Anthrone reagent (Thermo Scientific, Waltham, MA, USA), sulfuric acid (HiMedia, Mumbai, India), Congo red (HiMedia), n-butyl alcohol (HiMedia), methanol (HiMedia), hematoxylin (Mayer’s reagent) (HiMedia), acetic anhydride (Sigma-Aldrich, St. Louis, MO, USA), chloroform (HiMedia), ammonium buffer solution (HiMedia), hydrochloric acid (HiMedia), lead(II) acetate trihydrate (HiMedia), ninhydrin (HiMedia), Fehling A and B solutions (HiMedia), potassium ferricyanide (HiMedia), trichloroacetic acid (HiMedia), DPPH (2,2-diphenyl-1-picrylhydrazyl) (Sigma-Aldrich), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) (Invitrogen, Carlsbad, CA, USA), (H2DCFDA) (dichlorofluorescein diacetate) (Invitrogen), LysoTracker Red (Invitrogen), and MitoTracker Red CMX-ROS (Invitrogen).
Sulfuric acid
Manufactured by HiMedia
Sourced in India
Sulfuric acid is a highly corrosive, colorless, and odorless liquid. It is a strong mineral acid with a chemical formula of H2SO4. Sulfuric acid is commonly used in various industrial and laboratory applications, including the production of fertilizers, explosives, and other chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Chloroform, by HiMedia (4 mentions)
Hydrochloric acid (hcl), by HiMedia (4 mentions)
Methanol, by HiMedia (4 mentions)
Sodium hydroxide (naoh), by HiMedia (4 mentions)
Potassium ferricyanide, by HiMedia (3 mentions)
Ferric chloride, by HiMedia (3 mentions)
Glacial acetic acid, by HiMedia (3 mentions)
Sodium carbonate, by HiMedia (3 mentions)
Anthrone reagent, by Thermo Fisher Scientific (2 mentions)
N butyl alcohol, by HiMedia (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (2 mentions)
Ninhydrin, by HiMedia (2 mentions)
Trichloroacetic acid, by HiMedia (2 mentions)
Potassium iodide, by HiMedia (2 mentions)
Ascorbic acid, by HiMedia (2 mentions)
Gallic acid, by HiMedia (2 mentions)
Ethanol, by HiMedia (2 mentions)
Glacial acetic acid, by Merck Group (2 mentions)
Congo red, by HiMedia (1 mentions)
Lysotracker red, by Thermo Fisher Scientific (1 mentions)
13 protocols using sulfuric acid
1
Comprehensive Biochemical Analysis Techniques
2
Bioactive Compound Screening from Microbes
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All reagents used for the experiment were analytical grade purchased from Himedia (HiMedia Laboratories Pvt. Ltd., Mumbai, India) including silver nitrate (Mw: 169.87 g/mol; and purity: ≥99.9%), hydrogen peroxide, iodine, potassium iodide (Mw 166.00 g/mol and purity: 99%), sodium hydroxide (M: 39.997 g/mol and purity: 97%), sulfuric acid, chloroform, benzene, ammonia, naphthol, ethanol, copper acetate, ferric chloride (Mw: 162.2 g/mol and purity: ≥99%), Butylated hydroxytoluene (BHT), hydrochloric acid, potato dextrose agar, nutrient agar, potassium ferricyanide, TCA, α-amylase, etc. Bacterial culture including Bacillus subtilis (MTCC 121T), Shigella flexneri (MTCC 1457), Bacillus megaterium (NCTC 6094), Trichoderma viride, Aspergillus niger (MTCC 12975), and Penicillium crysogenum (NCPF 2802) were collected from Dayanand Science College, Latur, India.
3
Analytical Characterization of Pharmaceutical Compounds
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Glacial acetic acid (Sigma -Aldrich Chemie, Steinheim, Germany), benzene (Nice Laboratory Reagent, Kerala, India), chloroform (Super TeK chemicals, Uttar Pradesh, India), ethanol (Indenta Chemicals, Mumbai, India), ferric chloride (Super Tek Chemicals), lead acetate trihydrate (Guangdong Chemical Reagent Engineering, Guangdong, People’s Republic of China), mercuric chloride (Super Tek Chemicals), methanol (Nice Chemicals, Kochi, India), potassium iodide (Super Tek Chemicals), ranitidine (Cadila Pharmaceuticals, Bengaluru, India), sucralfate (Moraceae Pharmaceuticals Pvt. Ltd, Lucknow, India), sulfuric acid (Hi Media Laboratories Pvt. Ltd, Mumbai, India), hydrochloric acid (Nice Laboratory Reagent), sodium hydroxide (Rankem, Mumbai, India), and phenolphthalein (Fine Chemicals, Mumbai, India) were used.
4
Chitosan-based 5-FU Release Kinetics
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Chitosan, pepsin, potassium monophosphate monobasic, dialysis membrane, CuSO4, sulfuric acid, glacial acetic acid, and ethyl acetate were purchased from HiMedia, India. Sodium tripolyphosphate (STPP), pancreatin, 5-FU, orthophosphoric acid, glacial acetic acid, trichloroacetic acid, and Rd were purchased from Merck (previously Sigma-Aldrich) in the United States. Formic acid, acetonitrile, and p-hydroxydiphenyl were purchased from Loba Chemie. All the reagents used were of analytical grade.
