Eight-color flow cytometry was conducted using total mononuclear cell preparations and was run on the MACSQuant Analyzer (Miltenyi, Auburn, CA). Antibodies purchased from eBioscience are as follows: anti-CD11b (M1/70); anti-CD18 (M18/2); anti-Ly-6G (RB6-8C5); anti-CD22 (2D6); anti-CD69 (H1.2F3); anti-MHC II (M5/114.15.2); anti-TCRβ (H57-597); anti-CD44 (IM7); anti-CD14 (Sa2-8); anti-CD45 (30-F11); anti-CD62L (MEL-14); anti-CD209 (5H10); anti-CD20 (2H7); anti-IFNγ (XMG1.2); anti-TNF-α (MP6-XT22); anti-FoxP3 (FJK-16s); and anti-IL-6 (MP5-20F3). Anti-CD8a-VioBlue was purchased from Miltenyi Biotech. Antibodies made in-house include: anti-CD4 (GK1.5); anti-CD40 (1C10); anti-CD25 (PC61.5.3); anti-CD5 (53-7.313); and anti-CD3 (145-2C11). Single cell suspensions were incubated on ice with appropriate antibodies for 30 min. Cells were washed with running buffer 3 times fixed in 1% paraformaldehyde/PBS, and then suspended in running buffer for flow cytometry. Results were analyzed with FlowJo software.
Anti ly 6g rb6 8c5
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-Ly-6G (RB6-8C5) is a monoclonal antibody that recognizes the Ly-6G antigen, which is specifically expressed on the surface of mouse neutrophils. This antibody is commonly used in flow cytometry and other immunological applications to identify and study neutrophil populations.
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Lab products found in correlation
Anti cd45, by Thermo Fisher Scientific (1 mentions)
Anti tcrβ, by Thermo Fisher Scientific (1 mentions)
Anti tnf α mp6 xt22, by Thermo Fisher Scientific (1 mentions)
Anti foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions)
Anti mhcii, by Thermo Fisher Scientific (1 mentions)
Anti cd44, by Thermo Fisher Scientific (1 mentions)
Anti cd69, by Thermo Fisher Scientific (1 mentions)
Anti cd20, by Thermo Fisher Scientific (1 mentions)
Macsquant analyzer, by Miltenyi Biotec (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
2 protocols using anti ly 6g rb6 8c5
1
Multiparameter Flow Cytometry of Immune Cell Subsets
2
Immunohistochemical Staining of Mouse Uterine Tissue
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Frozen section (8 μm) of mouse uterine tissue was fixed in pre-cold cooled methanol for 10 min., Then, after the tissue was incubated with 0.3% H2O2 for 10 min at room temperature, and the slides were blocked in 5% bovine serum albumin (BSA) (Invitrogen, CA) for 1 h at 37 °C. Next, the sections were incubated with anti-Ly-6G (RB6-8C5; eBiosciences, San Diego, CA, USA) diluted in PBS (1:500) overnight at 4 °C. Then, the sections were incubated with a secondary antibody conjugated to HRP at 37 °C for 1 h. Finally, the slides were stained with diaminobenzidine and counterstained with haematoxylin, and then observed and photographed using a Nikon H600L (Japan) microscope.
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