L valine

Manufactured by Fujifilm

Sourced in Japan

L-valine is a type of amino acid commonly used in laboratory and research settings. It serves as a building block for proteins and is involved in various metabolic processes. L-valine can be used in the production of cell culture media and as a component in various analytical and diagnostic applications.

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Lab products found in correlation

L alanine, by Fujifilm (6 mentions) L methionine, by Fujifilm (6 mentions) L isoleucine, by Fujifilm (5 mentions) L leucine, by Fujifilm (5 mentions) L phenylalanine, by Fujifilm (5 mentions) L serine, by Fujifilm (4 mentions) L threonine, by Fujifilm (4 mentions) L glycine, by Fujifilm (3 mentions) L proline, by Fujifilm (3 mentions) L tryptophan, by Fujifilm (3 mentions) L tyrosine, by Fujifilm (3 mentions) L glutamine, by Fujifilm (3 mentions) L histidine, by Fujifilm (3 mentions) Ethanol, by Fujifilm (3 mentions) L asparagine monohydrate, by Fujifilm (2 mentions) L aspartic acid, by Fujifilm (2 mentions) L glutamic acid, by Fujifilm (2 mentions) L arginine, by Fujifilm (2 mentions) L asparagine, by Fujifilm (2 mentions) L lysine, by Fujifilm (2 mentions)

Spelling variants of this product

Valine (3 citations)

9 protocols using l valine

Biochemical Compounds Procurement Protocol

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NAD disodium salt, d-(–)-3-phosphoglyceric acid disodium salt, and l-homocysteine were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). l-Alanine, l-valine, l-methionine, l-homoserine, l-serine, and N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), DTT, NADH, and isopropyl-β-d-thiogalactopyranoside (IPTG) were purchased from FUJIFILM Wako Pure Chemical Industries, Ltd. (Osaka, Japan).

Okamura E., Ohtaka K., Nishihama R., Uchida K., Kuwahara A., Mochida K, & Hirai M.Y. (2021). Diversified amino acid-mediated allosteric regulation of phosphoglycerate dehydrogenase for serine biosynthesis in land plants. Biochemical Journal, 478(12), 2217-2232.

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Taste Receptor Activation by Amino Acids

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Agar (purified powder) was obtained from Sigma-Aldrich (St Louis, MO, USA). As tastants, D-fructose (CAS: 57-48-7, Wako Pure Chemical Industries, Ltd., Osaka, Japan), as well as L-alanine (ALA; CAS: 56-41-7), L-arginine monohydrochloride (ARG; CAS: 1119-34-2), L-asparagine monohydrate (ASN; CAS: 5794-13-8), L-aspartic acid (ASP; CAS: 56-84-8), L-cysteine monohydrochloride hydrate (CYS; CAS: 7048-04-6), L-histidine monohydrochloride (HIS; CAS: 645-35-2), L-isoleucine (ILE; CAS: 73-32-5), L-leucine (LEU; CAS: 61-90-5), L-lysine monohydrochloride (LYS; CAS: 657-27-2), L-glutamine (GLN; CAS: 56-85-9), L-glutamic acid (GLU; CAS: 56-86-0), L-glycine (GLY; CAS: 56-40-6), L-methionine (MET; CAS: 63-68-3), L-phenylalanine (PHE; CAS: 63-91-2), L-proline (PRO; CAS: 147-85-3), L-serine (SER; CAS: 56-45-1), L-threonine (THR; CAS: 72-19-5), L-tryptophan (TRP; CAS: 73-22-3), L-tyrosine (TYR; CAS: 60-18-4) and L-valine (VAL; CAS: 72-18-4) were used at concentrations of 10 mmol l⁻¹ (TYR was used in 0.5 mmol l⁻¹ due to low solubility). Amino acids were obtained from Wako Pure Chemical Industries, Ltd., Osaka, Japan (TYR, SER, CYS, VAL), Sigma-Aldrich, St Louis, MO, USA (ILE, PRO) or Nacalai Tesque, Kyoto, Japan (ALA, ARG, ASN, ASP, LEU, LYS, GLN, GLY, MET, PHE, THR, TRP, TYR).

