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Round bottom facs

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The Round-Bottom FACS is a type of flow cytometry instrument used for the analysis and sorting of cells or other particles. It is designed with a round-shaped flow cell that allows for efficient hydrodynamic focusing and accurate detection of target populations.

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Lab products found in correlation

Facsdiva software, by BD (2 mentions) Sodium azide, by Merck Group (2 mentions) Human serum, by Lonza (2 mentions) Facscanto flow cytometer, by BD (2 mentions)

Spelling variants of this product

BD FACSTM (2 citations) 12 × 75 mm round bottom FACS tube (2 citations)

2 protocols using round bottom facs

1

Endocytosis and Allergen Internalization in MoDCs

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MoDCs were harvested by scraping, washed, counted and dispensed at 2.5 × 105 cells per tube into 5 ml polystyrene Round-Bottom FACS (Fluorescence Activated Cell Sorting) tubes (BD Biosciences). Then, cells were treated with 10 μg/ml Cy3-Phl p 5 or AlexaFluor488-SF-nsLTP or both labeled allergens. Alternatively, cells were incubated with 10 μg/ml FITC-transferrin or 500 μg/ml FITC-dextran, to investigate receptor-mediated and fluid-phase endocytosis or were used without any treatment. The cells were incubated at 37 °C for 5, 10, 30, 60 and 120 min. In another set of experiments, MoDCs were treated with 0.5, 1 and 2 μg/ml AlexaFluor488-labeled wild type or highly mannosidic HA antigens for 1, 5, and 15 min at 37 °C. The cells were then washed with serum free IMDM and the cell pellet was dissolved in FACS buffer consisting of PBS, 1% BSA, 0.1% sodium azide (Merck, www.merck.de), 2% FCS and 1% human serum (Lonza). The samples were analyzed with a minimum of 10,000 events on a FACS Canto flow cytometer using the BD FACS Diva software (BD Biosciences).
Ashjaei K., Palmberger D., Bublin M., Bajna E., Breiteneder H., Grabherr R., Ellinger I, & Hoffmann-Sommergruber K. (2015). Atopic donor status does not influence the uptake of the major grass pollen allergen, Phl p 5, by dendritic cells. Journal of immunological methods, 424, 120-130.
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2

Endocytosis and Allergen Internalization in MoDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
MoDCs were harvested by scraping, washed, counted and dispensed at 2.5 × 105 cells per tube into 5 ml polystyrene Round-Bottom FACS (Fluorescence Activated Cell Sorting) tubes (BD Biosciences). Then, cells were treated with 10 μg/ml Cy3-Phl p 5 or AlexaFluor488-SF-nsLTP or both labeled allergens. Alternatively, cells were incubated with 10 μg/ml FITC-transferrin or 500 μg/ml FITC-dextran, to investigate receptor-mediated and fluid-phase endocytosis or were used without any treatment. The cells were incubated at 37 °C for 5, 10, 30, 60 and 120 min. In another set of experiments, MoDCs were treated with 0.5, 1 and 2 μg/ml AlexaFluor488-labeled wild type or highly mannosidic HA antigens for 1, 5, and 15 min at 37 °C. The cells were then washed with serum free IMDM and the cell pellet was dissolved in FACS buffer consisting of PBS, 1% BSA, 0.1% sodium azide (Merck, www.merck.de), 2% FCS and 1% human serum (Lonza). The samples were analyzed with a minimum of 10,000 events on a FACS Canto flow cytometer using the BD FACS Diva software (BD Biosciences).
Ashjaei K., Palmberger D., Bublin M., Bajna E., Breiteneder H., Grabherr R., Ellinger I, & Hoffmann-Sommergruber K. (2015). Atopic donor status does not influence the uptake of the major grass pollen allergen, Phl p 5, by dendritic cells. Journal of immunological methods, 424, 120-130.
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