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Peroxidase conjugated anti rabbit secondary antibody a 4914

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Peroxidase-conjugated anti-rabbit secondary antibody (A-4914) is a laboratory reagent used in immunoassays and other immunochemical techniques. It is designed to detect and bind to primary antibodies raised in rabbits, allowing for the visualization and identification of target antigens.

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Lab products found in correlation

Peroxidase conjugated anti mouse secondary antibody a 4416, by Merck Group (12 mentions) Monoclonal anti ha antibody mms 101p, by Fortrea (6 mentions) Monoclonal anti ha antibody mms 101p, by BioLegend (4 mentions) Anti vinculin antibody v 4505, by Merck Group (4 mentions) Polyclonal anti ha antibody sc 805, by Santa Cruz Biotechnology (4 mentions) Anti p27 sc 528, by Santa Cruz Biotechnology (3 mentions) Anti ha agarose beads a2095, by Merck Group (3 mentions) Anti gfp antibody 632380, by Thermo Fisher Scientific (3 mentions) Monoclonal anti flag antibody f3165, by Merck Group (3 mentions) Anti brd3 11859 1 ap antibody, by Proteintech (3 mentions) Anti β trcp1 4394, by Cell Signaling Technology (2 mentions) Polyclonal anti flag antibody f 2425, by Merck Group (2 mentions) Anti brd4 antibody, by Cell Signaling Technology (2 mentions) A301 985a m, by Fortis Life Sciences (2 mentions) Anti gfp 8371 2 antibody, by Takara Bio (2 mentions) Erg antibody epr3864 2, by Abcam (2 mentions) Polyclonal anti myc tag antibody 2278, by Cell Signaling Technology (2 mentions) Monoclonal anti myc tag 2276 antibodies, by Cell Signaling Technology (2 mentions) Polyclonal anti ha sc 805, by Santa Cruz Biotechnology (2 mentions) Anti xpress 46 0528 antibody, by Thermo Fisher Scientific (2 mentions)

17 protocols using peroxidase conjugated anti rabbit secondary antibody a 4914

1

Antibody Characterization for Protein Analysis

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-PDK1 antibody (8292787) were purchased from BD Biosciences. Anti-SPOP antibody (16750–1-AP) and anti-CK1 antibody (14388–1-AP) were purchased from Proteintech. Anti-pS473-AKT antibody (4060), anti-pT308-AKT antibody (2965), anti-AKT1 antibody (2938), anti-AKT total antibody (4691), anti-Cullin 3 (2759), anti-GST (2625), polyclonal anti-Myc-Tag antibody (2278), anti-GAPDH (5174) and monoclonal anti-Myc-Tag (2276) antibodies were purchased from Cell Signaling Technology. Monoclonal anti-HA antibody (MMS-101P) was purchased from Biolegend. Anti-TRIM24 (C-4), polyclonal anti-HA (SC-805) and anti-p27 (SC-528) were purchased from Santa Cruz. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma.
Jiang Q., Zheng N., Bu L., Zhang X., Zhang X., Wu Y., Su Y., Wang L., Zhang X., Ren S., Dai X., Wu D., Xie W., Wei W., Zhu Y, & Guo J. (2021). SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions. Molecular Cancer, 20, 100.
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2

Immunoblot Analysis of Ubiquitin-Proteasome Pathway

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Anti-Set8 (C18B7; 1:1,000 immunoblot (IB)), anti-β-catenin (D10A8; 1:1,000 IB), anti-Skp2 (D3G5; 1:1,000 IB), anti-Mcl-1 (D35A5; 1:1,000 IB), anti-G9A (C6H3; 1:1,000 IB), anti-Cullin 1 (4995; 1:1,000 IB) and anti-β-TRCP1 (4394; 1:1,000 IB) antibodies were purchased from Cell Signaling Technology. Anti-p27 (SC-528; 1:500 IB), anti-Cullin 1 (SC-17775; 1:1,000 IB), anti-casein kinase 1δ (H-60; 1:1,000 IB), anti-casein kinase 1α (SC-6474; 1:1,000 IB), anti-c-Myc (9E10; 1:1,000 IB, 2 μg immunoprecipitates (IP)) and polyclonal anti-HA antibodies (SC-805; 1:1,000 IB, 2 μg IP) were purchased from Santa Cruz Biotechnology. Anti-Vinculin antibody (V-4505; 1:2,000 IB), monoclonal anti-Flag antibody (F-3165), polyclonal anti-Flag antibody (F-2425; 1:1,000 IB, 2 μg IP), anti-HA agarose beads (A-2095; 1:1,000 IB, 2 μg IP), peroxidase-conjugated anti-mouse secondary antibody (A-4416; 1:3,000 IB) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914; 1:3,000 IB) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Covance. Anti-GFP antibody (632380; 1:1,000 IB), anti-Cdh1 (34-2000; 1:1,000 IB) antibody, Oligofectamine, Lipofectamine and Plus reagents were purchased from Invitrogen. Anti-CUL 4B (16184-1-AP; 1:1,000 IB) antibody was purchased from Proteintech Group.
Wang Z., Dai X., Zhong J., Inuzuka H., Wan L., Li X., Wang L., Ye X., Sun L., Gao D., Zou L, & Wei W. (2015). SCFβ-TRCP promotes cell growth by targeting PR-Set7/Set8 for degradation. Nature Communications, 6, 10185.
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3

