Rosa26ai9

Syn1-Cre (Stock No: 003966), GFAP-Cre (Stock No: 024098), Thy1-GFP (Stock No: 007788), Rosa26^Ai9/+ (Stock No: 007909), Fmr1 knockout (Stock No: 003025), CD68-EGFP (Stock No: 026827) and Cx3cr1^CreER/+ (Stock No: 021160) mouse strains were obtained from the Jackson Laboratory. The Pten^+/− strain was from the National Cancer Institute. All mice were maintained at 22 °C on a 12-h/12-h light/dark cycle with ad libitum access to water and food. Animal procedures were approved by the Scripps Florida Institutional Animal Care and Use Committee.

All mouse experiments were carried out with strict observance of protocols and guidelines approved by the University of Bonn Animal Care and Use Committee, Federal Government of Germany and European Union legislation. The protocols were approved by the Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen (Permit Number: 84-02.02.2016.A238). The mice were housed under controlled light (12 h light/dark cycle at an ambient temperature of 22°C). Water and mice chow were available ad libitum.
Cxcr4^{CreER(T2)-IRES-eGFP}(Cxcr4^CreER) mice were provided by Ralf Stumm (Werner et al., 2020 (link)). Rosa26^Ai9 (stock #007909; Madisen et al., 2010 (link)), Dat^tTA (stock #027178; Chen et al., 2015 (link)), and Ai82D (lgs7^TITLGFP; stock #023532; Madisen et al., 2015 (link)) mice were purchased from The Jackson Laboratory. For inducible fate-mapping studies, Cxcr4^CreER/+; Rosa26^Ai9/Ai9 males were bred with CD1 wild-type females (Charles River) to generate Cxcr4^CreER/+; Rosa26^Ai9/+ progeny. For intersectional fate mapping, Cxcr4^CreER/+; Dat^tTA/+; lgs7^{TITLGFP/TITLGFP} males were bred with CD1 wild-type females (Charles River) to generate Cxcr4^CreER/+; Dat^tTA/+; lgs7^TITLGFP progeny. Mice of either sex were used for experiments.