XAV939, an inhibitor of TNKS activity, Salinomycin, an inhibitor of the Wnt pathway that decreases the phosphorylation of low-density lipoprotein receptor-related protein 6, and ABT-888, PARP1 and PARP2 inhibitor, were obtained from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in water or DMSO. A stock of 10 mM was stored at −80 °C. The 50% inhibitory concentration (IC50) of XAV939 for TNKS1 and 2 is 10 and 4 nM, respectively and for PARP1and 2–2.2 and 0.1 μM. Therefore, XAV939 was used at concentrations of 0.1 and 1 μM, which are specific for inhibition of TNKS1 and 2. The IC50 of ABT88 towards PARP1 and TNK1 is 5.2 nM, and 15 μM; therefore, it was used at 0.1 μM. Cycloheximide (Sigma-Aldrich) was dissolved in DMSO and used at 100 μg/mL. Ganciclovir (Sigma-Aldrich) was dissolved in PBS and used at 5 μM.
Abt 888
Manufactured by Merck Group
Sourced in United States
ABT-888 is a laboratory product offered by Merck Group. It is a compound used in research settings. The core function of ABT-888 is to serve as a research tool, without further details on its intended use.
Automatically generated - may contain errors
2 protocols using abt 888
1
Pharmacological Inhibition of TNKS, PARP, and Wnt
2
Screening of Small Molecule Inhibitors
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