NHBE or HBE1 cells were transfected at 60–70% confluence with ON-TARGET plus SMARTpool® siRNAs targeted against P2Y2R, CAPN1 or CAPN2 or NON-TARGETing siRNA Pool#1 as control (Dharmacon, Lafayette, CO) using DharmaFECT transfection reagent, according to the manufacturer’s instructions, 72 hrs prior to expermentation. Silencing of DUOX1 or DUOX2 was performed by transfection with the following siRNA reagents: DUOX1: (sense) GCUAUGCAGAUGGCGUGUAtt, (antisense) UACACGCCAUCUGCAUAGCtg; DUOX2: (sense) CGCAGUCAAUGUCUACAUCtt, (antisense) GAUGUAGACAUUGACUGCGtg, or with Silencer Negative Control #1 siRNA (Invitrogen). MTE cells were transfected at 60–70% confluence with pre-designed siRNA targets against DUOX1 (100 nM each) or nON-TARGET (NS) siRNA (Ambion; 200 nM) using DharmaFECT® transfection reagent (Dharmacon) and used for experimentation after 72 hrs (22 (link)). For in vivo siRNA silencing of DUOX1, two DUOX1 siRNA targets in sterile PBS (35 µg/target sequence/mouse) or NS-siRNA (70 µg/mouse) were instilled oropharyngeally in mice under brief isofluorane anesthesia (22 (link)), 48 hrs prior to allergen challenge.
Non targeting sirna pool 1
Manufactured by Horizon Discovery
Sourced in United States
Non-Targeting siRNA Pool #1 is a pre-designed pool of 4 synthetic small interfering RNAs (siRNAs) that do not target any known human, mouse, or rat genes. This product is intended for use as a control in RNA interference (RNAi) experiments.
Automatically generated - may contain errors
Lab products found in correlation
Dharmafect 4 transfection reagent, by Horizon Discovery (2 mentions)
Opti mem serum free medium, by Thermo Fisher Scientific (2 mentions)
On targetplus, by Horizon Discovery (2 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (2 mentions)
Dharmafect transfection reagent, by Horizon Discovery (1 mentions)
Smartpool, by Horizon Discovery (1 mentions)
On target, by Horizon Discovery (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Dharmafect 1, by Horizon Discovery (1 mentions)
Dharmafect 1 transfection reagent, by Horizon Discovery (1 mentions)
Pooled sirna, by Horizon Discovery (1 mentions)
On targetplus smartpool sirna, by Horizon Discovery (1 mentions)
Accell sirna delivery media, by Horizon Discovery (1 mentions)
6 protocols using non targeting sirna pool 1
1
Targeted siRNA Silencing of P2Y2R, CAPN, DUOX
2
Transfecting Breast Cancer Cells
3
Gene Silencing in Primary PAECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary PAECs were transfected with gene-specific siRNA pools targeting BMPR2, JNK1, CAV1 or SMAD9 at a final concentration of 10 nM using DharmaFECT-1 (Dharmacon; Lafayette, CO, USA) in Opti-MEM (Invitrogen; Grand Island, NY, USA) for 6 h followed by growth in EGM™-2 for 48 h. Non-targeting siRNA Pool-1 (siGENOME, Dharmacon, Lafayette, CO, USA) was used as a control.
4
Silencing p65 in Human Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pooled siRNAs designed to specifically target human p65 were purchased from Dharmacon (Lafayette, CO, USA). Cells were transfected with 5 nM ON-TARGETplus SMARTpool siRNA using DharmaFECT 1 transfection reagent (T-2005-01, Dharmacon) according to the manufacturer’s protocol. Non-Targeting siRNA Pool 1 (D-001206-13, Dharmacon) was used as a negative control. Cells were transfected with siRNAs 24 h prior to performing indicated treatments.
5
Investigating IL1F10 siRNA Effects on B Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isolated B cells were incubated with 1 µM Accell SMARTpool Human IL1F10 siRNA (Dharmacon, Lafayette, CO, USA) in Accell siRNA Delivery Media (Dharmacon, Lafayette, CO, USA) containing 100 U/mL penicillin, 100 mg/mL streptomycin, and 50 µg/mL transferrin and bovine Insulin (1:2000). The cells were plated in 200 µL in 96-well U-bottom plates. After 24 h, 20 µL FCS was were added to each well. B cells were cultured for a total of 10 days as mentioned above. On days 0, 4, 7, and 10, supernatants were taken for further analysis, and cells were collected for FACS as well as for qPCR. On days 4 and 7, the cells were washed with PBS and plated in an IMDM medium. As a non-targeting control, Non-Targeting siRNA Pool #1 (Dharmacon, Lafayette, CO, USA) was used at 1 µM.
6
Silencing Pin1 and ESRP1 in Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDA-MB-468 cells were transfected with Pin1 or control siRNA (Table S3 ) using Lipofectamine RNAimax reagent (ThermoFisher Scientific) in Opti-MEM serum-free medium (ThermoFisher Scientific). MCF7 cells were transfected with control (Non-Targeting siRNA Pool #1, Dharmacon; see Key Resources Table ) or ESRP1 siRNA (ON-TARGETplus, Dharmacon; see Key Resources Table ) using DharmaFECT 4 Transfection Reagent (Dharmacon). All analyses were performed 72 hr after transfection.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!