Blood was collected weekly during treatment by retrobulbar sinus puncture and measured using an ADVIA 2120 system (Siemens Healthcare, Erlangen, Germany).
Advia 2120 system
Manufactured by Siemens
Sourced in Germany
The ADVIA 2120 system is a compact, automated hematology analyzer designed for clinical laboratories. It is capable of performing complete blood count (CBC) tests, including measurements of red blood cells, white blood cells, and platelets. The system provides rapid and accurate results to support clinical decision-making.
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Lab products found in correlation
Advia chemistry system, by Siemens (1 mentions)
Edta tube, by BD (1 mentions)
High sensitivity elisa, by Thermo Fisher Scientific (1 mentions)
Rapidpoint 500 blood gas system, by Siemens (1 mentions)
Elecsys 2010 system, by Roche (1 mentions)
Konelab prime 60i system, by Thermo Fisher Scientific (1 mentions)
6 protocols using advia 2120 system
1
Weekly Blood Monitoring in Treatment
2
Plasma biomarkers and leukocyte profiling
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Plasma ALT/AST levels were determined using the Advia Chemistry System (Siemens), loading 150 μL plasma. Total leukocyte and leukocyte differential cell counts (neutrophils, lymphocytes, monocytes, eosinophils, basophils, and large unstained cells) were assessed using the Advia 2120 system (Siemens), loading 178 μL whole blood. Numbers of animals used are detailed in the table legends. Statistical significance between treatment groups was calculated using ANOVA with Dunnett’s post hoc test.
3
Perioperative Inflammation Biomarkers
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Circulating venous blood was collected in EDTA tubes (BD, US) in the operating room before surgery started. Total white blood cells counts were quantified with an ADVIA-2120 system (Siemens, Germany). The plasma concentration of high sensitivity C Reactive Protein (hsCRP) was determined by ultra-sensitive quantitative turbidimetric test (Bayer Diagnostics, Germany).
4
Venous Blood Sampling and Inflammatory Markers
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Venous blood sampling was performed in supine position, and the prior fasting period was documented. C-reactive protein (CRP) concentration was measured in heparin-plasma by a high-sensitivity latex enhanced immunoturbidimetric assay (Abbott Laboratories, Abbott Park, IL). The limit of detection was 0.2 mg/l. Blood cell counts (including white blood cell count (WBC)) were performed on an ADVIA 2120 system (Siemens Healthcare Diagnostics, Eschborn, Germany). Measures of CRP and WBC were done immediately after sampling.
5
Quantification of Inflammatory Markers
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Total white blood cells counts were quantified with an ADVIA-2120 system (Siemens, Germany). The plasma concentration of C-Reactive Protein (CRP) was determined by ultra-sensitive quantitative turbidimetric test (Bayer Diagnostics, Germany), and those of interleukin (IL)-6 (IL-6) and IL-8 by high sensitivity ELISA (Invitrogen, USA). Blood carboxyhemoglobin levels were determined with a Rapid Point 500 Blood-Gas-System (Siemens, Germany).
6
Assessing Iron Deficiency Biomarkers
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Hemoglobin concentration was measured using the ADVIA 2120 system (Siemens). Anemia was defined according to World Health Organization as hemoglobin concentration < 12 g/dL in women and < 13 g/dL in men. The following blood biomarkers reflecting iron metabolism were measured directly: serum ferritin (μg/L), iron (mg/dL), and total iron binding capacity (TIBC, mg/dL). TSAT was calculated as the ratio of serum iron (mg/dL) and TIBC (mg/dL) multiplied by 100 and expressed as a percentage. Serum ferritin was measured using an immunoassay based on electro-chemiluminescence with the Elecsys 2010 system (Roche). Serum iron and TIBC were assessed using a substrate method with the Konelab Prime 60i system (Thermo Scientific). ID was defined as serum ferritin level < 100 μg/L or serum ferritin 100–299 μg/L in combination with a TSAT < 20% [10 (link), 11 (link)].
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