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633 phalloidin

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633-phalloidin is a fluorescent dye used in microscopy techniques to label and visualize actin filaments within cells. It binds specifically to filamentous actin (F-actin) with high affinity. 633-phalloidin can be used in various applications such as cell biology research, cytoskeleton studies, and imaging of the cellular architecture.

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Lab products found in correlation

Lsm 780 microscope, by Zeiss (2 mentions) Anti vimentin, by Merck Group (2 mentions) Anti fibronectin, by Merck Group (2 mentions) Anti α sma, by Merck Group (2 mentions) Cy3 conjugated clone 1a4, by Merck Group (2 mentions)

Spelling variants of this product

Phalloidin-Atto 633 (9 citations) Atto 633-conjugated phalloidin (2 citations)

2 protocols using 633 phalloidin

1

Immunofluorescence Staining of HNSCC

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Human head and neck squamous cell carcinoma samples were obtained from the Royal Marsden Hospital after informed consent through the project CCR2924 (Approved by the London/Chelsea NHS REC with code REC: 06/Q0403/125). All material was handled according to relevant ethical regulations by the Human Tissue Act. After excision the tumours were flash-frozen. Sections were stained as previously described 46 (link). Briefly, fresh frozen sections were fixed in 4% paraformaldehyde, permeabilized in 0.2% Triton X-100, and stained with the following antibodies: anti-fibronectin (dilution 1/500, Sigma, F3648), anti-vimentin (dilution 1/100, Sigma,V2258), anti-TFAP2C (dilution 1:100, Abacm#218107), anti-αSMA (dilution 1/200, Cy3-conjugated clone#1a4, Sigma, C6198) and 633-phalloidin (dilution 1:500, Sigma, 68825). Sections were mounted using MOWIOL reagent and imaged using a Zeiss LSM 780 microscope.
Park D., Wershof E., Boeing S., Labernadie A., Jenkins R.P., George S., Trepat X., Bates P.A, & Sahai E. (2019). Extracellular matrix anisotropy is determined by TFAP2C-dependent regulation of cell collisions. Nature materials, 19(2), 227-238.
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2

Immunofluorescence Staining of HNSCC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human head and neck squamous cell carcinoma samples were obtained from the Royal Marsden Hospital after informed consent through the project CCR2924 (Approved by the London/Chelsea NHS REC with code REC: 06/Q0403/125). All material was handled according to relevant ethical regulations by the Human Tissue Act. After excision the tumours were flash-frozen. Sections were stained as previously described 46 (link). Briefly, fresh frozen sections were fixed in 4% paraformaldehyde, permeabilized in 0.2% Triton X-100, and stained with the following antibodies: anti-fibronectin (dilution 1/500, Sigma, F3648), anti-vimentin (dilution 1/100, Sigma,V2258), anti-TFAP2C (dilution 1:100, Abacm#218107), anti-αSMA (dilution 1/200, Cy3-conjugated clone#1a4, Sigma, C6198) and 633-phalloidin (dilution 1:500, Sigma, 68825). Sections were mounted using MOWIOL reagent and imaged using a Zeiss LSM 780 microscope.
Park D., Wershof E., Boeing S., Labernadie A., Jenkins R.P., George S., Trepat X., Bates P.A, & Sahai E. (2019). Extracellular matrix anisotropy is determined by TFAP2C-dependent regulation of cell collisions. Nature materials, 19(2), 227-238.
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