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2 protocols using 1 3 3 tetraethoxypropane

1

Quantification of Lipid Peroxidation Markers

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The materials used in this research are as follows: 1,3,3-tetraethoxypropane (Sigma, CAS number 122-31-6), 2-thiobarbituric acid (Merck, CAS number 504-17-6), 5,5′-dithiobis(2-nitrobenzoic acid) (Sigma, CAS number 69-78-3), acetic acid (Merck, CAS number 64-19-7), acetonitrile (Merck, CAS number 75-05-8), EGTA (Sigma, CAS number 67-42-5), FeCl3 (Sigma, CAS number 7705-08-0), FeSO4 (Sigma, CAS number 7720-78-7), KCl (Merck, CAS number 7447-40-7), methanol (Merck, CAS number 67-56-1), MgCl2 (Merck, CAS number 7786-30-3), Na2EDTA (Merck, CAS number 6381-92-6), NaCl (Sigma, CAS number 7647-14-5), ouabain (Merck, CAS number 11018-89-6), sodium citrate (Merck, CAS number 6132-04-3), sodium dodecyl sulfate (Merck, CAS number 151-21-3), tetrahydrofuran (Merck, CAS number 109-99-9), TPTZ (Sigma, CAS number 3682-35-7), trichloroacetic acid (Merck, CAS number 76-03-9), and Tris hydrochloride (Sigma, CAS number 1185-53-1).
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2

Comprehensive Oxidative Stress Analysis

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Thiobarbituric acid (TBA), sodium dodecyl sulfate (SDS), glacial acetic acid, N-butanol, pyridine, 1,3,3-tetraethoxypropane (TEP), cytochrome C, xanthine oxidase, xanthine, glutathione reductase, nicotinamide adenine dinucleotide phosphate (NADPH), hydrogen peroxide, superoxide dismutase, glutathione peroxidase, catalase, acetylthiocholine iodide (ATCI), acetylcholinesterase, 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), cresyl violet, sodium acetate, sodium carbonate, 2,4,6-tripyridyl-striazine (TPTZ), Folin-Ciocalteu reagent, gallic acid, ascorbic acid, Trizma hydrochloride, potassium chloride, 2,2-diphenyl-1-picrylhydrazyl (DPPH), tris-hydrochloride, and sodium carbonate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Chemicals used in Western blot analysis were purchased from Bio-Rad Laboratories. Methanol and acetic acid (HPLC grade) were purchased from Fisher Scientific.
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