A 0061

Manufactured by Agilent Technologies

Sourced in Switzerland

The A-0061 is a lab equipment product offered by Agilent Technologies. It serves as a core instrument for various laboratory applications. The detailed technical specifications and intended use of this product are not available within the scope of this request.

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Lab products found in correlation

Peroxidase substrate kit, by Vector Laboratories (1 mentions) Potassium ferrocyanide, by Merck Group (1 mentions) F5012, by Merck Group (1 mentions) Vectastain kit, by Vector Laboratories (1 mentions) Ab84903, by Abcam (1 mentions) Ab2219, by Merck Group (1 mentions) Ab40873, by Abcam (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions) Rabbit anti egfr, by Santa Cruz Biotechnology (1 mentions) A0021, by Agilent Technologies (1 mentions) Mouse anti flotillin 1, by BD (1 mentions) Wga fitc conjugate, by Merck Group (1 mentions) Rabbit anti rfp, by Rockland Immunochemicals (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) Rabbit anti gapdh, by Cell Signaling Technology (1 mentions) Mouse anti eea1, by BD (1 mentions)

2 protocols using a 0061

Lung Iron Detection using Perls DAB Staining

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Ferric iron was detected on serial 5-µm paraformaldehyde-fixed, paraffin-embedded lung sections using Perls DAB staining. Briefly, slides were incubated for 30 min in 5% potassium ferrocyanide (Sigma-Aldrich, St. Louis, MO) and 5% hydrochloric acid, followed by a 15 min incubation in DAB (Peroxidase Substrate Kit; Vector Laboratories, Burlingame, CA). Negative control slides were incubated for 30 min in PBS and 15 min in DAB. Immunohistochemical staining for macrophages, transferrin or ferritin was performed using a Vectastain kit (Vector Laboratories; Burlingame, CA) using mouse anti-CD68 (1∶100, KP1; Dako, Kyoto, Japan), rabbit anti-transferrin (1∶30 000, A-0061; DakoCytomation, Carpinteria, CA), or anti-H-ferritin (1∶2000, F-5012; Sigma) antibodies, respectively. Isotype-matched, nonimmune immunoglobulins served as negative controls.

Philippot Q., Deslée G., Adair-Kirk T.L., Woods J.C., Byers D., Conradi S., Dury S., Perotin J.M., Lebargy F., Cassan C., Le Naour R., Holtzman M.J, & Pierce R.A. (2014). Increased Iron Sequestration in Alveolar Macrophages in Chronic Obtructive Pulmonary Disease. PLoS ONE, 9(5), e96285.

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Antibody Immunodetection Protocol

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The following antibodies were used: rabbit anti-human transferrin (A0061, Dako); rabbit anti-human Gc-globulin (also known as VDBP, A0021, Dako); rabbit anti-uteroglobin (also known as CC16, ab40873, Abcam); rabbit anti-SLC1A5 (also known as SGLT2, ab84903, Abcam); rabbit NaPi-IIa (gift from C.A.Wagner, University of Zurich, Zurich, Switzerland); rabbit anti-human AQP1 (ab2219, Millipore); mouse anti-β-actin (A2228, Sigma-Aldrich); mouse conjugated to Fluorescein (FITC) anti-PI(4,5)P₂ (Z-G045, Echelon Biosciences Inc.); mouse anti-EEA1 (610456, BD Bioscience); rabbit anti-RFP (600–401-379, ROCKLAND); sheep anti-LRP2 (gift from P. Verroust and R. Kozyraki, INSERM, Paris, France); mouse anti Flotillin-1(610821, BD Bioscience); mouse anti-α-tubulin (T5168, Sigma-Aldrich); rabbit anti-GAPDH (2118, Cell Signaling Technology); rat anti-LAMP1 (sc-19992, Santa Cruz Biotechnology); goat anti-Cathepsin-D (Cts-D; sc-6486, Santa Cruz Biotechnology); rabbit anti-EGFR (1005 sc-03, Santa Cruz Biotechnology); Alexa-488 Phalloidin (F-actin, A12379, Thermofisher Scientific); mouse anti-Transferrin Receptor Antibody (H68,4, ThermoFisher Scientific); WGA FITC Conjugate (L 4895, Sigma-Aldrich); mouse anti-Na⁺/K⁺-ATPase subunit α1 (C464.6 EMD Millipore); rabbit anti-MPR and rabbit anti-OCRL (gift from A. De Matteis, Telethon Institute of Genetics and Medicine (TIGEM), Pozzuoli, Italy).

Festa B.P., Berquez M., Gassama A., Amrein I., Ismail H.M., Samardzija M., Staiano L., Luciani A., Grimm C., Nussbaum R.L., De Matteis M.A., Dorchies O.M., Scapozza L., Wolfer D.P, & Devuyst O. (2018). OCRL deficiency impairs endolysosomal function in a humanized mouse model for Lowe syndrome and Dent disease. Human Molecular Genetics, 28(12), 1931-1946.

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