Log In Sign up
The largest database of trusted experimental protocols

Nanojet

Manufactured by Chemyx
Visit Supplier
Sourced in United States

The NanoJet is a precision instrument designed for the injection and delivery of nanoliter-scale volumes of fluids. It features a high-precision syringe pump and a specialized injection mechanism to ensure accurate and repeatable fluid dispensing. The NanoJet is a versatile tool suitable for a range of applications requiring the controlled manipulation of small-volume samples.

Automatically generated - may contain errors

Lab products found in correlation

Ttl180 a, by Thorlabs (1 mentions) Dg10 1500, by Thorlabs (1 mentions) K3fe cn 6, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Nanojet Injector Module (5 citations) NanoJet syringe pump (2 citations)

6 protocols using nanojet

1

In Vivo Delivery of MINDS into Mouse Brains

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vivo injection of MINDS into the brains of live mice was performed using a controlled stereotaxic injection method similar to virus injection described above. MINDS were dispersed in sterile Hank’s Buffer with 20 mM Hepes solution (HHBS; AAT Bioquest, Sunnyvale, CA; containing 140 mg L−1 anhydrous CaCl2) before injection. Mice were anaesthetized as previously described, and scalp incision and craniotomy were performed at the corresponding coordinates for M2, HIP or VTA. 2.5 μL of MINDS solution (1.8 mg mL−1) was injected into targeted brain region using NanoJet (Chemyx, Inc., Stafford, TX) with a 33-gauge needle (Hamilton Company, Inc., Reno, NV) facing the ventrolateral side at 0.1 μL min−1. The same amount of HHBS instead of MINDS was injected for MINDS (−) controls. After injection of MINDS, the syringe needle was allowed to stay inside the brain for 2 min before withdrawal. The incised scalp skin was sealed as described above.
Wu X., Jiang Y., Rommelfanger N.J., Yang F., Zhou Q., Yin R., Liu J., Cai S., Ren W., Shin A., Ong K.S., Pu K, & Hong G. (2022). Midbrain stimulation in freely behaving mice by photothermal transducers actuated via widefield near-infrared II light. Nature biomedical engineering, 6(6), 754-770.
+ Open protocol
+ Expand
2

Surface Tension Measurement of Lipid-Buffer Interface

Check if the same lab product or an alternative is used in the 5 most similar protocols
A custom pendant droplet tensiometer was created to measure the surface tension of the TAG-buffer interface upon the addition of PLs. The droplet volume was controlled by a syringe pump (NanoJet, Chemyx) with a 1 mL TAG-filled syringe (1001 LT SYR, Hamilton) connected via a PEEK tubing to a 30-gauge blunt-tipped needle (930050–90BTE, Metcal) within a polystyrene cuvette (759076D, BrandTech). The droplet was imaged with CMOS camera (MU233-FL, AmScope), tube lens (TTL180-A, ThorLabs), and 4x air objective (PlanN, Olympus) after illumination with a diffused (DG10–1500, Thorlabs) desktop LED lamp (DLST01-S, Newhouse). Images were analyzed and the surface tension was extracted with FIJI and OpenDrop.31 (link),32 (link) FIJI was used to convert the
Gandhi S.A., Parveen S., Alduhailan M., Tripathi R., Junedi N., Saqallah M., Sanders M.A., Hoffmann P.M., Truex K., Granneman J.G, & Kelly C.V. (2023). Methods for making and observing model lipid droplets. bioRxiv.
+ Open protocol
+ Expand
3

Precise Inoculation of Xylella fastidiosa in Plants

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The inoculation of X. fastidiosa was performed by microinjection applied to the main stem as described previously (Baró et al., 2021 (link); Moll et al., 2022 (link)). Briefly, a pathogen suspension, at 108 CFU/ml (OD600 ≅ 0.3) confirmed by plate counting, was prepared in phosphate buffered saline (PBS), pelleted (10 min at 13,000 rpm) and resuspended in PD2 broth 1x, to avoid cell aggregation inside the syringe during injection and to ensure cell viability. Inoculations were performed using a high precision microinjector (NanoJet, Chemyx, Stafford, TX, USA) provided with a Hamilton 250 µl syringe including a thin needle with bevel tip (Bondaluz, Switzerland). The needle end was introduced into approximately one half the plant stem diameter to directly access the vascular system, as described previously. Three inoculations of X. fastidiosa suspension of 10 µl each (30 µl of total inoculum/plant, 3x106 CFU/plant) were applied at the same side of the stem in a section of 3 cm at around 10 cm above the soil level (Figure 1).
Inoculated plants were cultivated in a Biosafety level II+ quarantine greenhouse authorized by the Plant Health Services, according to EPPO recommended containment conditions (EPPO, 2006 (link)), taking into consideration the quarantine status of X. fastidiosa in the EU (EFSA PLH Panel et al., 2018 (link)).
Baró A., Saldarelli P., Saponari M., Montesinos E, & Montesinos L. (2022). Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide. Frontiers in Plant Science, 13, 1061463.
+ Open protocol
+ Expand
4

