Pd123319

Manufactured by Merck Group

Sourced in United States, Germany, United Kingdom, Sweden, Australia

PD123319 is a laboratory research tool that functions as a selective antagonist for the angiotensin II type 1 (AT1) receptor. It is commonly used in scientific research to study the role of the AT1 receptor in various biological processes.

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Lab products found in correlation

Losartan, by Merck Group (18 mentions) Ang 2, by Merck Group (11 mentions) Cgp42112a, by Merck Group (5 mentions) Angiotensin 2, by Merck Group (5 mentions) Ang 1 7, by Merck Group (4 mentions) Captopril, by Merck Group (3 mentions) Telmisartan, by Merck Group (3 mentions) Irbesartan, by Merck Group (3 mentions) Candesartan, by Merck Group (3 mentions) Ema401, by Novartis (2 mentions) Lipopolysaccharide, by Merck Group (2 mentions) Norepinephrine, by Merck Group (2 mentions) Ly294002, by Merck Group (2 mentions) Wild type c57bl 6j mice, by Jackson ImmunoResearch (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Angii, by Bachem (2 mentions) Apocynin, by Merck Group (2 mentions) Gw9662, by Merck Group (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)

55 protocols using pd123319

Prorenin Signaling Pathway in Renal Cells

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NRK-52E cells (ATCC, Manassas, VA) were cultured and prepared as previously described17 (link). Cells were treated with human recombinant prorenin (Cayman Chemical, Ann Arbor, MI) at 0, 10, 20, 40, 80, and 100 pmol/L for 24 h or 100 pmol/L for 0, 6, 12, 24, and 48 h after preincubation with the Ang II type 1 receptor blocker losartan (10 μmol/L) (Sigma, San Francisco, CA,) or the Ang II type 2 receptor blocker PD123319 (10 μmol/L) (Sigma, San Francisco, CA) for 1 h.
The cells were treated with bafilomycin A1 (1 nmol/L, Sigma-Aldrich), losartan (10 μmol/L), and PD123319 (10 μmol/L) for 1 h before prorenin (100 pmol/L) incubation for 48 h to evaluate the effects of the V-ATPase inhibitor bafilomycin A1 on prorenin-induced FN and α-SMA expression in NRK52E cells. Cells were harvested and used in MTT (3-(4, 5-dimethyl (thiazol-2-yl)-2, 5-diphenyltetrazolium bromide) cell viability, immunoblotting, real-time polymerase chain reaction (PCR), and immunofluorescence assays.

Liu Y., Zuo S., Li X., Fan J., Cao X., Yu X, & Yang Q. (2016). Interaction between V-ATPase B2 and (Pro) renin Receptors in Promoting the progression of Renal Tubulointerstitial Fibrosis. Scientific Reports, 6, 25035.

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Angiotensin-(1-9) Attenuates MCT-Induced Pulmonary Hypertension

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Experiment was conducted with five groups (n=8 for each group).
Group 1 was control rats received saline. Group 2 was MCT-induced PH rats. Adult male Sprague-Dawley rats weighing 200–220 g received a single subcutaneous injection of 50 mg/kg MCT (Sigma-Aldrich Co., St Louis, MO) to induce PH. Group 3 was MCT-induced PH rats pretreated with Ang-(1-9) (Bachem, Switzerland). Ang-(1-9) was infused 3 days before MCT injection for 24 days at a dose of 576 µg/kg/day via a mini-osmotic pumps (Alzet 2004, Cupertino, CA) implanted subcutaneously between the scapulars [10 (link)]. Three days after the start of Ang-(1-9) infusion, MCT was injected. Groups 4 and 5 were MCT-induced PH rats pretreated with Ang-(1-9) in the presence of AT₂R or Mas R antagonist. Either PD 123,319 (576 µg/kg/day; Sigma-Aldrich Co.) [3 (link)] or A779 (576 µg/kg/day; Bachem) [10 (link)] was infused via miniosmotic pumps 2 days before the start of Ang-(1-9) infusion, and Ang-(1-9) was then co-administered 2 days later. Three days after the start of Ang-(1-9) infusion, MCT was injected. All rats were sacrificed 21 days after MCT injection.

