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Montage system

Manufactured by Merck Group
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The Montage system is a laboratory equipment product designed for sample preparation and analysis. It serves as a versatile platform for various laboratory applications. The core function of the Montage system is to facilitate sample handling, processing, and integration with analytical instruments. Further details on the intended use of this product are not available.

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Lab products found in correlation

Multiscreen system, by Merck Group (6 mentions) Big dye chemistry, by Thermo Fisher Scientific (5 mentions) 3730 dna analyzer, by Thermo Fisher Scientific (5 mentions) Sequencher software, by Gene Codes (5 mentions) Seqscape, by Thermo Fisher Scientific (5 mentions)

6 protocols using montage system

1

Sequencing of MAPT Exons 1, 7, 9-13

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Analysis of MAPT exons 1, 7, and 9–13 was performed using primers and conditions that were previously reported (Hutton et al., 1998 (link)). PCR amplicons were purified using the Multiscreen system (Millipore, Billerica, MA, USA) and then sequenced in both directions using Big Dye chemistry following the manufacturer’s protocol (Applied Biosystems, Foster City, CA, USA). Sequence products were purified using the Montage system (Millipore) before being run on an Applied Biosystem 3730 DNA Analyzer. Sequence data were analyzed using either SeqScape (Applied Bio-system) or Sequencher software (Gene Codes, Ann Arbor, MI, USA).
Chen Q., Boeve B.F., Schwarz C.G., Reid R., Tosakulwong N., Lesnick T.G., Bove J., Brannelly P., Brushaber D., Coppola G., Dheel C., Dickerson B.C., Dickinson S., Faber K., Fields J., Fong J., Foroud T., Forsberg L., Gavrilova R.H., Gearhart D., Ghoshal N., Goldman J., Graff-Radford J., Graff-Radford N.R., Grossman M., Haley D., Heuer H.W., Hsiung G.Y., Huey E., Irwin D.J., Jack C.R., Jones D.T., Jones L., Karydas A.M., Knopman D.S., Kornak J., Kramer J., Kremers W., Kukull W.A., Lapid M., Lucente D., Lungu C., Mackenzie I.R., Manoochehri M., McGinnis S., Miller B.L., Pearlman R., Petrucelli L., Potter M., Rademakers R., Ramos E.M., Rankin K.P., Rascovsky K., Sengdy P., Shaw L., Syrjanen J., Tatton N., Taylor J., Toga A.W., Trojanowski J., Weintraub S., Wong B., Boxer A.L., Rosen H., Wszolek Z, & Kantarci K. (2019). Tracking white matter degeneration in asymptomatic and symptomatic MAPT mutation carriers. Neurobiology of aging, 83, 54-62.
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2

Genetic Screening of GRN Mutation Families

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All participants were part of known GRN families. We specifically sequenced the exon harboring the known GRN gene mutation observed in their families using the previously published protocol (Baker et al., 2006 (link); Gass et al., 2006 (link)). Exons 0–12 and the 3′ untranslated region of the GRN gene were amplified by polymerase chain reaction (PCR) assay. The PCR amplicons were purified using the Multiscreen system (Millipore, Billerica, MA) and then sequenced in both directions using Big Dye chemistry following the manufacturer’s protocol (Applied Biosystems, Foster City, CA). Sequence products were purified using the Montage system (Millipore) before being run on an Applied Biosystem 3730 DNA Analyzer. Sequence data were analyzed using either SeqScape (Applied Biosystems) or Sequencher software (Gene Codes, Ann Arbor, MI). Furthermore, sequencing is also performed to detect variants in the following genes MAPT, C9orf72, TARDBP, PSEN1, PSEN2 and APP according to the LEFFTDS protocol.
Chen Q., Boeve B.F., Senjem M., Tosakulwong N., Lesnick T., Brushaber D., Dheel C., Fields J., Forsberg L., Gavrilova R., Gearhart D., Graff-Radford J., Graff-Radford N., Jack CR J.r., Jones D., Knopman D., Kremers W.K., Lapid M., Rademakers R., Ramos E.M., Syrjanen J., Boxer A.L., Rosen H., Wszolek Z.K, & Kantarci K. (2019). Trajectory of Lobar Atrophy in Asymptomatic and Symptomatic GRN Mutation Carriers: a Longitudinal MRI Study. Neurobiology of aging, 88, 42-50.
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3

