Antibodies specific for CD16/CD32 (93), CD45 (30-F11), F4/80 (BM8), CD11c (N418), CD11b (M1/70), Ly6G (1A8-Ly6g), B220 (RA3-6B2), cKit (2B8), MHC-II (M5/114.15.2), IgG2a (eBr2a), CD3 (145-2C11), CD49b (DX5) from eBioscience; Ly6C (HK1.4), Ly6G (1A8), CD64 (X54-5/7.1) from BioLegend; and E-cadherin (36/E-Cadherin) from BD Biosciences were used. Data were acquired using an iCyt Synergy and analyzed with FlowJo (Tree Star); negative gating controls shown are FMOs (grey histograms); MFI refers to mean fluorescence intensity. Sorted cells had an average purity of 96% and were recovered for at least 30 min at 37°C in media with 20% FBS. For intracellular cytokine staining, cells were incubated in Brefeldin A (3 μg/ml) for 4 h at 37°C in 7% CO2, fixed and permeabilized (BD Cytofix/Cytoperm), and stained with either NOS2 (CXNFT; eBioscience) or IFNγ (XMG1.2; BioLegend) antibodies.
Nos2 cxnft
Manufactured by Thermo Fisher Scientific
The NOS2 (CXNFT) is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed to detect and quantify the expression of the inducible nitric oxide synthase (iNOS) gene in biological samples. The NOS2 (CXNFT) provides a reliable and efficient tool for researchers studying nitric oxide signaling and its role in various biological processes.
Automatically generated - may contain errors
2 protocols using nos2 cxnft
1
Immune Cell Profiling by Flow Cytometry
2
Multiparameter immune cell profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antibodies used for cell surface staining were obtained from eBioscience or BioLegend, anti-Ly6G (1A8), anti-Ly6C (HK1.4), anti-Gr1 (RB6-8C5), anti-CD11b (M1/70), anti CD19 (eBio1D3), anti-CD3 (17A2), anti-CD39 (24DMS1) anti-PD-L1 (MIH5), anti-CD80 (16-10A1), anti-CD8 (53-6.7), anti-CD43 (1B11), anti-NK1.1(PK136) anti-Ter119 (Ter-119) and FC block anti-mouse CD16/CD32 [91 (link)]. Intracellular Granzym B (monoclonal anti-human granzyme B (GB11) antibody Invitrogen) staining was performed as previously described [96 (link)]. Intracellular expression of Ki67 (SolA15), Arg1 (R&D Systems) and NOS2 (CXNFT) was detected using a Foxp3 staining kit (eBioscience). Dead cells for cell-surface and intracellular staining were excluded with use of Fixable Viability Dye eFluor 780 (FVD) (eBioscience). Data were acquired on a LSR II flow cytometer (Becton–Dickinson) from 300,000 to 500,000 lymphocyte-gated events per sample. Analyses were done using FlowJo (Treestar).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!