Comprehensive Antibody Validation Protocol

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-ERG antibody [EPR3864 (2)] and (C-20) were purchased from Abcam and Santa Cruz, respectively. Anti-SPOP antibody (16750-1-AP), Anti-BRD2 (22236-1-AP) and anti-BRD3 (11859-1-AP) antibody were purchased from Proteintech. Anti-BRD4 antibody was purchased from Cell Signaling (13440) and Bethyl laboratories (A301-985A-M). Anti-Cullin 3 (2759), anti-GST (2625), anti-DEK (13962s), anti-SRC3 (2126), anti-Cleaved Caspase-3 (9664), anti-Cleaved PARP (5625), polyclonal anti-Myc-Tag antibody (2278) and monoclonal anti-Myc-Tag (2276) antibodies were purchased from Cell Signaling. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Biolegend. Anti-GFP (8371-2) antibody was purchased from Clontech. Anti-Cullin 1 (SC-17775), anti-AR (N-20), anti-TRIM24/TIF1α (C-4), polyclonal anti-HA (SC-805) and anti-p27 (SC-528) were purchased from Santa Cruz. Anti-Xpress (46-0528) antibody was purchased from Invitrogen.
Dai X., Gan W., Li X., Wang S., Zhang W., Huang L., Liu S., Zhong Q., Guo J., Zhang J., Chen T., Shimizu K., Beca F., Blattner M., Vasudevan D., Buckley D.L., Qi J., Buser L., Liu P., Inuzuka H., Beck A.H., Wang L., Wild P.J., Garraway L.A., Rubin M.A., Barbieri C.E., Wong K.K., Muthuswamy S.K., Huang J., Chen Y., Bradner J.E, & Wei W. (2017). Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4. Nature medicine, 23(9), 1063-1071.
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4

Western Blot Analysis of Protein Samples

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Cells were lysed in EBC buffer supplemented with protease inhibitors cocktail and phosphatase inhibitors, and the protein concentrations were measured as described above. The lysates (40 μg protein) were then resolved by 10% SDS-PAGE at 130 V for 80 min, immunoblotted with indicated antibodies at 4 °C overnight, washed 4 times with Tris-buffered saline with 0.1% Tween-20 (TBST), incubated with secondary antibody for 1 h at room temperature, and then washed 4 times with TBST buffer. Anti-p65 (#8742, 1:1000) and E2F1 (#3742, 1:500) antibodies were purchased from Cell Signaling Technologies. Anti-HA antibody (#901513, 1:1000) was purchased from Biolegend. Anti-Flag (F3165, 1: 5000), antivinculin antibody (V-4505, 1: 50000), peroxidase-conjugated antimouse secondary antibody (A-4416, 1:3000), and peroxidase-conjugated antirabbit secondary antibody (A-4914, 1:3000) were purchased from Sigma. All primary antibodies were diluted in 5% bovine serum albumin (BSA) in TBST buffer, and secondary antibodies were diluted in 5% nonfat milk in TBST buffer.
Liu J., Chen H., Kaniskan H.Ü., Xie L., Chen X., Jin J, & Wei W. (2021). TF-PROTACs Enable Targeted Degradation of Transcription Factors. Journal of the American Chemical Society, 143(23), 8902-8910.
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5

Antibody Dilution and Sourcing

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All the primary antibodies were used at a 1:1000 dilution, and secondary antibodies were diluted at 1:3000 dilution in 5% nonfat milk for immunoblotting assay. Anti-BAP1 (sc-28383), anti-N-Myc (sc-53993) and Polyclonal anti-HA (Y-11) were purchased from Santa Cruz Biotechnology. Anti-His (2365 S) antibodies were purchased from Cell Signaling. Monoclonal anti-HA antibody (MMS-101P) was purchased from Covance. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma.
Zhang X., Cong X., Jin X., Liu Y., Zhang T., Fan X., Shi X., Zhang X., Wang X., Yang Y.G, & Dai X. (2023). Deficiency of BAP1 inhibits neuroblastoma tumorigenesis through destabilization of MYCN. Cell Death & Disease, 14(8), 504.
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6

