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Goat anti choline acetyltransferase

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Goat anti-choline acetyltransferase is a laboratory reagent used in biochemical and neurological research. It is an antibody produced in goats that specifically binds to the enzyme choline acetyltransferase, which is involved in the synthesis of the neurotransmitter acetylcholine.

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Lab products found in correlation

Goat anti vacht, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Alexa 488, by Thermo Fisher Scientific (2 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Goat anti sox2, by Santa Cruz Biotechnology (1 mentions) Rabbit anti ha, by Cell Signaling Technology (1 mentions) Fluoromount g, by Southern Biotech (1 mentions) Mouse anti ki67, by BD (1 mentions) Rabbit anti calbindin, by Swant (1 mentions) Prolong gold antifade, by Thermo Fisher Scientific (1 mentions) Mouse anti calretinin, by Merck Group (1 mentions) Anti rbpms, by Abcam (1 mentions) Mouse anti gad65 67, by Merck Group (1 mentions) Goat anti anti chx10, by Santa Cruz Biotechnology (1 mentions) Rabbit anti secretagogin, by BioVendor (1 mentions) Mouse anti satb2, by Abcam (1 mentions) Anti gfp, by Abcam (1 mentions) Rabbit anti neun, by Merck Group (1 mentions) Mouse anti glial fibrillary acidic protein gfap, by Merck Group (1 mentions) Leica cm3050, by Leica Microsystems (1 mentions)

5 protocols using goat anti choline acetyltransferase

1

Immunostaining of Retinal Tissues

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Retinal tissues were prepared as described above. Antibodies used were as follows: chick and rabbit anti-GFP (1:500, Abcam; 1:5000, Millipore; 1:1000, AVES); goat anti-choline acetyltransferase (1:500, Millipore); goat anti-VAChT (1:1000, Millipore); goat anti-Sox2 (1:100, Santa Cruz); mouse anti-Isl1 (1:10, DSHB), rabbit anti-HA (1:500, Cell Signaling), and rabbit anti-DsRed (1:1000, Living Colors), and mouse anti-Ki67 (1:200, BD Biosciences). Nuclei were labeled with DAPI (1:1000, Invitrogen). Secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (Invitrogen) and used at 1:1000. Fluoromount-G (SouthernBiotech) was used for mounting wholemounts. ProLong Gold Antifade was used for mounting retina section slides.
Peng Y.R., James R.E., Yan W., Kay J.N., Kolodkin A.L, & Sanes J.R. (2019). Binary Fate Choice between Closely Related Interneuronal Types Is Determined by A Fezf1-Dependent Postmitotic Transcriptional Switch. Neuron, 105(3), 464-474.e6.
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2

Immunostaining for Retinal Cell Markers

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Tissue was fixed as described above. Immunostaining was performed as described in refs. 12 and 31. Antibodies used were as follows: chick and rabbit anti-GFP (1:500, Abcam; 1:2000, Millipore); mouse anti-Satb2(1:1000, Abcam); goat anti anti-CHX10 (1:300, Santa Cruz); rabbit anti-TFAP2B (1:1000, cell signaling); goat anti-Choline Acetyltransferase (1:500, Millipore); goat anti-VAChT (1:1000, Millipore); rabbit and guinea pig anti-RBPMS (1:1000, Abcam; 1:5000, PhosphoSolutions); rabbit anti-Calbindin (1:2000, Swant); mouse anti-Calretinin (1:5000, Millipore); mouse anti-Gad65/67 (1:1000, Millipore); mouse and rabbit anti-PKCa (1:2000, Abcam; 1:2000, sigma); rabbit anti-Secretagogin (1:10,000; BioVendor); mouse anti-Human CD15 (1:100, BD); rabbit anti-GNGT1 (1:1000, gift from Dr. N. Gautam), mouse anti-7G6 (1:1000, gift from Dr. Peter MacLeish), and chick anti-OPN1M/LW (1:2000, gift from Dr. Jeremy Nathans). Nuclei were labeled with DAPI (1:1000, Invitrogen). Secondary antibodies were conjugated to Alexa Fluor 488, 568, and 647 (Invitrogen) and used at 1:1000. Sections and whole mounts were mounted in ProLong Gold Antifade (Invitrogen).
Peng Y.R., Shekhar K., Yan W., Herrmann D., Sappington A., Bryman G.S., van Zyl T., Do M.T., Regev A, & Sanes J.R. (2019). MOLECULAR CLASSIFICATION AND COMPARATIVE TAXONOMICS OF FOVEAL AND PERIPHERAL CELLS IN PRIMATE RETINA. Cell, 176(5), 1222-1237.e22.
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3

