The UV absorbance was measured with a UV-L5S ultraviolet spectrophotometer (Shanghai INESA Scientific Instrument Co., Ltd., Shanghai, China). The sizes and morphologies of nano-SiO2 were observed with a FEI Tecnai G20 transmission electron microscope (FEI, Co., Hillsboro, OR, USA). The particles size distribution of nano-SiO2-HSA was measured with a laser particle size analyzer (Zetasizer Nano, the Malvern Zetasizer, Malvern, UK). The thermo-gravimetric analysis was obtained by heating powdered samples from room temperature to 800 °C under nitrogen atmosphere using a TGA Q500 V20.13 Build 39 thermo-analysis system (TA, Co, New Castle, DE, USA). MALDI-TOF mass data was obtained on a Bruker Autoflex TOF mass spectrometer (Bruker Daltonic, Billerica, MA, USA) operated in linear high internal mode.
Tecnai g20 transmission electron microscope
Manufactured by Thermo Fisher Scientific
Sourced in France, United States
The Tecnai G20 is a transmission electron microscope (TEM) manufactured by Thermo Fisher Scientific. It is designed to perform high-resolution imaging and analysis of a wide range of materials at the nanoscale level. The Tecnai G20 utilizes an electron beam to illuminate and interact with the sample, producing detailed images and data.
Automatically generated - may contain errors
Lab products found in correlation
Dm1000 photonic microscope, by Leica (2 mentions)
Api 20ne, by bioMérieux (2 mentions)
Api zym, by bioMérieux (2 mentions)
Eagle 4k 4k ccd camera, by Thermo Fisher Scientific (2 mentions)
Zetasizer nano, by Malvern Panalytical (1 mentions)
Anaerogen, by bioMérieux (1 mentions)
Cos agar, by bioMérieux (1 mentions)
Jsm 7500f field emission scanning electron microscope, by JEOL (1 mentions)
Sta449f3, by Netzsch (1 mentions)
400 mesh carbon coated copper grid, by Electron Microscopy Sciences (1 mentions)
Eagle 4k x 4k ccd camera, by Thermo Fisher Scientific (1 mentions)
Petri dish, by bioMérieux (1 mentions)
Bbl dryslide, by BD (1 mentions)
Api 50 ch strips, by bioMérieux (1 mentions)
Zetasizer nano zs90 system, by Malvern Panalytical (1 mentions)
400wb avance 3 spectrometer, by Bruker (1 mentions)
Escalab 250xi spectrometer, by Thermo Fisher Scientific (1 mentions)
Jem 2100uhr, by JEOL (1 mentions)
Lambda 35 uv visible spectrometer, by PerkinElmer (1 mentions)
D8 discover diffractometer, by Bruker (1 mentions)
15 protocols using tecnai g20 transmission electron microscope
1
Nano-SiO2 Characterization and Analysis
2
Characterization of Strain SN6 Bacterium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Strain SN6 was isolated at first under microaerophilic conditions (CampyGen) at 37°C for 48 hours on Columbia medium supplemented with 5% sheep's blood (COS) agar (bioMérieux) and we also tested its growth under aerobic and anaerobic conditions generated by AnaeroGen (bioMérieux). The minimum and maximum growth temperature ranges (28°C, 37°C, 45°C, 55°C) were determined as well as maximum salinity levels (0–5, 50–75, 100 g/L NaCl). The ability of the strain to grow on media with different pH was also tested. The colonies appeared on day 3 after culture on Columbia agar, and their diameter was measured. Cell morphology, Gram staining and motility were observed in fresh colonies using a DM1000 photonic microscope (Leica Microsystems, Nanterre, France) with a 40 × objective lens. Sporulation was tested by thermal shock, which consists of exposing the bacterium to a temperature of 60°C for 20 minutes and then watching its growth after 48 hours. Negative staining was carried out with detection Formvar-coated grids placed on a drop of 40 μL of bacterial suspension (after an overnight fixation in glutaraldehyde 2.5%) and incubated at 37°C for 30 minutes, followed by a 10-second incubation in 1% ammonium molybdate. The grids were dried on blotting paper and finally observed using a Tecnai G20 transmission electron microscope (FEI Company, Limeil-Brévannes, France).
3
Characterization of Fe3O4/MIL-88A Composites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to confirm the morphology and structure of the final products, the synthesized magnetic Fe3O4/MIL-88A composites were characterized by means of TEM, field emission scanning electron microscopy (FESEM), and XRD. Specifically, TEM images of magnetic Fe3O4/MIL-88A composites were recorded on a Tecnai-G20 transmission electron microscope (FEI, Hillsboro, OR, USA). FESEM images were recorded on a JSM-7500F Field Emission Scanning Electron Microscope (JEOL, Tokyo, Japan). The XRD spectra were recorded using a powder X-ray Diffractometer (Rigaku RINT 2500, Rigaku Corporation, Tokyo, Japan) with Cu/Kα radiation at 30 mA and 40 kV. TGA was performed in nitrogen atmosphere from 40 to 800 °C with a heating rate of 10 °C/min with a simultaneous thermal analyzer (Netzsch STA 449F3, Ahlden, Germany). Moreover, the magnetic properties of Fe3O4/MIL-88A composites were measured at room temperature on a vibration sample magnetometer VSM7407 (Lake Shore, Westerville, OH, USA).