Cha S.A., Park B.M, & Kim S.H. (2018). Angiotensin-(1-9) ameliorates pulmonary arterial hypertension via angiotensin type II receptor. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 22(4), 447-456.

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Valsartan-Mediated Shc Phosphorylation

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TSA, valsartan, PD123319, and Ang II were obtained from Sigma-Aldrich (St. Louis, MO, USA). The following antibodies were used: phospho-ser36-p66shc (Calbiochem, La Jolla, CA, USA), Shc (BD Biosciences, Franklin Lakes, NJ, USA), and β-actin (Santa Cruz Biotechnology, CA, USA). Lipofectamine 2000 was purchased from Life Technologies (Carlsbad, CA, USA) and a chemiluminescence kit from Amersham Pharmacia Biotech (Piscataway, NJ, USA).

Kang G., Lee Y.R., Joo H.K., Park M.S., Kim C.S., Choi S, & Jeon B.H. (2015). Trichostatin A Modulates Angiotensin II-induced Vasoconstriction and Blood Pressure Via Inhibition of p66shc Activation. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 19(5), 467-472.

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Probing AT2R Signaling Pathways

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TGF-β1 and CGP42112A were obtained from Sigma (St. Louis, MO, USA). The AT₂R antagonists, PD123319 and PD123177, and cycloheximide were also obtained from Sigma. Antibodies against TGF-βRII and E-cadherin were rabbit anti-human polyclonal antibodies. The antibody against α-SMA was mouse monoclonal antibody. The antibody against AT₂R was an affinity purified goat polyclonal antibody. All of the antibodies were obtained from Santa Cruz Biotechnology, Inc (Dallas, TX, USA). All other chemicals for various buffers were of the highest purity available and purchased exclusively from Sigma or Gibco (Gibco, Grand Island, NY, USA).

Guo H.L., Liao X.H., Liu Q, & Zhang L. (2016). Angiotensin II Type 2 Receptor Decreases Transforming Growth Factor-β Type II Receptor Expression and Function in Human Renal Proximal Tubule Cells. PLoS ONE, 11(2), e0148696.

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Pharmacological Modulation of Angiotensin and Calcium Signaling

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Angiotensin II (Peptide Institute, Osaka, Japan), losartan (Wako Pure Chemical Industries, Osaka, Japan), PD123,319 (Sigma-Aldrich, St. Louis, MO, USA), angiotensin I (Peptide Institute, Osaka, Japan), captopril (Sigma-Aldrich, St. Louis, MO, USA), chymostatin (Sigma-Aldrich, St. Louis, MO, USA) and nickel(II) chloride hexahydrate (Sigma-Aldrich, St. Louis, MO, USA) were dissolved in water. Carvedilol (Tokyo Chemical Industry, Tokyo, Japan), xestospongin C (Wako Pure Chemical Industries, Osaka, Japan), SEA0400 (synthesized in our faculty according to the reported method [75 (link)]), 2-APB (Sigma-Aldrich, St. Louis, MO, USA) and ryanodine (Wako Pure Chemical Industries, Osaka, Japan) were dissolved in dimethyl sulfoxide (DMSO). They were added to the organ bath to obtain the desired final concentration.

Tanaka Y., Obata K., Ohmori T., Ishiwata K., Abe M., Hamaguchi S., Namekata I, & Tanaka H. (2019). Angiotensin II Induces Automatic Activity of the Isolated Guinea Pig Pulmonary Vein Myocardium through Activation of the IP3 Receptor and the Na+-Ca2+ Exchanger. International Journal of Molecular Sciences, 20(7), 1768.

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NCC Phosphorylation in Hypertension

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Angiotensin II, hydrochlorothiazide (HCTZ), CGP42112A, losartan and PD123319 were purchased from Sigma (St Louis, MO). The NCC antibody was a gift from Robert Hoover at Emory University, whereas antibody of phosphorylated NCC at threonine 53 (Thr⁵³) was kindly provided by David H. Ellison at Oregon Health & Science University. The data are shown as mean ± SEM. We used the paired t test or one-way ANOVA test to determine the statistical significance.

Wu P., Gao Z.X., Duan X.P., Su X.T., Wang M.X., Lin D.H., Gu R, & Wang W.H. (2018). AT2R-mediated regulation of Na-Cl cotransporter (NCC) and renal K excretion depends on the K channel, Kir4.1. Hypertension (Dallas, Tex. : 1979), 71(4), 622-630.

