Water oasis hlb columns

Manufactured by Waters Corporation

The Water Oasis HLB columns are solid-phase extraction (SPE) columns designed for sample preparation in analytical workflows. These columns utilize hydrophilic-lipophilic balanced (HLB) sorbent material to facilitate the extraction and purification of a wide range of analytes from various sample matrices.

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Lab products found in correlation

2 protocols using water oasis hlb columns

Proteome Analysis of Human Liver Microsomes

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Human liver microsomes (pooled from 200 donors with mixed-gender) were purchased form Sekisui XenoTech (Kansas City, KS). Dulbecco’s Modified Eagle Medium (DMEM), Fetal bovine serum (FBS), and 100× antibiotics mixture containing 100 IU/mL penicillin and 100 μg/mL streptomycin (P/S) were products of Invitrogen (Carlsbad, CA, USA). HyClone™ RPMI-1640 medium was purchased from Thermo Scientific (Waltham, MA). Urea and dithiothreitol were purchased from Fisher Scientific Co. (Pittsburgh, PA). Trifluoroacetic acid, formic acid, and acetonitrile were from Sigma-Aldrich (St. Louis, MO). Iodoacetamide and ammonium bicarbonate were the products of Acros Organics (Morris Plains, NJ). TPCK-treated trypsin was obtained from Worthington Biochemical Corporation (Freehold, NJ). Water Oasis HLB columns were from Waters Corporation (Milford, MA). Bovine serum albumin (BSA) standard was purchased from Thermo Fisher Scientific (Waltham, MA).

Shi J., Wang X., Lyu L., Jiang H, & Zhu H.J. (2018). Comparison of Protein Expressions between Human Livers and the Hepatic Cell Lines HepG2, Hep3B and Huh7 using SWATH and MRM-HR Proteomics: Focusing on Drug-Metabolizing Enzymes. Drug metabolism and pharmacokinetics, 33(2), 133-140.

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Quantification of Trandolapril and Metabolite

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Trandolapril and trandolaprilat were purchased from Cayman Chemical. Trandolapril‐phenyl‐d5 and trandolaprilat‐phenyl‐d5 were products of Toronto Research Chemicals. Blank human plasma was obtained from Innovative Research. Water Oasis HLB columns were purchased from Waters Corporation. Taq polymerase was obtained from New England Biolabs. All other chemicals and agents were of the highest analytical grade commercially available.
A total of 102 individual normal human liver samples were obtained from XenoTech LLC and the Cooperative Human Tissue Network. The donors comprised 46 men and 56 women with ages ranging from 22 to 81 years, and included 92 Whites, 6 African Americans, 2 Hispanics, and 2 classified as other.

Wang X., Her L., Xiao J., Shi J., Wu A.H., Bleske B.E, & Zhu H. (2021). Impact of carboxylesterase 1 genetic polymorphism on trandolapril activation in human liver and the pharmacokinetics and pharmacodynamics in healthy volunteers. Clinical and Translational Science, 14(4), 1380-1389.

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