Breast cancer cell lines MDA‐MB‐231 (RRID: CVCL_0062), MCF‐7 (RRID: CVCL_0031) and BT‐549 (RRID: CVCL_1092) were obtained from American Type Culture Collection (ATCC, Manassas, VA). Hair‐follicle‐derived stem cell and dermal fibroblast were isolated as described previously [27 (link)]. All cells except BT‐549 were cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 1% (v/v) antibiotic‐antimycotic cocktail (Thermo Fisher Scientific, Grand Island, NY, USA) and 10% (v/v) fetal bovine serum (Atlanta Biologicals, Norcross, GA, USA). BT‐549 was cultured in RPMI (Thermo Fisher Scientific) supplemented with 1% (v/v) antibiotic‐antimycotic cocktail, 10% (v/v) fetal bovine serum and 0.023 U·mL−1 insulin. All cell lines have been authenticated in the past 3 years by STR profiling at ATCC. According to the vendor, the commercially available PowerPlex® 18D kit (Promega, Madison, WI, USA) was used to amplify 17 short tandem repeat (STR) loci plus the gender‐determining locus, Amelogenin. Samples were then processed with the ABI Prism® 3500xl Genetic Analyzer (Thermo Fisher Scientific), and data were analyzed using GeneMapper® ID‐X software (Thermo Fisher Scientific) to confirm cell identity and purity. Finally, all experiments were performed with mycoplasma‐free cells.
Antibiotic antimycotic cocktail
Manufactured by Thermo Fisher Scientific
Sourced in United States
Antibiotic-antimycotic cocktail is a liquid solution containing a combination of antibiotics and antifungal agents. It is commonly used in cell culture applications to prevent bacterial and fungal contamination. The solution typically includes penicillin, streptomycin, and amphotericin B as the active ingredients.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (14 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (6 mentions)
Streptomycin, by Thermo Fisher Scientific (5 mentions)
Amphotericin b, by Thermo Fisher Scientific (5 mentions)
Mcf 7, by American Type Culture Collection (4 mentions)
Mda mb 231, by American Type Culture Collection (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Epidermal growth factor (egf), by Merck Group (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Hydrocortisone, by Merck Group (2 mentions)
Antibiotic antimycotic cocktail, by Merck Group (2 mentions)
Hem cells, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Corning (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Alpha mem, by Thermo Fisher Scientific (2 mentions)
Cholera toxin, by Merck Group (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Hygromycin, by Merck Group (2 mentions)
Blasticidin s hcl, by Thermo Fisher Scientific (2 mentions)
Effectene transfection reagent, by Qiagen (2 mentions)
44 protocols using antibiotic antimycotic cocktail
1
Cell Line Authentication and Mycoplasma-Free Culture
2
Osteoblast Differentiation on Collagen Matrix
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MC3T3-E1 mouse osteoblast-like cell line was utilized in this study. Cells were cultured in MEM-α (Gibco, USA) containing 10% fetal bovine serum (Gibco) and 1% antibiotic–antimycotic cocktail (Gibco) in a humidified incubator at 37 °C. Cell passage was carried out when the confluency of the cells reached up to 80–90%. The cells from passage 25 to 30 were utilized for the experiment. We subcultured the cells on the collagen matrix using a cell density of 10,000 cells/cm2 in an osteogenic induction medium containing 50 μg/mL ascorbic acid and 10 mM β-glycerophosphate. The osteogenic induction medium was changed every 2 days when culturing cells on the collagen matrix for 4 or 10 days.
3
Photodynamic Therapy with Diiodo-Squaraine Sensitizer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human breast cell lines (MDA-MB-231, MCF7 and MCF 10A), colorectal cancer cells (HCT-116), cervical cancer cells (HeLa and SiHa), and pancreatic cancer cells (MIAPaCa-2) were purchased from ATCC (USA), and human oral cancer cells (SCC-131 and SCC-172) were obtained as a gift from Dr. Susanne M Gollin, University of Pittsburgh, USA, and were maintained in DMEM(Sigma, USA) containing 10% fetal bovine serum (Gibco, USA) and 1% antibiotic antimycotic cocktail (Gibco, USA). The diiodo-squaraine photosensitizer was synthesized as previously described20 (link)51 (link)52 , and 100 mm stock solution in DMSO was prepared for further studies. The light source was a 700 mW–630 nM laser with optical fiber system (Vinvish LTD, India).
