During the animal’s light cycle between 10:00 and 13:00, single-housed mice were restrained for 15 min in modified 50 ml conical tubes with the cone endings removed and an aperture added to the cap for the tail. Tail blood was collected with heparin-coated capillary tubes (Drummond) at time points 0, 15, 30 and 90 min from the onset of restraint stress. Plasma was collected from blood by centrifugation at 2,000× g for 10 min. CORT levels were measured in plasma using CORT ELISA kit (Enzo Life Sciences). Using 5 μL of plasma, samples were prepared and analyzed in duplicate on a 96-well plate following manufacturer’s protocol.
Heparin coated capillary tubes
Manufactured by Drummond
Sourced in United States
Heparin-coated capillary tubes are specialized laboratory equipment used for the collection and analysis of blood samples. The capillary tubes are coated with the anticoagulant heparin, which prevents the blood from clotting during the collection and testing processes.
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Lab products found in correlation
4 protocols using heparin coated capillary tubes
1
Restraint Stress Induces Corticosterone
2
Ethanol Consumption in Mice Drinking in the Dark
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The drinking in the dark assay was performed based on published methods (Thiele et al., 2014 (link)). For four nights, single-housed mice were habituated in the home cage to one 25 ml sipper tube filled with water that replaced their regular water bottle 2 h into the animal’s dark cycle. After the final habituation trial, sipper tubes were filled with 20% (w/vol) ethanol and consumption was measured for 2 h training trials over three consecutive days and for 4 h in a test trial on the final day.
To examine blood ethanol concentrations (BEC) immediately following the test trial, ≤10 μL tail vein blood was collected from each animal using heparin-coated capillary tubes (Cat# 1-000-3200-H; Drummond, Broomall, PA, USA). Tail blood was centrifuged at 2,000× g for 10 min and plasma was stored at −80°C prior to being measured for ethanol (mg/dL) using an AM1 Analox Ethanol analyzer (Analox Instruments, London, UK).
To examine blood ethanol concentrations (BEC) immediately following the test trial, ≤10 μL tail vein blood was collected from each animal using heparin-coated capillary tubes (Cat# 1-000-3200-H; Drummond, Broomall, PA, USA). Tail blood was centrifuged at 2,000× g for 10 min and plasma was stored at −80°C prior to being measured for ethanol (mg/dL) using an AM1 Analox Ethanol analyzer (Analox Instruments, London, UK).
3
Restraint Stress Response in Mice
4
Stress-Induced Corticosterone Response
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