Acuri c6 flow cytometer

Manufactured by BD

Sourced in United States

The Acuri C6 is a flow cytometer manufactured by BD. It is a compact and versatile instrument designed for various applications, including cell analysis, sorting, and phenotyping. The Acuri C6 utilizes laser excitation and detectors to measure and analyze the physical and fluorescent characteristics of cells or particles passing through a fluid stream.

Automatically generated - may contain errors

Lab products found in correlation

Cellometer auto t4 cell counter, by Revvity (2 mentions) Evos fl cell imaging system, by Thermo Fisher Scientific (2 mentions) Percp cy5, by BD (1 mentions) Vacutainer, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) Fcap array software, by BD (1 mentions) Celltracker green cmfda, by Thermo Fisher Scientific (1 mentions) Cyan adp cell analyzer, by Beckman Coulter (1 mentions)

11 protocols using acuri c6 flow cytometer

Phenotypic analysis of human dendritic cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

DCs were stained by antibodies against human CD14 labeled by FITC, CD1a labeled by phycoerythrin (PE), CD83 labeled allophycocyanin (APC) and CD86 labeled PerCP-Cy™5.5 (BD Biosciences, San Jose, CA). The expression of each molecule was analyzed using an Acuri C6 flow cytometer (BD Biosciences).

Chang W.A., Hung J.Y., Jian S.F., Lin Y.S., Wu C.Y., Hsu Y.L, & Kuo P.L. (2016). Laricitrin ameliorates lung cancer-mediated dendritic cell suppression by inhibiting signal transducer and activator of transcription 3. Oncotarget, 7(51), 85220-85234.

+ Open protocol

+ Expand

Camel Leukocyte Apoptosis and Necrosis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell necrosis was measured by the dye exclusion assay [26 (link)]. Briefly, camel leukocytes were labeled with propidium iodide (PI) at a final concentration of 2 µg/mL (Calbiochem, Germany) and PI-fluorescence was measured by flow cytometry (detected in FL-3). PI-positive dead cells with permeable cell membranes were distinguished from PI-negative live cells. For the measurement of cell apoptosis, separated leukocytes (100µL in RPMI-1640 cell culture medium) were incubated with JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide) in a 96-well microtiter plate [27 (link),28 (link),29 (link)]. JC-1 solution (100µL of 2μmol/L final concentration) was added to the cells in each well for 15 min (5% CO2) at 37 °C. Finally, labeled cells were washed twice with PBS, suspended in 200μL PBS, and analyzed on the Acuri C6 flow cytometer (BD Biosciences). Apoptotic cells with JC-1 monomers (increased green fluorescence) were distinguished from normal non-apoptotic cells with orange JC-1 aggregations (increased orange fluorescence in FL-2). Hydrogen peroxide (H2O2; 180 uM)) has been used to establish a positive control of apoptosis.

Hussen J., Kandeel M., Shawaf T., Al-Mubarak A.I., Al-Humam N.A, & Almathen F. (2021). Immunomodulatory Effects of the Cyclooxygenase Inhibitor Lornoxicam on Phenotype and Function of Camel Blood Leukocytes. Animals : an Open Access Journal from MDPI, 11(7), 2023.

+ Open protocol

+ Expand

Antibody Binding Assay on TF-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

TF-1 cells (1.5^*10⁵) in a volume of 150 μl were incubated with the indicated concentrations of A2, H3 or pTACE in RPMI 1% FBS for 1 h at 37°C. Next, the cell were centrifuged at 200 rcf for 5 min at 4°C and washed three times with 200 μl PBS, 1% BSA. The washed cells were re-suspended in 50 μl PBS containing 1% BSA and goat anti-human IgG Allophycocyanin (APC) conjugate (Jackson immuneresearch) was added to a final concentration of 10 μg/ml. The cells were incubated for 30 min at RT and then washed three times with PBS containing 1% BSA. The cells where resuspended in 500 μl of PBS containing 1% BSA and analyzed on Acuri C6 flow cytometer (BD Biosciences).

Weizman T., Levin I., Zaretsky M., Sagi I, & Aharoni A. (2017). Increased Potency of a Bi-specific TL1A-ADAM17 (TACE) Inhibitor by Cell Surface Targeting. Frontiers in Molecular Biosciences, 4, 61.

