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Cobas mira chemistry analyzer

Manufactured by Roche
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The Cobas-Mira Chemistry Analyzer is a compact, automated clinical chemistry analyzer. It is designed to perform a variety of routine clinical chemistry tests on patient samples. The Cobas-Mira can analyze different types of samples, including serum, plasma, and urine. It is capable of processing multiple samples simultaneously and provides fast, reliable results.

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Lab products found in correlation

Human igfbp 3 quantikine elisa, by R&D Systems (2 mentions) Human il 6 quantikine hs elisa, by R&D Systems (2 mentions) Module p, by Roche (2 mentions) Human igf 1 quantikine elisa, by R&D Systems (2 mentions) 2000 autoanalyzer, by Tosoh (2 mentions) Enzychrom triglyceride assay kit, by BioAssay Systems (1 mentions) Cobas 6000 analyzer, by Roche (1 mentions) Insulin ria kit, by Linco Research (1 mentions)

Close spelling variants of this product

COBAS Mira S chemistry analyzer COBAS MIRA Plus Chemistry Analyzer Cobas Mira analyzer Cobas Mira Roche Chemistry Analyzers Cobas analyzer

6 protocols using cobas mira chemistry analyzer

1

Serum Biomarker Measurement Protocol

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Mice were fasted for 16 h, anaesthetized with isoflurane and blood was collected by cardiac puncture and kept for 30 min at room temperature to promote clot formation. Serum was prepared by cold centrifugation at 3000g for 20 min. Serum was stored at −20° C until use. Serum glucose was determined using routine methods using commercial reagents (Roche) on a Cobas 6000 analyzer (Roche). Fructosamine levels were determined on a Cobas-Mira Chemistry Analyzer using the Roche reagents (Roche). Triglyceride levels were determined with the EnzyChrom Triglyceride Assay Kit (BioAssay Systems) according to the manufacturer's instructions.
Thoonen R., Cauwels A., Decaluwe K., Geschka S., Tainsh R.E., Delanghe J., Hochepied T., De Cauwer L., Rogge E., Voet S., Sips P., Karas R.H., Bloch K.D., Vuylsteke M., Stasch J.P., Van de Voorde J., Buys E.S, & Brouckaert P. (2015). Cardiovascular and pharmacological implications of haem-deficient NO-unresponsive soluble guanylate cyclase knock-in mice. Nature Communications, 6, 8482.
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2

Biomarker Measurement and Analysis

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Blood was collected on the first day and last day of each intervention period after a 12–hour fast by trained research staff. Specimens were locally processed and stored at −80°C until analyses. Glucose was measured on a Roche Module P chemistry autoanalyzer (Roche Diagnostic Inc., Indianapolis, IN) at the Northwest Lipid Research Laboratories (University of Washington, WA). Insulin was measured using a Tosoh 2000 autoanalyzer (Tosoh Biosciences Inc., South San Francisco, CA) at the Diabetes Endocrinology Research Center Immunoassay Laboratory (University of Washington, WA). The rest of the biomarkers assessments and the genotyping were conducted at the FHCRC Biomarker Core Laboratory and the Molecular Epidemiology Laboratories. Immunoassays were used to measured total adiponectin (Total Adiponectin EIA, Aplco), IGF-1 (Human IGF-I Quantikine ELISA, R&D Systems), IGFBP-3 (Human IGFBP-3 Quantikine ELISA, R&D Systems), and IL-6 (Human IL-6 Quantikine HS ELISA, R&D Systems). CRP was measured using CRP (3 (link))-Wide Range reagent (Kamiya Biomedical Company) on Roche Cobas Mira chemistry analyzer with a high sensitivity protocol. The intra-assay CVs were 0.7%, 7.8%, 1.3%, 1.5%, 1.8%, 2.3%, and 3.3% for glucose, insulin, adiponectin, IGF-1, IGFBP-3, IL-6, and CRP, respectively. The details of specimen collection and analysis have been previously described (14 (link)).
Santiago-Torres M., De Dieu Tapsoba J., Kratz M., Lampe J.W., Breymeyer K.L., Levy L., Song X., Villaseñor A., Wang C.Y., Fejerman L., Neuhouser M.L, & Carlson C.S. (2016). Genetic ancestry in relation to the metabolic response to a U.S. versus traditional Mexican diet: a randomized crossover feeding trial among women of Mexican descent. European journal of clinical nutrition, 71(3), 395-401.
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3