4
Isolation and Culture of HMSCs
5
Establishment of Primary Tumor Organoids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The primary tumors from different genotypes of GEMMs were mechanically sliced into small pieces smaller (< 1 mm3) using scalpels and then dissociated with collagenase and hydrogenase. The resultant organoids of the tumors were collected and cultured on the collagen I (EMD Millipore)-coated six-well plates. The primary tumor cells were cultured in DMEM/F12 Ham’s medium supplemented with 5% horse serum (Life Technologies), 10 μg ml−1 insulin (Invitrogen), 20 ng ml−1 EGF (Sigma), 100 ng ml−1 cholera toxin (EMD Millipore), 0.5 mg ml−1 hydrocortisone (Sigma), and antibiotic-antimycotic cocktail (Gibco).
6
Melanoma and Melanocyte Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
B16-F0 (ATCC, CRL-6322), a murine melanoma cell line, was seeded in DMEM (Sigma-Aldrich, D2902) supplemented with 10% FBS (Corning, 35-015-CV) and antibiotic-antimycotic cocktail (GIBCO, 15240062), and incubated under an atmosphere of 5% CO2 at 37 °C. Melan-a (a gift from D. C. Bennet at St George's University of London), a murine melanocyte cell line, was cultured in RPMI 1640 (GIBCO, 31800089) supplemented with 10% FBS, antibiotic-antimycotic cocktail and 200 nM 12-O-tetradecanoylphorbol 13-acetate (Sigma-Aldrich, P8139) under an atmosphere of 5% CO2 at 37 °C.
7
Culturing NCI/ADR-RES Cell Line
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NCI/ADR-RES cell line was obtained from National Cancer Institute (NCI, USA). The cells were maintained in DMEM (Sigma, USA) containing 10% heat inactivated FBS (GIBCO, USA) and 1% antibiotic-antimycotic cocktail (GIBCO, USA).
8
Cultivation of Melanocyte and Melanoma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEM cells (Thermo Fischer Scientific, C1025C) were cultured in medium 254 (GIBCO, M254) containing human melanocyte growth supplement (GIBCO, S002). B16-F0 mouse melanoma cell line (ATCC, CRL-6322) was cultured in DMEM (Sigma-Aldrich, D2902) containing 10% fetal bovine serum (FBS, Corning, 35-015-CV) and antibiotic-antimycotic cocktail (GIBCO, 15240062). MNT1 human melanoma cell line (a gift from Dr. E.-S. Oh at Ewha Womans University) was cultured in MEM (Welgene, LM007-7) containing 10% DMEM, 20% FBS, 20 mM HEPES (Sigma-Aldrich, H4034) and antibiotic-antimycotic cocktail. The cells were incubated under an atmosphere of 5% CO2 at 37℃.
9
Prostate Cell Line Culture and Treatments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The normal human prostatic epithelial cell line, RWPE-1, and normal human prostatic stromal cell WPMY-1 were acquired from the American Type Culture Collection (Manassas, VA, USA). RWPE-1 cells were cultured in Keratinocyte Serum-Free Medium supplemented with 0.05 mg/mL bovine pituitary extract, 5 ng/mL human recombinant epidermal growth factor, and an antibiotic-antimycotic cocktail (Gibco, Grand Island, NY, USA). WPMY-1 cells were cultured in Dulbecco’s Modified Eagle’s Medium, supplemented with 1% penicillin/streptomycin and 10% FBS (Gibco). After 24 h of incubation, the cells were serum starved prior to some of the experiments, as indicated. Then, the cells were treated with 10 nM DHT for 24–72 h, with or without various concentrations of components from HBX-5 (0.25–1000 μg/mL).
10
Isolation of Adipose-Derived Mesenchymal Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human subcutaneous and visceral adipose tissues were available as surgical excess following elective abdominoplasty at St Vincent’s Private Hospital (Melbourne, Australia). Cells were isolated from these tissues using a protocol previously described by Zuk et al.28 (link), with modifications. Briefly, adipose tissue was mechanically minced into fine pieces and cells were dissociated using an enzymatic digestion (0.2% w/v) collagenase 1 for 60 min at 37°C. Cells were washed, centrifuged to remove adipocytes and pelleted cells were cultured under standard conditions in low glucose DMEM containing 5 mM glucose, 2 mM L-glutamine (Sigma) and 100 U/ml antibiotic/antimycotic cocktail (Gibco) at 37°C in a humidified atmosphere containing 5% CO2. Adherent populations were grown until they became confluent under standard conditions, and sub-cultured at a ratio of 1:2. The cultured populations of cells were termed adipose-derived mesenchymal cells (AMCs).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!