Genetic Testing for lvPPA Patients

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All six lvPPA patients without β-amyloid deposition underwent apolipoprotein E (APOE) genotype testing, as previously described [30 (link)], and were tested for the presence of GRN gene mutations. Exons 0–12 and the 3′ untranslated region of the GRN gene were amplified by polymerase chain reaction (PCR) assay using our previously published primers and protocol [31 (link), 32 (link)]. The PCR amplicons were purified using the Multiscreen system (Millipore, Billerica, MA) and then sequenced in both directions using Big Dye chemistry following the manufacturer’s protocol (Applied Biosystems, Foster City, CA). Sequence products were purified using the Montage system (Millipore) prior to being run on an ABI 3730 DNA Analyzer. Sequence data was analyzed using either SeqScape or Sequencer software.
Josephs K.A., Duffy J.R., Strand E.A., Machulda M.M., Vemuri P., Senjem M.L., Perkerson R.B., Baker M.C., Lowe V., Jack CR J.r., Rademakers R, & Whitwell J.L. (2014). Progranulin-associated PiB-negative logopenic primary progressive aphasia. Journal of neurology, 261(3), 604-614.
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4

Genetic Sequencing of FTLD Families

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Participants recruited into this study are part of known MAPT families. For each of the participants, we specifically sequence the exon harboring the known MAPT mutation observed in that family using previously published protocol [29] (link). Sequencing is also performed to detect variants in the following genes GRN, C9ORF72, TARDBP, PSEN1, PSEN2, and APP according to the LEFFTDS protocol. PCR amplicons were purified using the Multiscreen system (Millipore, Billerica, MA) and then sequenced in both directions using Big Dye chemistry following the manufacturer's protocol (Applied Biosystems, Foster City, CA). Sequence products were purified using the Montage system (Millipore) before being run on an Applied Biosystem 3730 DNA Analyzer. Sequence data were analyzed using either SeqScape (Applied Biosystem) or Sequencher software (Gene Codes, Ann Arbor, MI).
Chen Q., Boeve B.F., Senjem M., Tosakulwong N., Lesnick T.G., Brushaber D., Dheel C., Fields J., Forsberg L., Gavrilova R., Gearhart D., Graff-Radford J., Graff-Radford N.R., Jack CR J.r., Jones D.T., Knopman D.S., Kremers W.K., Lapid M., Rademakers R., Syrjanen J., Boxer A.L., Rosen H., Wszolek Z.K, & Kantarci K. (2019). Rates of lobar atrophy in asymptomatic MAPT mutation carriers. Alzheimer's & Dementia : Translational Research & Clinical Interventions, 5, 338-346.
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5

MAPT Exons Sequence Analysis

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Analysis of MAPT exons 1, 7, and 9‐13 was performed using primers and conditions that were previously published.10 PCR amplicons were purified using the Multiscreen system (Millipore, Billerica, MA) and then sequenced in both directions using Big Dye chemistry following the manufacturer's protocol (Applied Biosystems, Froster City, CA). Sequence products were purified using the Montage system (Millipore) before being run on an Applied Biosystem 3730 DNA Analyzer. Sequence data were analyzed using either SeqScape (Applied Biosystem) or Sequencher software (Gene Codes, Ann Arbor, MI).
Chen Q., Boeve B.F., Tosakulwong N., Lesnick T., Brushaber D., Dheel C., Fields J., Forsberg L., Gavrilova R., Gearhart D., Haley D., Gunter J.L., Graff‐Radford J., Jones D., Knopman D., Graff‐Radford N., Kraft R., Lapid M., Rademakers R., Wszolek Z.K., Rosen H., Boxer A.L, & Kantarci K. (2019). Brain MR Spectroscopy Changes Precede Frontotemporal Lobar Degeneration Phenoconversion in Mapt Mutation Carriers. Journal of Neuroimaging, 29(5), 624-629.
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6

Sequencing Analysis of MAPT Exons

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Analysis of MAPT exons 1, 7 and 9–13 was performed using primers and conditions that were previously published.10 (link) PCR amplicons were purified using the Multiscreen system (Millipore, Billerica, MA) and then sequenced in both directions using Big Dye chemistry following the manufacturer’s protocol (Applied Biosystems, Froster City, CA). Sequence products were purified using the Montage system (Millipore) before being run on an Applied Biosystem 3730 DNA Analyzer. Sequence data were analyzed using either SeqScape (Applied Biosystem) or Sequencher software (Gene Codes, Ann Arbor, MI).
Chen Q., Boeve B.F., Tosakulwong N., Lesnick T., Brushaber D., Dheel C., Fields J., Forsberg L., Gavrilova R., Gearhart D., Haley D., Gunter J.L., Graff‐Radford J., Jones D., Knopman D., Graff‐Radford N., Kraft R., Lapid M., Rademakers R., Wszolek Z.K., Rosen H., Boxer A.L, & Kantarci K. (2019). Brain MR Spectroscopy Changes Precede Frontotemporal Lobar Degeneration Phenoconversion in Mapt Mutation Carriers. Journal of Neuroimaging, 29(5), 624-629.
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