing, China). A549 cells were cultivated in a DMEM medium (Thermo Fisher Scientific, Rockford, IL, USA) complemented with 10% FBS (Thermo Fisher Scientific). H1299 cells were cultivated in RPMI-1640 medium (Thermo Fisher Scientific) with 10% FBS (Thermo Fisher Scientific). 16HBE cells were maintained in keratinocyte serum-free medium (Thermo Fisher Scientific) completed with bovine pituitary extract (0.05 mg/mL, Sigma-Aldrich, St. Louis, MO, USA), hydrocortisone (500 ng/mL, Sigma-Aldrich), human epidermal growth factor (5 ng/mL, Sigma-Aldrich) and insulin (0.005 mg/mL, Sigma-Aldrich). All cells were maintained in an incubator containing humidified air and 5% CO2 at 37 °C.
5
Dystrophin Gene Deletion in DMD Cells
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HEK293T cells were obtained from the American Tissue Collection Center (ATCC) through the Duke Cell Culture Facility and were maintained in DMEM supplemented with 10% fetal bovine calf serum and 1% penicillin/streptomycin. Immortalized myoblasts70 (link) from a DMD patient harboring a deletion of exons 48–50 (Δ48–50) in the dystrophin gene were maintained in skeletal muscle media (PromoCell) supplemented with 20% fetal bovine calf serum (Sigma), 50 μg/ml fetuin, 10 ng/ml human epidermal growth factor (Sigma), 1 ng/ml human basic fibroblast growth factor (Sigma), 10 μg/ml human insulin (Sigma), 1% GlutaMAX (Invitrogen), and 1% penicillin/streptomycin (Invitrogen). All cell lines were maintained at 37°C and 5% CO2. HEK293T cells were transfected with Lipofectamine 2000 (Invitrogen) with 400 ng of each expression vector according to the manufacturer’s protocol in 24 well plates. Immortalized myoblasts were transfected with 5 micrograms of each expression vector by electroporation using the Gene Pulser XCell (BioRad) with PBS as an electroporation buffer using optimized conditions 28 (link). Transfection efficiencies were measured by delivering an eGFP expression plasmid (pmaxGFP, Clontech) and using flow cytometry. These efficiencies were routinely ≥ 95% for HEK293T and ≥ 70% for the immortalized myoblasts.
6
Dystrophin Gene Deletion in DMD Cells
7
Culturing TNBC and Normal Mammary Cells
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Triple-negative breast cancer (MDA-MB-231 and CRL-2321) and normal mammary epithelial (MCF10A) cell lines were obtained from the American Type Culture Collection. MCF10A-H cells, derived via H-ras transformation of MCF10A cells, were a kind gift from Dr Barbara Stefanska, Purdue University. MDA-MB-231 cells were cultured in Dublecco's modified essential medium (DMEM; Life Technologies, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Norcross, GA, USA), 100 IU/ml penicillin and 100 µg/ml streptomycin (Life Technologies). MCF10A and MCF10A-H cells were cultured in a 1:1 ratio of DMEM:Ham's F12 supplemented with 5% horse serum (HS; Atlanta Biologicals), 20 ng/ml human epidermal growth factor (Sigma-Aldrich, St. Louis, MO, USA), 0.5 mg/ml hydrocortisone (Sigma-Aldrich), 100 ng/ml cholera toxin (Sigma-Aldrich), 10 µg/ml bovine insulin (Sigma-Aldrich), 100 IU/ml penicillin and 100 µg/ml streptomycin. CRL2321 cells were cultured in Roswell Park Memorial Institute 1640 medium (RPMI-1640, Mediatech Inc., Herndon, VA, USA) supplemented with 10% FBS, 100 IU/ml penicillin and 100 µg/ml streptomycin.
8
Mesenchymal Stem Cell Culture and Lentivirus Transduction
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M-MSCs differentiated from human H9 ESCs [21 (link)] were maintained in EGM2-MV medium (Lonza, San Diego, CA, USA) on rat tail collagen type I (Sigma-Aldrich, St. Louis, MO, USA)-coated plates in a humidified and heated atmosphere of 5% CO2 and 37 °C as previously described [24 (link),26 (link)]. Human UC-MSCs were cultured in low-glucose DMEM containing 10% heat-inactivated FBS, 5 ng/mL human epidermal growth factor (Sigma-Aldrich, St. Louis, MO, USA), 10 ng/mL basic fibroblast growth factor, and 50 ng/mL long-R3 insulin-like growth factor-1 (ProSpec, Rehovot, Israel) as previously described [27 (link)]. Both M-MSCs and UC-MSCs were positive for the expression of CD29, CD73, and CD105 surface molecules but lacked CD14, CD34, and CD45 hematopoietic lineage marker expression (Supplementary Figure S1 ). All M-MSCs were expanded for less than ten passages to ensure multipotency. To induce stable GFP expression, M-MSCs were infected with a GFP-expressing lentivirus, which was generated as described previously [28 (link),29 (link)]. M-MSCs were infected with concentrated lentivirus containing a GFP expression construct using 6 µg/mL polybrene (Invitrogen, Carlsbad, CA, USA), and infected cells were selected using 6 µg/mL blasticidin (Invitrogen).
9
Immortalized Human Neural Progenitor Cells
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Experiments were conducted in the immortalized human neural progenitor ReNcell VM cells derived from the ventral midbrain of a 10-week human fetal neural tissue (Merck Millipore, Billerica, MA, USA). Cells are cultivated in flasks pre-coated with Cultrex Mouse Laminin I (Trevigen, Gaithersburg, Germany), in proliferating medium containing DMEM/F12 medium, B27 neural cell supplement, L-glutamine, gentamycin, human basic fibroblast growth factor (all from Invitrogen, Karlsruhe, Germany), human epidermal growth factor and heparin (all from Sigma-Aldrich, Steinheim, Germany) as described previously [34 (link), 36 (link)]. When 80% cell confluence is reached, differentiation is induced by discarding the proliferating medium followed by washing steps and replacement with differentiating medium i.e. medium without growth factors.
10
Culturing Spherical Cancer Cells
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Cells were seeded as a single cell suspension in 6-well ultra-low attachment plates (Corning) at a density of 5000 cells/well in serum-free DMEM/F12 media (1:1) (Invitrogen), supplemented with basic fibroblast growth factor (10 ng/mL; Sigma-Aldrich), human epidermal growth factor (20 ng/mL; Sigma-Aldrich), and insulin (5 μg/mL; Sigma-Aldrich). Fresh media was added to each well every two days without removing the old media [46 (link)].
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