+ Open protocol

+ Expand

Flow Cytometric Analysis of Eosinophil Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For flow cytometric analysis, blood was collected in BD Vacutainer containing ethylenediaminetetracetic acid (EDTA) to prevent coagulation. The samples were processed within 6 hours of collection to reduce activation of eosinophils after collection. The samples were incubated for 30 minutes at 4°C with Anti-CD69 mAb bound to fluorescein isothiocyanate (FN50, mIgG1, BD Pharmigen), Anti- FcR1 mAb bound to allophycocyanin (AER-37, mIgG2, eBioscience), Anti-CD9 mAb bound to R-phycoerythrin (eBioSN4, mIgG1, eBiosciences), Anti-CD16 mAb bound to peridinin chlorophyll protein (3G8, mIgG1, Invitrogen). Samples were lysed using FACS Lysing Solution (BD Biosciences) following manufacturer’s instructions. Cells were washed using PBS solution and suspended in 1% formaldehyde solution and analyzed on an AcuriC6 Flow Cytometer (BD Biosciences).

Loizou D., Enav B., Komlodi-Pasztor E., Hider P., Kim-Chang J., Noonan L., Taber T., Kaushal S., Limgala R., Brown M., Gupta R., Balba N., Goker-Alpan O., Khojah A, & Alpan O. (2015). A Pilot Study of Omalizumab in Eosinophilic Esophagitis. PLoS ONE, 10(3), e0113483.

+ Open protocol

+ Expand

Exosome-mediated Rhodamine 123 Delivery in Brain Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorescent rhodamine 123 was used as a marker of exosome delivery and the transport mechanism for delivery from brain endothelial bEND.3 cell released exosomes was evaluated (22 ). The brain endothelial bEND.3 cells were seeded on a 100 mm petri dish with 10 ml of cell media at a density of 2×10⁶ cells/ml counted by a Cellometer® Auto T4 Cell Counter (Nexcelom Bioscience LLC, Lawrence, MA, USA). After 2 h of incubation at 37 or 4°C, the buffer, 0.2 mg/ml rhodamine 123 alone or 0.2 mg/ml rhodamine 123 formulated with bEND.3 released exosomes (200 μg/ml total proteins) was added into the media. After incubation, the media with treatment solutions were removed and the cells were washed with 1× PBS three times. Cells were scraped, suspended, and diluted to 1×10⁶ cells/ml and then analyzed with an Acuri C6 flow cytometer (BD Biosciences, San Jose, CA, USA) using the FL3 detector position.

Yang T., Martin P., Fogarty B., Brown A., Schurman K., Phipps R., Yin V.P., Lockman P, & Bai S. (2015). Exosome Delivered Anticancer Drugs Across the Blood-Brain Barrier for Brain Cancer Therapy in Danio Rerio. Pharmaceutical research, 32(6), 2003-2014.

+ Open protocol

+ Expand

Cytokine Profiling of Conditioned Media

Check if the same lab product or an alternative is used in the 5 most similar protocols

Conditioned media samples from pericytes and microglia treated as above were spun at 200×g for 5 min and the supernatant collected and stored at − 20 °C. The concentration of cytokines was measured using a cytometric bead array (CBA) (BD Biosciences, CA, USA) as per manufacturer’s instructions. CBA samples were run on an Acuri C6 flow cytometer (BD Biosciences, CA, USA). Data were analysed using FCAP-array software (version 3.1; BD Biosciences, CA, USA) to convert fluorescent intensity values to concentrations using an 11-point standard curve (0–10,000 pg/mL) (Supplementary Table 4).

Stevenson T.J., Johnson R.H., Savistchenko J., Rustenhoven J., Woolf Z., Smyth L.C., Murray H.C., Faull R.L., Correia J., Schweder P., Heppner P., Turner C., Melki R., Dieriks B.V., Curtis M.A, & Dragunow M. (2022). Pericytes take up and degrade α-synuclein but succumb to apoptosis under cellular stress. Scientific Reports, 12, 17314.