Biomarker Measurement and Analysis

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Blood was collected on the first day and last day of each intervention period after a 12–hour fast by trained research staff. Specimens were locally processed and stored at −80°C until analyses. Glucose was measured on a Roche Module P chemistry autoanalyzer (Roche Diagnostic Inc., Indianapolis, IN) at the Northwest Lipid Research Laboratories (University of Washington, WA). Insulin was measured using a Tosoh 2000 autoanalyzer (Tosoh Biosciences Inc., South San Francisco, CA) at the Diabetes Endocrinology Research Center Immunoassay Laboratory (University of Washington, WA). The rest of the biomarkers assessments and the genotyping were conducted at the FHCRC Biomarker Core Laboratory and the Molecular Epidemiology Laboratories. Immunoassays were used to measured total adiponectin (Total Adiponectin EIA, Aplco), IGF-1 (Human IGF-I Quantikine ELISA, R&D Systems), IGFBP-3 (Human IGFBP-3 Quantikine ELISA, R&D Systems), and IL-6 (Human IL-6 Quantikine HS ELISA, R&D Systems). CRP was measured using CRP (3 (link))-Wide Range reagent (Kamiya Biomedical Company) on Roche Cobas Mira chemistry analyzer with a high sensitivity protocol. The intra-assay CVs were 0.7%, 7.8%, 1.3%, 1.5%, 1.8%, 2.3%, and 3.3% for glucose, insulin, adiponectin, IGF-1, IGFBP-3, IL-6, and CRP, respectively. The details of specimen collection and analysis have been previously described (14 (link)).
Santiago-Torres M., De Dieu Tapsoba J., Kratz M., Lampe J.W., Breymeyer K.L., Levy L., Song X., Villaseñor A., Wang C.Y., Fejerman L., Neuhouser M.L, & Carlson C.S. (2016). Genetic ancestry in relation to the metabolic response to a U.S. versus traditional Mexican diet: a randomized crossover feeding trial among women of Mexican descent. European journal of clinical nutrition, 71(3), 395-401.
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4

Metabolic Biomarkers in Juvenile Rats

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At 9 months of age, one male from each litter was fasted overnight and blood was collected via cardiac puncture in heparinized tubes for analysis. Plasma insulin was measured using rat specific commercial radioimmunoassay kits (insulin RIA kit, LINCO Research Inc., St. Charles, MO). Plasma triglycerides were measured using Raichem Enzymatic Reagents (Cat No. 80008, Raichem, Inc., San Diego, CA) with control serum level 1 (#83082) and control serum level 2 (#83083) and run on an automated Cobas-Mira Chemistry Analyzer (Roche Diagnostic Systems Inc., Sommerville, NJ). Blood glucose determined using Hemocue B-Glucose Analyzer (HemoCue Inc, Mission Viejo, CA).
Desai M., Jellyman J.K., Han G., Lane R.H, & Ross M.G. (2015). Programmed Regulation of Rat Offspring Adipogenic Transcription Factor (PPARγ) by Maternal Nutrition. Journal of developmental origins of health and disease, 6(6), 530-538.
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5

Diabetes Ascertainment in Aging Cohort

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At every study visit, diabetes classification was based on fasting glucose level ≥126 mg/dL, anti-diabetic medication use, or self-report of a physician diagnosis of diabetes at the baseline examination. Fasting glucose was measured with the Cobas Mira Chemistry Analyzer (Roche Diagnostics Corporation, Indianapolis, IN) and medication use was ascertained by inspection of medications. The majority (75.5%) of participants with diabetes at baseline met at least two criteria and 24.5% met one (10.8% fasting glucose level, 2.1% medication use, and 11.7% self-report). Due to the advanced age of the cohort, most diabetes cases are likely to be type 2 diabetes. At each wave, individuals were designated as having one of the following: baseline diabetes, diabetes at the current wave that was diagnosed since baseline (diabetes diagnosed during study), or no diabetes.
Mayeda E.R., Haan M.N., Yaffe K., Kanaya A.M, & Neuhaus J. (2015). Does type 2 diabetes increase rate of cognitive decline in older Mexican Americans?. Alzheimer disease and associated disorders, 29(3), 206-212.
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6

Plasma Hormone and Lipid Profiling

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Plasma insulin, leptin and coritcosterone levels were measured using rat specific commercial radioimmunoassay kits (insulin and leptin RIA kit, LINCO Research Inc., St. Charles, MO; coritcosterone RIA Kit, Diagnostic Systems Laboratories, Inc, Webster, TX). Plasma lipid levels were measured using reagents from Raichem, Inc. (San Diego, CA) and run on an automated Cobas-Mira Chemistry Analyzer (Roche Diagnostic Systems Inc., Sommerville, NJ). Plasma triglycerides (Cat No. 80008) and cholesterol (Cat No. 80015) concentrations were analyzed using Raichem Enzymatic Reagents (with control serum level 1 #83082 and control serum level 2 #83083). Blood glucose determined using Hemocue B-Glucose Analyzer (HemoCue Inc, Mission Viejo, CA).
DESAI M., JELLYMAN J.K., HAN G., BEALL M., LANE R.H, & ROSS M.G. (2014). Rat Maternal Obesity and High Fat Diet Program Offspring Metabolic Syndrome. American journal of obstetrics and gynecology, 211(3), 237.e1-237.e13.
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