5
Airlift Bioreactor for Lipid Production
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All materials for culture medium (yeast extract, peptone, malt extract, glucose, starch) and other chemicals, such as methanol, sulfuric acid, chloroform, picric acid, sodium carbonate, and sodium sulfate, were bought from HiMedia Laboratories (Mumbai, India). FAME standards were purchased from Sigma-Aldrich (St Louis, MO, USA). The 5-L capacity airlift bioreactor was designed and manufactured at M/S Science World, Coimbatore, India.
6
Phytochemical Screening and Loperamide Evaluation
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Methanol absolute (Loba Chemie), loperamide HCl (Macleods Pharmaceuticals Ltd),
castor oil (Amman Pharmaceutical Industries Co), bisacodyl (Remedica), Tween 80
(Blulux Laboratories), and charcoal (Lab Reagents) were the drugs/chemicals used
in the study. Additionally, the reagents used in phytochemical screening test
included glacial acetic acid (Blulux Laboratories), Mayer’s reagent (Research
Lab Fine Chemical Industries), ammonia (Loba Chemie), ferric chloride (Fisher
Scientific), sulfuric acid (HiMedia Laboratories), and benzene (Blulux
Laboratories).
castor oil (Amman Pharmaceutical Industries Co), bisacodyl (Remedica), Tween 80
(Blulux Laboratories), and charcoal (Lab Reagents) were the drugs/chemicals used
in the study. Additionally, the reagents used in phytochemical screening test
included glacial acetic acid (Blulux Laboratories), Mayer’s reagent (Research
Lab Fine Chemical Industries), ammonia (Loba Chemie), ferric chloride (Fisher
Scientific), sulfuric acid (HiMedia Laboratories), and benzene (Blulux
Laboratories).
7
Antioxidant Activity Evaluation Protocols
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1,1-Diphenyl-2-picrylhydrazyl (DPPH), gallic acid, sodium carbonate, ascorbic acid, potassium dihydrogen phosphate, potassium hydrogen phosphate, potassium ferricyanide, trichloro acetic acid, ferric chloride, 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium persulfate, mercuric chloride, potassium iodide, sulfuric acid, hydrochloric acid, α-naphthol, magnesium ribbons, acetic anhydride, sodium hydroxide, and ninhydrin were purchased from HiMedia Laboratories Pvt. Ltd., India. Folin-Ciocalteu's reagent was obtained from S d fine-Chem. Ltd., India. All the solvents were of analytical grade and purchased from Sisco Research Laboratories Pvt. Ltd., India.
8
Physicochemical Analysis of Castor Oil
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Waste animal bone and teeth were collected from Abattoirs slaughter in Fiche town around Oromia region; Ethiopia. Castor seed oil (Table 1 ), methanol (94%) and ethanol (95%) were purchased from loba Chemie. pvt. Ltd, Mumbai, India. Sulfuric acid (98%), phenolphthalein (99.9%), Iodine monochloride, glacial acetic acid (99.5), Potassium hydroxide, sodium hydroxide, Sodium thiosulfate solution, Chloroform and Hydrochloric (95%) were the product of Himedia Laboratories Pvt Ltd and Sisco Research Laboratories Pvt. Ltd.Table 1
Physico–chemical properties of castor oil.
| Properties | Unit | Value |
|---|---|---|
| Specific gravity at 20 °C | g/cm3 | 0.957–0.961 |
| Acid value | mg of KOH/g | 0.562 |
| Saponification value | mg of KOH/g | 177.43 |
| Iodine value | gI2/100g | 85 |
| pH | – | 6.52 |
| Refractive index | – | 1.456–1.515 |
| Hydroxy value | – | 160–168 |
9
Antioxidant and Antimicrobial Assays
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2,2-Diphenyl-1-picrylhydrazyl, butylated hydroxyl toluene, sodium phosphate, ammonium molybdate, gallic acid, Muller–Hinton agar, Muller–Hinton broth, streptomycin, rifamycin, Sabouraud dextrose agar, Amphotericin B Disc, 7-hydroxy-3H-phenoxazin-3-one-10-oxide, ascorbic acid, ethanol, methanol, and sulfuric acid of analytical grade were purchased from HiMedia.
10
Phytochemical Screening and Toxicity Evaluation
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The study included the following drugs and chemicals: Absolute methanol (Nice Chemicals Pvt Ltd, India), atropine sulfate (Aculife, Health Care Pvt Ltd, India), activated charcoal (SD Fine Chem Ltd, India), loperamide hydrochloride (Remedica, Cyprus), castor oil (Amman Pharmaceutical, Jordan), Wagner's reagent (Research-Lab Fine Chem Industries, India), lead acetate 1% (Guangdong Guanghua chemicals factories, China), sodium hydroxide (BDH, chemical lab, England), ferric chloride (BDH Laboratory Supplies Poole, England), chloroform (Hi-Media Laboratory Reagents, India), and sulfuric acid (HiMedia Laboratories Pvt. Ltd., India). All drugs and chemical reagents used in this study were analytical grade and stemmed from public and private sources.
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