Kudow N., Miura D., Schleyer M., Toshima N., Gerber B, & Tanimura T. (2017). Preference for and learning of amino acids in larval Drosophila. Biology Open, 6(3), 365-369.

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Synthesis and Analysis of Amino Acids

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L-Kynurenine sulfate salt, KYNA, and L-tyrosine disodium salt hydrate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). L-Alanine, L-arginine, L-asparagine, L-aspartate, L-cysteine hydrochloride monohydrate, L-glutamine, L-glutamate, L-glycine, L-histidine, L-isoleucine, L-leucine hydrochloride, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, and L-valine were purchased from Wako Pure Chemical Industries (Osaka, Japan). Rodent diet (MF) was obtained from Oriental Yeast Co., Ltd (Tokyo, Japan). All other chemicals were of the highest commercially available purity.

Sekine A., Okamoto M., Kanatani Y., Sano M., Shibata K, & Fukuwatari T. (2015). Amino acids inhibit kynurenic acid formation via suppression of kynurenine uptake or kynurenic acid synthesis in rat brain in vitro. SpringerPlus, 4, 48.

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Electrochemical Biosensor for Lactic Acid

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L-lactic acid (CAS: 79-3344, 129-02666), sodium chloride (CAS: 7647-14-5, 195-15975), potassium dihydrogen phosphate (CAS: 7778-77-0,164-22635), disodium hydrogen phosphate (CAS: 7558-79-4, 042-30055), urea (CAS: 57-13-6, 215-00172), ethanol (CAS: 64-17-5, 057-00456), D-glucose (CAS: 50-99-7, 049-31165), L-valine (CAS: 72-18-4, 228-00082), L-leucine (CAS: 61-90-5, 124-00852), and ammonia (CAS: 1336-21-6, 013-17505) were all purchased from Wako, Japan. Lactate oxidase (LOD) from Aerococcus viridans (T-47, Asahi Kasei Pharma, Tokyo, Japan) was purchased from Asahi Kasei Pharma and was used to modify the electrode of the biosensor. Osmium-wired horseradish peroxidase (002096, Bioanalytical Systems, West Lafayette, IN, USA) was purchased from BAS Inc., Tokyo, Japan. Phosphate-buffered saline (PBS, pH 7.4, 50 mM (PO₄)) was prepared in the laboratory. Ultrapure water obtained from the water purification system (Direct-Q, Merck, Darmstadt, Germany) was used for the preparation of all aqueous solutions.

Konno S, & Kudo H. (2024). Fundamental Study of a Wristwatch Sweat Lactic Acid Monitor. Biosensors, 14(4), 187.

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Analytical-Grade Chemical Compounds

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Propionic acid (PA), butyric acid (BA), L-glycine (Gly), L-alanine (Ala), L-serine (Ser), L-threonine (Thr), L-cysteine (Cys), L-methionine (Met), L-valine (Val), L-leucine (Leu), L-isoleucine (Ile), L-phenylalanine (Phe), L-tryptophan (Trp), L-aspartic acid (Asp), L-glutamic acid (Glu), L-asparagine (Asn), L-glutamine (Glu), L-histidine (His), L-asparagine monohydrate (Arg), L-tyrosine (Tyr), and L-ascorbic acid were purchased from FUJIFILM Wako (Osaka, Japan). Acetic acid (AA), L-lysine (Lys), and sodium butyrate were purchased from Nacalai Tesque (Kyoto, Japan), and proline (Pro) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were of analytical grade.

Takakuwa A., Nakamura K., Kikuchi M., Sugimoto R., Ohira S., Yokoi Y, & Ayabe T. (2019). Butyric Acid and Leucine Induce α-Defensin Secretion from Small Intestinal Paneth Cells. Nutrients, 11(11), 2817.