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Angiotensin Receptor Binding Assay

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Ang‐[1‐8], Ang‐[1‐10], lisinopril, losartan, PD123319 were purchased from Sigma. Isoflurane was purchased from Cristália Ltda and Patterson Veterinary, Ketoprofen was purchased from Mundo Animal, ketamine (80 mg/kg) and xylazine (7 mg/kg). Penicillin, streptomycin, and dihydrostreptomycin were purchased from Fort Dodge Animal Health. Mca‐Ala‐Pro‐Lys(Dnp)‐OH was purchased from Enzo Life Science. MLN‐4760 was purchased from EMD Milllipore. Pierce BCA protein assay kit was purchased from ThermoFischer. ¹²⁵I‐SI‐Ang‐[1‐8] was radiolabeled by Robert C. Speth, Ph.D. (Peptide Radioiodination Shared Resource, Georgetown University).

De Souza A.M., Linares A., Speth R.C., Campos G.V., Ji H., Chianca D J.r., Sandberg K, & De Menezes R.C. (2020). Severe food restriction activates the central renin angiotensin system. Physiological Reports, 8(1), e14338.

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Evaluating Oxidative Stress in PC12 Cells

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PC12 cells were obtained from Pasteur Institute (Tehran, Iran). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT), 2’,7’-dichlorofluorescein diacetate (DCF-DA), captopril, telmisartan, losartan, PD-123319, dimethyl sulfoxide, and D-glucose were purchased from Sigma-Aldrich company (St. Louis, MO, USA). DMEM, fetal bovine serum, trypsin, penicillin, and streptomycin were obtained from Gibco BRL Life Technologies (Grand Island, NY, USA) and annexin V-FITC & PI kit was purchased from BioVision (Mountain View, CA, USA).

Shahveisi K., Mousavi S.H., Hosseini M., Rad A.K., Jalali S.A., Rajaei Z., Sadeghnia H.R, & Hadjzadeh M.A. (2014). The role of local renin-angiotensin system on high glucose-induced cell toxicity, apoptosis and reactive oxygen species production in PC12 cells. Iranian Journal of Basic Medical Sciences, 17(8), 613-621.

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Angiotensin II Receptor Antagonists Effects

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Cells were seeded in cell culture dishes and incubated at 37°C/5% CO₂ until becoming confluent. Then, these cells were pre-treated (30 minutes) with either AT1 receptor antagonist (Losartan: 10⁻⁵ M) or AT2 receptor antagonist (PD123319: 10⁻⁵ M) followed by Ang II treatment (10⁻⁷ M) according to the treatment scheme: Group 1 – control; Group 2 – cells only treated with Ang II; Group 3 – cells pre-treated (30 minutes) with Losartan and then treated with Ang II; Group 4 – cells pre-treated (30 minutes) with PD123319 and then treated with Ang II. Ang II, Losartan and PD123319 were obtained from Sigma Chemicals (St Louis, US).

Azevedo H., Fujita A., Bando S.Y., Iamashita P, & Moreira-Filho C.A. (2014). Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression. PLoS ONE, 9(11), e110934.

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Angiotensin II Receptor Antagonists Evaluation

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Agonists, antagonists, and the drugs AngII, losartan, PD123319, and TMZ were purchased from Sigma, UK. The highly selective AT₂R antagonist EMA401 was kindly supplied by Novartis, Switzerland, and purchased from Biorbyt, UK.

Perryman R., Renziehausen A., Shaye H., Kostagianni A.D., Tsiailanis A.D., Thorne T., Chatziathanasiadou M.V., Sivolapenko G.B., El Mubarak M.A., Han G.W., Zarzycka B., Katritch V., Lebon G., Lo Nigro C., Lattanzio L., Morse S.V., Choi J.J., O’Neill K., Kanaki Z., Klinakis A., Crook T., Cherezov V., Tzakos A.G, & Syed N. (2022). Inhibition of the angiotensin II type 2 receptor AT2R is a novel therapeutic strategy for glioblastoma. Proceedings of the National Academy of Sciences of the United States of America, 119(32), e2116289119.

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