+ Open protocol

+ Expand

Quantifying P-Glycoprotein Substrate Uptake

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The internalization of fluorescent rhodamine 123, a P-gp substrate, was evaluated in wild-type and CRISPR/Cas9 transfected brain endothelial bEND.3 cells as described previously (Yang et al., 2014b ). Brain endothelial bEND.3 cells were seeded on a 6-well plate with 2 mL of cell media. After 24 hours of incubation at 37°C and 5% CO₂, formulations including 0.2 mg/mL rhodamine 123 were added to the media. After 18 hours of incubation at 37°C, the media with treatment solutions were removed and the cells were washed with PBS three times. Cells were fixed in paraformaldehyde solution and permeabilized in Triton X-100. The final cell samples were imaged using an EVOS^® FL cell imaging system (Life Technologies, Waltham, MA) (Yang et al., 2015 (link), Yang et al., 2017 (link)). After imaging, cells were scraped, suspended, and diluted to 1x10⁶ cells/mL and then analyzed with an Acuri C6 flow cytometer (BD Biosciences, San Jose, CA, USA).

Yang T., Curtis S., Bai A., Young A., Derosier D., Ripley S, & Bai S. (2022). CRISPR/Cas9 Targeting Liposomes Knocked down Multidrug Resistance Proteins in Brain Endothelial Cells as a Model to Predict Potential Pharmacoresistance. Colloids and surfaces. B, Biointerfaces, 222, 113103.

+ Open protocol

+ Expand

Fluorescent Liposome Uptake in Mouse Brain Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse brain endothelial bEND.3 cells were grown in recommended Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 100 μg/mL penicillin plus 100 μg/mL streptomycin, in a humidified 37°C incubator with 5% CO₂. The bEND.3 cells were seeded on a 100 mm petri dish at a density of 2×10⁶ cells/mL counted by a Cellometer^® Auto T4 Cell Counter (Nexcelom Bioscience LLC, Lawrence, MA, USA). After 24 hours of incubation at 37°C and 5% CO₂, cells were treated with targeted or non-targeted fluorescent liposomes for 48 hours. The cells were washed three times with 1x PBS and then imaged using an EVOS^® FL cell imaging system (Life Technologies, Waltham, MA). After imaging, cells were scraped, suspended, and diluted to 1x10⁶ cells/mL and then analyzed with an Acuri^™ C6 flow cytometer (BD Biosciences, San Jose, CA, USA) and WinListTM 3D Flow Cytometry Analysis Software (Verity, Topsham, ME, USA) (Yang et al., 2017 (link)).

+ Open protocol

+ Expand

Quantifying Epibiotic Cells on Algae

Check if the same lab product or an alternative is used in the 5 most similar protocols

Epibiotic cells were collected at the surface of algal samples by gently scraping the thalli with a sterile scalpel. Heteroprokaryotic cell densities and cell abundance in seawater samples were determined after fixation, extraction, and staining (details in SI). Analyses were performed with a BD Acuri C6 flow cytometer (BD Biosciences, San Jose, CA, USA). Densities at the algal surface were expressed using the measured surface of each frond (details in SI and Fig. S1).

Paix B., Layglon N., Le Poupon C., D’Onofrio S., Misson B., Garnier C., Culioli G, & Briand J.F. (2021). Integration of spatio-temporal variations of surface metabolomes and epibacterial communities highlights the importance of copper stress as a major factor shaping host-microbiota interactions within a Mediterranean seaweed holobiont. Microbiome, 9, 201.

+ Open protocol

+ Expand

Cytotoxic NK Cell Conjugate Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five million expanded NK cells were labeled with eFluor 670 (APC) (eBioscience), and 5×10⁶ ALL cells were labeled with CellTracker Green CMFDA (FITC) (Invitrogen), according to the manufacturer’s protocol. Cells were co-incubated at 37°C for 30 minutes at a ratio of 2:1 (NK:tumor). The proportion of double-positive cells (conjugate formation) was analyzed using BD Acuri C6 flow cytometer.

Vicioso Y., Gram H., Beck R., Asthana A., Zhang K., Wong D.P., Letterio J, & Parameswaran R. (2019). Combination therapy for treating advanced drug-resistant acute lymphoblastic leukemia. Cancer immunology research, 7(7), 1106-1119.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!