All crosses and staging were performed at 25 °C. Pupal development was expressed as hours after puparium formation (APF) where white pre-pupae were defined as 0 h APF. Stocks used were: GMR.wg ((Wehrli and Tomlinson, 1998 (link))), Tub-α1 > w + > wg (Wehrli and Tomlinson, 1998 (link)) UAS-GFP (Johnston and Sanders, 2003 (link)), UAS-arm* (Zecca et al., 1996 (link)) esg-LacZ, wg-lacZ, UAS-Esg-RNAi (TRiP stock BL# 28514), pros-Gal4 (gift of Tiffany Cook) LongGMR-Gal4 (Wernet et al., 2003 (link)) (All from Bloomington Stock Center). GMR.flip (generated by Y. Mavromatakis), UAS-wg-RNAi (Gift from G. Struhl), d -APCQ8, elav(C155)-Gal4 (Ahmed et al., 1998 (link)).
Pros gal4
Manufactured by Bloomington Drosophila Stock Center
Pros-Gal4 is a genetic tool used in Drosophila research. It is a promoter-driven Gal4 construct that drives expression of target genes in the Drosophila nervous system.
Automatically generated - may contain errors
2 protocols using pros gal4
1
Genetic Manipulation of Wingless Signaling
2
Drosophila Genetic Tools for Live Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NRE-GFP (BL30727), 20×QUAS-6×mcherry (BL52268), pros-Gal4 (BL84276), UAS-his-RFP (BL56555), 20×UAS-IVS-GCaMP6s (BL42749), and Rab3-YFP (BL62541), vn-lacZ (BL11749) were obtained from the Bloomington Stock Center. pros-QF2 was generated from pros-Gal4 by the homology assisted CRISPR knock-in (HACK) method35 (link). esg-Gal4, UAS-mCD8:GFP, Rab3-Gal4, esg-GFP, and ubi-H2B:RFP were kindly donated from the laboratory of Benjamin Ohlstein, while 10×UAS-mry:tdTomato was kindly donated from the laboratory of Ken Irvine. Upd3-LacZ was kindly donated from the laboratory of Bruce A. Edgar.
We performed all Drosophila crosses at 25 °C. The number of females was no more than 30 for each cross to maintain moderate body size, and they were transferred to new bottles containing fresh food every 2 days. Drosophila food was composed of a mix of 12 L water, 0.9 kg cornmeal, 0.12 kg soybean meal, 0.1 kg agar, 0.21 kg yeast, 0.8 l barley malt syrup, and 150 ml 10 % nipagin in ethanol. We collected the females after eclosion and placed them with males in a new vial at 25 °C. We supplied fresh food vials every 2–3 days and we used the 7-day old or 14 days old mated females in the experiments.
We performed all Drosophila crosses at 25 °C. The number of females was no more than 30 for each cross to maintain moderate body size, and they were transferred to new bottles containing fresh food every 2 days. Drosophila food was composed of a mix of 12 L water, 0.9 kg cornmeal, 0.12 kg soybean meal, 0.1 kg agar, 0.21 kg yeast, 0.8 l barley malt syrup, and 150 ml 10 % nipagin in ethanol. We collected the females after eclosion and placed them with males in a new vial at 25 °C. We supplied fresh food vials every 2–3 days and we used the 7-day old or 14 days old mated females in the experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!