Cfpac 1

PC cell lines SW1990 and CFPAC-1 and normal pancreatic duct epithelial cell line HPDE were acquired from BeNa Culture Collection (Beijing, China). All cells were cultured in Roswell Park Memorial Institute-1640 (RPMI-1640) medium (Gibco, Carlsbad, CA, USA) added with 10% fetal bovine serum (FBS; Gibco), penicillin (100 units/mL) and streptomycin (100 μg/mL) at 37°C atmosphere with 5% CO₂.

Human pancreatic ductal cell line (HPNE) and PC cell lines, including PANC-1, SW1990,
HS766T, and CFPAC-1, were purchased from BeNa Culture Collection (Beijing, China) and
cultured in incubator with 100% humidity and 5% CO₂ at 37°C. The HPNE cells
were grown in Dulbecco modified Eagle medium (DMEM; Sigma, St Louis, Missouri) containing
1 volume of M3 Base F culture medium (InCell Corp, San Antonio, Texas), 3 volumes of
glucose-free DMEM, 10% fetal bovine serum (FBS; (Invitrogen, Carlsbad, California), 5.5 mM
glucose, 10 ng/mL epidermal growth factor (EGF), and 50 µg/mL gentamycin. The DMEM medium
with 10% FBS was applied to culture PANC-1 and HS766T cells. SW1990 cells were maintained
in Leibovitz L-15 Medium (Sigma). Furthermore, CFPAC-1 cells were cultured in Iscove
Modified Dulbecco Medium (Sigma) adding 10% FBS.

The cells PANC-1, CFPAC-1, ASPC-1, BXPC-3, CAPAN-1, CAPAN-2 and normal human pancreatic duct epithelial (HPDE6-C7) were purchased from BeNa Culture Collection (Bejing, China). Human embryonic kidney 293T cells were purchased from the Cell Repository of the Chinese Academy of Sciences (Shanghai, China). All cells were cultured in DMEM or RPMI1640 or IMDM supplemented with 10% serum, 100 U/ml penicillin (Beyotime, China), and 100 mg/mL streptomycin (Beyotime, China) at 37 °C with 5% CO2.