SCI Tissue Preparation and Immunohistochemistry

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Six weeks after SCI, the rats were deeply anesthetized using sodium pentobarbital, perfused with PBS, and fixed using a 4% PFA solution delivered transcardially. The cervical region and the lumbar region (C1–C3 of the cervical enlargement and L4–L6 of the lumbar enlargement, respectively), and the epicenter of the SCI were removed and post-fixed in a 4% PFA solution for an additional 24 hours at 4°C. The tissues were cut transversely into 20-μm-thick sections on a cryostat (Leica CM3050S, Leica Microsystems). The tissue sections were incubated with diluted primary antibodies (goat-anti choline acetyltransferase, 1:100, Millipore; rabbit-anti serotonin 2A receptor, 1:200, Calbiochem; rabbit-anti NeuN, 1:500, Millipore; goat-anti serotonin, 1:500, Abcam; mouse-anti glial fibrillary acidic protein [GFAP], 1:500, Millipore) overnight. The next day, the sections were incubated with fluorescent secondary antibodies (Alexa Fluor 488 and 568 for each species-of primary antibody, 1:200, Life Technologies) and DAPI (1:1,000, Sigma) for 2 hours. All images were acquired using a BZ-9000 HS All-in-one Fluorescence Microscope (Keyence BZ-9000).
Ryu Y., Ogata T., Nagao M., Kitamura T., Morioka K., Ichihara Y., Doi T., Sawada Y., Akai M., Nishimura R, & Fujita N. (2017). The swimming test is effective for evaluating spasticity after contusive spinal cord injury. PLoS ONE, 12(2), e0171937.
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4

Comprehensive Immunolabeling for Neuroscience

Cited 1 time
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Primary antibodies used included the following: Guinea pig anti-VGLUT3 (1:10,000, Millipore), sheep anti-tyrosine hydroxylase (1:500, Millipore), rabbit anti-melanopsin (1:10,000, ATS), mouse anti-Brn3a (1:500, Millipore), goat anti-choline acetyltransferase (1:500, Millipore), mouse anti-NeuN (1:500, Millipore), mouse anti-GFAP (1:500, Sigma), rabbit anti-FoxP2 (1:4000, Abcam), rabbit anti-Pax2 (1:200, Zymed), rabbit anti-cleaved caspase 3 (1:100, Cell Signaling Technologies), mouse anti-parvalbumin (1:500, Sigma), and mouse anti-GAPDH (1:500, Abcam). Mouse monoclonal antibodies against γ-Pcdh proteins used for western blots (1:500–1:1000) were generated by NeuroMab in collaboration with the Weiner laboratory [55 (link)] and obtained from Antibodies, Inc.: N159/5 (detecting an epitope in constant exon 1 or 2 and thus all 22 γ-Pcdh isoforms); N144/32 (detecting all γA subfamily isoforms); N148/30 (specific for γB2); N174B/27 (specific for γC3). A rabbit polyclonal antibody raised at Affinity BioReagents against the peptide sequence VAGEVNQRHFRVDLD (within EC1) from murine γC4 was also used for western blotting (1:1000). Secondary antibodies were conjugated with Alexa-488, -568, or -647 (1:500, Invitrogen) or HRP (1:1000–1:5000, Jackson Immunoresearch).
Garrett A.M., Bosch P.J., Steffen D.M., Fuller L.C., Marcucci C.G., Koch A.A., Bais P., Weiner J.A, & Burgess R.W. (2019). CRISPR/Cas9 interrogation of the mouse Pcdhg gene cluster reveals a crucial isoform-specific role for Pcdhgc4. PLoS Genetics, 15(12), e1008554.
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5

Immunoblotting and Immunofluorescence Antibodies

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The following antibodies were used in immunoblotting and immunofluorescence experiments: rabbit anti-human p75NTR (1:1,000; G3231; Promega), rabbit anti-TrkA (1:1,000; Millipore), rabbit anti-phosphoTyr674/5 (1:1,000; Cell Signaling), mouse anti-HA (1:2,000; Sigma-Aldrich), mouse anti-FLAG M2 (1:1,000; Sigma-Aldrich), mouse anti β-actin (1:1,000; Sigma-Aldrich), rabbit MBP-probe (1:1,000; Santa Cruz), rabbit anti-Cleaved Caspase-3 (1:1,000, 9661S; Cell Signaling), rabbit anti phospho-p38 (1:1,000, 9211; Cell Signaling), rabbit anti p38 (1:1,000, 9212; Cell Signaling), rabbit anti JNK (1:1,000, 9252; Cell Signaling), rabbit anti phospho-JNK (1:1,000, 9251; Cell Signaling), goat anti-choline acetyltransferase (1:200, AB144P; Millipore), rabbit anti Cy3 (1:500; Jackson), goat anti mouse Ig/HRP (1:10,000; Jackson), goat anti rabbit Ig/HRP (1:10,000; Jackson), goat IRDye800 (1:15,000; Rockland), and goat anti-mouse antibodies coupled to either Alexa 555 or Alexa 488 (Invitrogen). The DNA was stained with DAPI (1:1,000).
Franco M.L., García-Carpio I., Comaposada-Baró R., Escribano-Saiz J.J., Chávez-Gutiérrez L, & Vilar M. (2021). TrkA-mediated endocytosis of p75-CTF prevents cholinergic neuron death upon γ-secretase inhibition. Life Science Alliance, 4(4), e202000844.
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