Neonatal Optogenetic Manipulation of Motor Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neonatal pups (P0-P1) were anesthetized via hypothermia on a wet towel on ice and placed on an ice-cold stereotax. The scalp was rubbed with 70% ethanol before and after the injection procedure. Pups were injected with 0.5 μL of AAV1-Syn-ChrimsonR-tdTomato (1 x 1012 vg/mL) in each hemisphere of the motor cortex using a 34 gauge, 0.375” needle with a 12° tip in a Hamilton syringe attached to syringe pump (NanoJet, Chemyx Inc). The narrow gauge and sharp tip of the needle allowed it to penetrate directly into the cortex through the scalp and skull. An injection rate of 0.75 μL/min was used, and 30 seconds were allowed before removing the needle after the injection completed. Pups recovered on a heat pad until pink and wriggling before being rubbed with bedding and feces from their home cage and returned to the mother.
Ceto S., Sekiguchi K.J., Takashima Y., Nimmerjahn A, & Tuszynski M.H. (2020). Neural stem cell grafts form extensive synaptic networks that integrate with host circuits after spinal cord injury. Cell stem cell, 27(3), 430-440.e5.
+ Open protocol
+ Expand
5

Fabrication and Operation of Microfluidic Devices for GUV Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The master wafers and poly(dimethylsiloxane) chips were fabricated as previously described25 ,26 (link) and detailed in the Supporting Information. In short, the device comprised a top pressure layer and a bottom fluid layer 20 μm in height and was finally bonded to a cover glass (#1.5, Menzel-Gläser). All the solutions were infused into the fluid layer using a syringe pump (NanoJet, Chemyx). The channels were coated with 4% (w/v) bovine serum albumin (BSA) (Sigma-Aldrich) in phosphatebuffered saline buffer (pH 7.4, 1×, Gibco) before GUVs were introduced and trapped. Afterward, the pressure valves were activated through a home-built pressure controller to 2 bar, whereas 20 nM calcein, 10−35 nM Arg9-Atto488, TAT(RRRQRRKKRG)-Atto488, TAT-Alexa488 (PSL Peptide Specialty Laboratories; all labels attached to the N-terminal arginines and purified as trifluoroacetate salts), or 50 nM FAM-Adp8, FAM-(AdpOMe)8, FAM-(AdpNMe2)8 (synthesized with fluorenylmethyloxycarbonyl (Fmoc) chemistry, as published in ref 32 (link)) in the hosting buffer was flushed into the channels. The integrated valves were opened and closed again for FCS measurements, except for the experimental series under flow. For the photo-oxidation reaction, a 20 mM stock solution of potassium ferricyanide (K3Fe(CN)6, Acros) was diluted 1:100 into the peptide solution to reach a final concentration of 200 μM.
Lin C.C., Bachmann M., Bachler S., Venkatesan K, & Dittrich P.S. (2018). Tunable Membrane Potential Reconstituted in Giant Vesicles Promotes Permeation of Cationic Peptides at Nanomolar Concentrations. ACS applied materials & interfaces, 10(49), 41909-41916.
+ Open protocol
+ Expand
6

In Vivo Delivery of MINDS into Mouse Brains

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vivo injection of MINDS into the brains of live mice was performed using a controlled stereotaxic injection method similar to virus injection described above. MINDS were dispersed in sterile Hank’s Buffer with 20 mM Hepes solution (HHBS; AAT Bioquest, Sunnyvale, CA; containing 140 mg L−1 anhydrous CaCl2) before injection. Mice were anaesthetized as previously described, and scalp incision and craniotomy were performed at the corresponding coordinates for M2, HIP or VTA. 2.5 μL of MINDS solution (1.8 mg mL−1) was injected into targeted brain region using NanoJet (Chemyx, Inc., Stafford, TX) with a 33-gauge needle (Hamilton Company, Inc., Reno, NV) facing the ventrolateral side at 0.1 μL min−1. The same amount of HHBS instead of MINDS was injected for MINDS (−) controls. After injection of MINDS, the syringe needle was allowed to stay inside the brain for 2 min before withdrawal. The incised scalp skin was sealed as described above.
Wu X., Jiang Y., Rommelfanger N.J., Yang F., Zhou Q., Yin R., Liu J., Cai S., Ren W., Shin A., Ong K.S., Pu K, & Hong G. (2022). Midbrain stimulation in freely behaving mice by photothermal transducers actuated via widefield near-infrared II light. Nature biomedical engineering, 6(6), 754-770.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!