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Formulation and Characterization of VD3

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Materials used in this study were purchased from the following commercial vendors: VD3 from Cayman Chemical (Ann Arbor, MI, USA). Excipients (special grade)—L-alanine (Ala), L-methionine (Met), L-phenylalanine (Phe), L-leucine (Leu), L-valine (Val) and L-isoleucine (Ile)—and reagents—ethanol (99.5%) (reagent grade), acetonitrile (HPLC grade) and polyoxyethylene (20) sorbitan monolaurate (biochemistry grade)—from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). 2 mL VIST glass vials obtained from Daiwa Special Glass (Osaka, Japan) and rubber stoppers (F5-43) obtained from Sumitomo Rubber Industries (Hyogo, Japan) were used as the packing materials.

Miyamoto K., Yanagisawa M., Taga H., Yamaji H., Akita T, & Yamashita C. (2021). Optimization of Very Low-Dose Formulation of Vitamin D3 with Lyophilizate for Dry Powder Inhalation System by Simple Method Based on Time-of-Flight Theory. Pharmaceutics, 13(5), 632.

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Amino Acid Reagent Preparation

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MEM essential amino acids solution, MEM non-essential amino acids solution, l-cystine, l-arginine, l-histidine, l-Isoleucine, l-leucine, l-lysine, l-methionine, l-phenylalanine, l-threonine, l-tryptophan, l-tyrosine, l-valine, William's Medium E (WME), Dulbecco's modified Eagle's medium (DMEM), and hanks' balanced salt solution (HBSS), were purchased from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Proteinase inhibitor cocktail was purchased from Nacalai Tesque, (Kyoto, Japan). Na₂S₄ was purchased from Dojindo Molecular Technologies, Inc (Kumamoto, Japan). β-(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM) was purchased from Molecular Biosciences (CO, USA). All other reagents and chemicals were of the highest grades available.

Akiyama M., Unoki T., Aoki H., Nishimura A., Shinkai Y., Warabi E., Nishiyama K., Furumoto Y., Anzai N., Akaike T., Nishida M, & Kumagai Y. (2022). Cystine-dependent antiporters buffer against excess intracellular reactive sulfur species-induced stress. Redox Biology, 57, 102514.

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Preparation of Solid Standard Materials

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L-Valine (Fujifilm Wako Pure Chemical Corporation, Tokyo, Japan), caffeine, anhydrous (Fujifilm Wako Pure Chemical Corporation, Tokyo, Japan), BBP (Sigma Aldrich, St. Louis, MO, USA), titanium(IV) oxide (Anatase Form, Kanto Chemical Co., Inc., Tokyo, Japan), calcium carbonate (Kanto Chemical Co., Inc., Tokyo, Japan), ethanol (Fujifilm Wako Pure Chemical Corporation, Tokyo, Japan), methanol (Fujifilm Wako Pure Chemical Corporation, Tokyo, Japan), formic acid (Fujifilm Wako Pure Chemical Corporation, Tokyo, Japan), photocurable resin (Acryl One #2100N, Maruto Instrument Co., Ltd., Tokyo, Japan) were used for preparation of the solid standard materials.

Hirata T., Yang M, & Hsin Khoo H. (2023). Direct Analysis of Bio-Molecules in Solid Materials Using Electrospray Ionisation Mass Spectrometry Coupled with Laser Ablation and a Liquid Sampling Technique. Mass Spectrometry, 12(1), A0121.

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Derivatization and HPLC Analysis of Amino Acids

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Amino acids (L-serine, L-alanine, L-valine, and L-proline), sodium hydroxide, sodium tetraborate, citric acid, sodium perchlorate monohydrate and diethylamine were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was obtained from Dojindo Laboratories (Kumamoto, Japan). Chloro(dimethyl)octadecylsilane, 95% (ODS-Cl), was obtained from Sigma-Aldrich (St. Louis, MO). Toluene and acetonitrile were of HPLC grade and were obtained from the Kanto Chemical Co., Inc. (Tokyo, Japan). Water was purified using a Milli-Q system (Millipore, Bedford, MA).

Smirnova A., Shimizu H., Mawatari K, & Kitamori T. (2015). Reversed-phase Chromatography in an Extended Nanospace: Separating Amino Acids in Short and Long Nanochannels. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 31(11).

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