4
Negative Staining of Bacterial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 3.5 µL drop of bacterial suspension was applied for 30 s to the top of a formvar carbon 400 mesh nickel grid (FCF400-Ni, EMS) that was previously glow discharged. After drying using filter paper, bacteria were immediately stained with 1% ammonium molybdate for 1 sec. Electron micrographs were acquired on a Tecnai G20 transmission electron microscope (FEI Company, Limeil-Brevannes, France) operated at 200 keV.
5
Transmission Electron Microscopy of Purified Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
10-μl drops of purified samples were adsorbed onto freshly glow-discharged 400 mesh carbon-coated copper grids (Electron Microscopy Sciences) for 1 minute and washed in 1–3 drops of (50 μl) 0.1 M and 0.01 M ammonium acetate solutions each. Then, the grids were stained using a freshly filtered 2% solution of uranyl acetate and air-dried after removing the excess stain with filter paper. The stained samples were examined with a Tecnai G20 transmission electron microscope (FEI Company) operating at an acceleration voltage of 200 kV. Electron micrographs were recorded with an Eagle 4k x 4k CCD camera (FEI Company). Defocus levels of 1–2 μm were applied for recording the images. Micrographs acquired for image processing purposes, were recorded at a nominal magnification of 29,000x with a pixel size of 3.07 Å per pixel.
6
Morphological and Biochemical Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Morphologic characterization was first performed by observation of Gram staining and motility of the fresh sample. Negative staining was then performed using bacteria fixed with 2.5% glutaraldehyde, deposited on carbon formvar film and then incubated for 1 second on ammonium molybdate 1%, dried on blotting paper and finally observed using a TECNAI G20 transmission electron microscope (FEI, Limeil-Brevannes, France) at an operating voltage of 200 keV. Biochemical features, such as oxidase, catalase, API 50CH, 20A and ZYM strips (bioMérieux, Marcy l'Étoile, France), were investigated according to the manufacturer's instructions. Cellular fatty acids were analysed from two samples prepared with approximately 10 mg of bacterial biomass each collected from several culture plates. Fatty acid methyl esters were prepared as described [14] and gas chromatography mass spectrometry (GC/MS) analyses were carried out as described previously [15] (link).
7
Comprehensive Characterization of Bacterial Strain
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gram staining and motility were observed from fresh colonies between blades and slats using a DM1000 photonic microscope (Leica Microsystems, Nanterre, France) with a 40 × objective lens [16] (link). Spore formation was determined by thermal shock (80°C for 20 minutes) and observed under a microscope. Negative staining was carried out with detection formvar-coated grids placed on a drop of 40 μL of bacterial suspension and incubated at 37°C for 30 minutes, followed by a 10-second incubation in 1% ammonium molybdate. The grids were dried on blotting paper and then observed with a Tecnai G20 transmission electron microscope (FEI Company, Limeil-Brévannes, France). We studied the biochemical characteristics of this strain using API 20NE, API ZYM and API 50CH strips according to the manufacturer's instructions (bioMérieux). Oxidase and catalase reactions were determined using a BBL DrySlide (Becton, Le Pont de Claix, France) according to the manufacturer's instructions. The antimicrobial activity test was performed using the disc diffusion method (i2a, Montpellier, France) [17] (link) on Mueller-Hinton agar in a petri dish (bioMérieux).
8
Electron Microscopy Analysis of Pb Stress
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses were conducted at the Cairo University Research Park (CURP), Faculty of Agriculture, Cairo University, Giza, Egypt, to determine potential Pb stress cells exhibit any structural changes and where metal accumulated. For SEM, a drop of the suspension from the bacterial culture, both with and without Pb, was placed on an aluminum stub and subjected to the procedures outlined by Khan et al. (2016) (link). The gold film was then applied to samples using a sputter coater (Denton, Desk V HP) and viewed under a SEM (Nova NanoSEM 450). The TEM for the Pb-treated and untreated bacteria was carried out using a Tecnai G 20 transmission electron microscope (FEI, Limeil-Brevannes, France) SA 9900 at 200 kV (Burghardt and Droleskey, 2006 (link)). Images were captured using a high-resolution digital CCD camera and Olympus Soft Imaging System’s (Germany) iTEM image processing software.
9
Characterization of Nanoparticles and Biomolecules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The prepared AuNP, magnetic nanoparticle (MNP), Aβ42Ms, and Aβ42Os were confirmed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), respectively. In short, a small drop of samples (5 μL) was deposited onto a copper grid, and the excess liquid was removed by blotting using a filter paper, thus leaving a thin film of the solution on the grid. Subsequently, Tecnai G20 transmission electron microscope (FEI Ltd., USA) and Phenom Pharos scanning electron microscope (SEM) (Phenom Ltd., NLD) were used to characterize the morphology of the abovementioned samples. The particle size distribution and Zeta-potential of all nanoparticles were measured via dynamic light scattering (photon correlation spectroscopy) on a Zetasizer Nano-ZS90 system (Malvern Instruments, Worcestershire, UK).
10
Retinal Tissue Preparation for TEM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For TEM, the retinal tissues were fixed in 2.5% glutaraldehyde and 2% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4). After 6 h of fixation at 4 °C, the retinas were cut around 2 mm from the region of the optic nerve head and further trimmed into 1–2 mm pieces. The 1–2 mm pieces were again fixed in 1% osmium tetraoxide, dehydrated, and embedded in araldite CY 212. The semithin sections (500 nm thick) were examined under a light microscope to select the areas of interest after staining with 0.5% toluidine blue. Thin sections (70 nm) were cut using an ultramicrotome, contrasted with uranyl acetate and lead citrate and viewed under a TECNAI G20 transmission electron microscope (FEI, Eindhoven, the Netherlands).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!