For this study, we used Quantulus1220, two Tri-Carbs 3110 (#1 and #2), Tri-Carb 5110, and Quantulus GCT6220 instruments (all from PerkinElmer). We also used 20-mL LSC plastic vials (PerkinElmer) for LSC analysis, polyethylene bottles of different volume (Fisher Scientific), and a Brinkman bottletop dispenser with capacity from 5 mL to 25 mL (Brinkman Instruments) for cocktail dispensing. Additional supplies included four electronic pipettes with total volume range from 5 μL to 5 mL (Eppendorf) and a high-precision (0.0001 g accuracy) analytical balance (Mettler-Toledo).
Electronic pipettes
Manufactured by Eppendorf
Electronic pipettes are laboratory instruments used to accurately measure and transfer precise volumes of liquids. They provide consistent and reliable liquid handling, offering precise and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
Tri carb 5110, by PerkinElmer (1 mentions)
Quantulus 1220, by PerkinElmer (1 mentions)
20 ml lsc plastic vials, by PerkinElmer (1 mentions)
Cfx384, by Bio-Rad (1 mentions)
High precision analytical balance, by Mettler Toledo (1 mentions)
Polypropylene bottles, by Thermo Fisher Scientific (1 mentions)
3 protocols using electronic pipettes
1
Radiometric Counting Instrument Protocols
2
Quantitative SARS-CoV-2 Variant Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AS RT-qPCR was performed using the Taqman Virus 1-Step master mix (Thermofisher #4444434) with technical duplicates, at a final volume of 10 µL, according to the manufacturer's recommendations. A single reverse primer and probe were used with each allele-specific forward primer (Table 2 ). The final concentration of the AS RT-qPCR primers was 500 nM, probe at 200 nM, with 1 µL of template. No template controls were included for each assay and none of them amplified. The reactions are setup using electronic pipettes (Eppendorf) and performed on a Bio-Rad CFX384 real-time PCR instrument under the following conditions, 5 min at 50 °C and 20 s at 95 °C, followed by 45 cycles of 3 s at 95 °C and 30 s at 60 °C.Table 2
Primers and probes in each assay.
| 493–498 | WT 493–498 | F-WT-493–498 | R-Gen-493–498 | Ps-Gen-493–498 |
| Omicron 493-498 | F-Om-493–498 |
3
Radioactive Isotope Measurement Protocols
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!