4 hydroxy 3 methoxycinnamic acid

Manufactured by Merck Group

Sourced in Germany, Spain

4'-hydroxy-3'-methoxycinnamic acid is a chemical compound used in laboratory settings. It is a phenolic acid that functions as an antioxidant. The compound can be utilized in various analytical and experimental procedures within the scientific community.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

4-methoxycinnamic acid (2 citations)

6 protocols using 4 hydroxy 3 methoxycinnamic acid

Formulation of a Nutraceutical Blend

Check if the same lab product or an alternative is used in the 5 most similar protocols

B-Can™ 70% Oat Beta-Glucan purchased in Garuda International, Inc. (California, United States), and a decaffeinated phenol-rich (45%) green coffee extract acquired from Quimifarma Laboratorios S.L. (Toledo, Spain), were selected for the design of the nutraceutical. The mixture of 3.57 g BG and 0.66 g of GCPE (considering their purity) was flavoured (vanilla and chocolate) to increase its acceptability and was presented in single-dose sachets for dispersion in water (Quimifarma Laboratorios S.L. Toledo, Spain).
All solvents and reagents were of analytical grade unless otherwise stated. Ascorbic; 5caffeoylquinic acid; 3´,4´-dihydroxycinnamic acid; 4´-hydroxy-3´-methoxycinnamic acid; 4´hydroxycinnamic acid; hippuric acid; 3-(3´,4´-dihydroxyphenyl)propanoic acid; 3-(4´-hydroxy-3´methoxyphenyl)propanoic acid;
3-(4´-hydroxyphenyl)propanoic acid;
3-(3´hydroxyphenyl)propanonic acid; 3´,4´-dihydroxyphenylacetic acid; 4´-hydroxy-3´methoxyphenylacetic acid; 4´-hydroxyphenylacetic acid; 3´-hydroxyphenylacetic acid; 3,4dihydroxybenzoic acid; 4-hydroxy-3-methoxybenzoic acid; 4-hydroxybenzoic acid and 3hydroxybenzoic acid were from Sigma-Aldrich (Madrid, Spain). 3,5-dicaffeoylquinic acid was purchased from PhytoLab (Vestenbergsgreuth, Germany). Methanol, formic acid and acetonitrile (HPLC grade) were acquired from Panreac (Madrid, Spain).

Seguido M.Á., Tarradas R.M., González-Rámila S., García-Cordero J., Sarriá B., Bravo-Clemente L, & Mateos R. (2022). Influence of 8-week daily consumption of a new product combining green coffee hydroxycinnamates and beta-glucans on polyphenol bioavailability in subjects with overweight and obesity. Food & function, 13(3).

+ Open protocol

+ Expand

Antioxidant Capacity Determination Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reference compounds 3,4-dihydroxycinnamic acid, chlorogenic acid, 4-hydroxy-3-methoxycinnamic acid, 4′-hydroxycinnamic acid, 3,4,5-trihydroxycinnamic acid, 4-hydroxybenzoic acid, (−-epicatechin, apigenin, cyanidin-3-glucoside, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), and β-carotene, and the individual sugars glucose, fructose, and sucrose, were obtained from Sigma-Aldrich (Steinheim, Germany). Formic acid was obtained from Sigma-Aldrich. LC-MS grade water, m ethanol, ethanol, dichloromethane, and acetonitrile were acquired from PanReac Applichem (Castellar del Vallés, Barcelona, Spain).
Note: the names for the phenolic compounds used in this paper are based on the nomenclature thesaurus of Kay et al., 2020 [16 (link)].
The reagents 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH), fluorescein, potassium persulfate, sodium hydroxyde, potassium dihydrogen phosphate, and sodium hydrogen phosphate were also acquired from Sigma-Aldrich (Steinheim, Germany).

Ordóñez-Díaz J.L., Velasco-Ruiz I., Velasco-Tejero C., Pereira-Caro G, & Moreno-Rojas J.M. (2024). Seasonal and Morphology Effects on Bioactive Compounds, Antioxidant Capacity, and Sugars Profile of Black Carrot (Daucus carota ssp. sativus var. atrorubens Alef.). Foods, 13(10), 1575.

+ Open protocol

+ Expand

Quantification of Polyphenol Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ascorbic, 5-caffeoylquinic acid, 3′,4′-dihydroxycinnamic acid, 4′-hydroxy-3′-methoxycinnamic acid, 4′-hydroxycinnamic acid; hippuric acid, 3-(3′,4′-dihydroxyphenyl)propanoic acid, 3-(4′-hydroxy-3′-methoxyphenyl)propanoic acid, 3-(4′-hydroxyphenyl)propanoic acid, 3-(3′-hydroxyphenyl)propanoic acid, 3′,4′-dihydroxyphenylacetic acid, 4′-hydroxy-3′-methoxyphenylacetic acid, 4′-hydroxyphenylacetic acid, 3′-hydroxyphenylacetic acid, 3,4-dihydroxybenzoic acid, 4-hydroxy-3-methoxybenzoic acid, 4-hydroxybenzoic acid, and 3-hydroxybenzoic acid were purchased from Sigma-Aldrich (Madrid, Spain). 3,5-dicaffeoylquinic acid was purchased from PhytoLab (Vestenbergsgreuth, Germany). HPLC-grade solvents were acquired from Panreac (Madrid, Spain). Ultrapure water from MilliQ system (Millipore, Bedford, MA, USA) was used throughout the experiment.

Seguido M.Á., Tarradas R.M., González-Rámila S., García-Cordero J., Sarriá B., Bravo-Clemente L, & Mateos R. (2022). Sustained Consumption of a Decaffeinated Green Coffee Nutraceutical Has Limited Effects on Phenolic Metabolism and Bioavailability in Overweight/Obese Subjects. Nutrients, 14(12), 2445.

+ Open protocol

+ Expand

Standardized LC-MS/MS Phenolic Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Methanol for
LC–MS analysis was purchased from Panreac AppliChem (Darmstadt,
Germany). Acetonitrile and 99% formic acid (both LC–MS grades)
were obtained from Scharlau (Barcelona, Spain). The pure phenolic
standards for LC–MS/MS were acquired from different manufacturers.
In order to standardize, the recommended nomenclature of (poly)phenols
proposed by Kay et al.^{30 (link)} is used in the
present study (Table S1). Apigenin-7-O-glucoside, apigenin-8-C-glucoside, apigenin-6,8-C-diglucoside, luteolin, luteolin-7-O-glucoside,
luteolin-8-C-glucoside, quercetin, quercetin-3-O-rutinoside, quercetin-3-O-glucoside,
quercetin-3-O-rhamnoside, isorhamnetin, 4-caffeoylquinic
acid, 5-caffeoylquinic acid, 4′-hydroxycinnamic acid, 3′,4′-dihydroxycinnamic
acid, 4′-hydroxy-3′-methoxycinnamic acid, 3′-hydroxy-4′-methoxycinnamic
acid, 4-hydroxy-3′,5′-dimethoxycinnamic, benzene-1,2-diol,
3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 2,5-dihydroxybenzoic
acid, 3,4-dihydroxybenzoic acid, and 4-hydroxy-3-methoxybenzoic acid
were purchased from Sigma-Aldrich (Darmstadt, Germany). Standards
of 3-(4′-hydroxy-3′-methoxyphenyl)propanoic acid and
3-(3′,4′-dihydroxyphenyl)propanoic acid were obtained
from Alfa Aesar (Kandel, Germany). Naringenin-7-O-glucoside, kaempferol-7-O-glucoside, isorhamnetin-3-O-glucoside, and 2-(3′-hydroxyphenyl)ethanol, were
acquired from Extrasynthese (Lyon, France).

Del Burgo-Gutiérrez C., Cid C., Ludwig I.A, & De Peña M.P. (2023). LC–MS/MS Analysis Elucidates the Different Effects of Industrial and Culinary Processing on Total and Individual (Poly)phenolic Compounds of Piquillo Pepper (Capsicum annuum cv. Piquillo). Journal of Agricultural and Food Chemistry, 71(15), 6050-6060.

+ Open protocol

+ Expand

Antioxidant Assays Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

1,1-Diphenyl-2-picrylhydrazyl (DPPH), butylated hydroxytoluene (BHT), nitro blue tetrazolium dihydrochloride (NBT), phenazine methosulphate (PMS), nicotinamide adenine dinucleotide (NADH), 2-deoxy-D-ribose, hydrogen peroxide, disodium ethylenediaminetetracetate (EDTA), trichloroacetic acid (TCA), ferric chloride, thiobarbituric acid (TBA), and ascorbic acid (AA) were purchased from Sigma (Sigma-Aldrich GmbH, Steinheim is the correct city nameSteinheim, Germany). All other chemicals were of analytical grade.
Phenolic standards: ellagic acid (95%), syringic acid (98%), epi catechin (96%), 4-hydroxy-3-methoxy-cinnamic acid (95%), rutin (95%), vanillic acid (95%), 3-hydroxybenzoic acid (95%), protocatechuic acid (96%), caffeic acid (95%), coumaric acid (98%), epigallo catechin (97%), catechin (95%), quercetin (95%), and resveratrol (99%) were purchased from Sigma (Sigma-Aldrich GmbH, Steinheim is the correct city nameSteinhiem, Germany). Ferulic acid (97%) and chlorogenic acid (95%) were purchased from the European Pharmacopoeia (EP).

Untea A.E., Oancea A.G., Vlaicu P.A., Varzaru I, & Saracila M. (2024). Blackcurrant (Fruits, Pomace, and Leaves) Phenolic Characterization before and after In Vitro Digestion, Free Radical Scavenger Capacity, and Antioxidant Effects on Iron-Mediated Lipid Peroxidation. Foods, 13(10), 1514.

+ Open protocol

+ Expand

Phenolic Compounds Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Carbon dioxide (mass fraction
purity 0.999 in the liquid phase) was supplied by Sapio S.r.l. (Udine,
Italy). Sep-Pak Plus tC18 cartridge WAT 036810 and WAT036800 were
purchased from Waters (Milan, Italy). The Folin–Ciocalteau
reagent, 2,2-difenil-1-picrylhydrazyl, reagents of analytical grade
or higher available purity, (−)-epicatechin, (+)-catechin,
gallic acid, 3,4-dihydroxybenzoic acid (protocatechuic acid), 4-hydroxy-3,5-dimethoxybenzoic
acid (syringic acid), 3,4-dihydroxycinnamic acid (caffeic acid), 4-hydroxy-3-methoxycinnamic
acid (ferulic acid), 4-hydroxycinnamicacid (p-coumaric
acid), and trans-resveratrol were purchased from
Sigma-Aldrich (Milan, Italy).
The other phenolic compounds were
quercetin, isoquercitrin (quercetin-3-O-glucoside),
kaempferol, kaempferol-3-O-glucoside, rhamnetin,
isorhamnetin, isorhamnetin-3-O-glucoside, rutin (quercetin-3-O-rutinoside), and myricetin and were supplied by Extrasynthese
(Lyon, France).

Da Porto C., Natolino A, & Scalet M. (2022). Improved Sustainability in Wine Industry Byproducts: A Scale-up and Economical Feasibility Study for High-Value Compounds Extraction Using Modified SC-CO2. ACS Omega, 7(38), 33845-33857.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!