Comprehensive Antibody Validation Protocol

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-ERG antibody [EPR3864 (2)] and (C-20) were purchased from Abcam and Santa Cruz, respectively. Anti-SPOP antibody (16750-1-AP), Anti-BRD2 (22236-1-AP) and anti-BRD3 (11859-1-AP) antibody were purchased from Proteintech. Anti-BRD4 antibody was purchased from Cell Signaling (13440) and Bethyl laboratories (A301-985A-M). Anti-Cullin 3 (2759), anti-GST (2625), anti-DEK (13962s), anti-SRC3 (2126), anti-Cleaved Caspase-3 (9664), anti-Cleaved PARP (5625), polyclonal anti-Myc-Tag antibody (2278) and monoclonal anti-Myc-Tag (2276) antibodies were purchased from Cell Signaling. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Biolegend. Anti-GFP (8371-2) antibody was purchased from Clontech. Anti-Cullin 1 (SC-17775), anti-AR (N-20), anti-TRIM24/TIF1α (C-4), polyclonal anti-HA (SC-805) and anti-p27 (SC-528) were purchased from Santa Cruz. Anti-Xpress (46-0528) antibody was purchased from Invitrogen.
Dai X., Gan W., Li X., Wang S., Zhang W., Huang L., Liu S., Zhong Q., Guo J., Zhang J., Chen T., Shimizu K., Beca F., Blattner M., Vasudevan D., Buckley D.L., Qi J., Buser L., Liu P., Inuzuka H., Beck A.H., Wang L., Wild P.J., Garraway L.A., Rubin M.A., Barbieri C.E., Wong K.K., Muthuswamy S.K., Huang J., Chen Y., Bradner J.E, & Wei W. (2017). Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4. Nature medicine, 23(9), 1063-1071.
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7

Antibody Dilution and Specificity

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-EglN2 (NB100-310) and anti-EglN3 (NB100-139) antibodies were obtained from Novus biological. Anti-SPOP (16750-1-AP) antibody was purchased from Proteintech. Anti-EglN1 (4835), anti-Cullin 3 (2759), anti-GST (2625), anti-GFP (2956), anti-AR (5153) and anti-Myc-Tag (2278) antibodies were purchased from Cell Signaling. Polyclonal anti-HA (SC-805) antibody was purchased from Santa Cruz. Monoclonal anti-Flag (F-3165, clone M2), anti-Tubulin (T-5168), anti-Vinculin (V-4505) antibodies, anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Monoclonal anti-HA (MMS-101P) antibody was purchased from Covance.
Zhang L., Peng S., Dai X., Gan W., Nie X., Wei W., Hu G, & Guo J. (2017). Tumor suppressor SPOP ubiquitinates and degrades EglN2 to compromise growth of prostate cancer cells. Cancer letters, 390, 11-20.
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8

Western Blot Antibody Validation

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Anti-IKZF1 (ab191394) antibody was purchased from Abcam. Anti-IKZF3 (NBP22449) antibody was purchased from Novus Biologicals. Anti-BRD3 (11859-1-AP) antibody was purchased from Proteintech. Anti-BRD4 (A301-985A-M) antibody was purchased from Bethyl Laboratories. Anti-ALK (3633) was purchased from Cell Signaling Technologies. Monoclonal anti-HA antibody (MMS-101P) was purchased from BioLegend. Polyclonal anti-HA (sc-805) antibody was purchased from Santa Cruz Biotechnologies. Anti-GFP antibody (632380) was purchased from Invitrogen. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-tubulin antibody (T-5168), anti-vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416), and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma-Aldrich. All antibodies were used at a 1:1000 dilution in 5% bovine serum albumin in tris-buffered saline and tween 20 (TBST) buffer for Western blots.
Liu J., Chen H., Ma L., He Z., Wang D., Liu Y., Lin Q., Zhang T., Gray N., Kaniskan H.Ü., Jin J, & Wei W. (2020). Light-induced control of protein destruction by opto-PROTAC. Science Advances, 6(8), eaay5154.
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9

Immunoprecipitation Assay with Diverse Antibodies

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Anti-Acetylated Lysine antibody (9441), anti-SKP2 antibody (2652), anti-p300 antibody (86377), anti-p27 antibody (3686), anti-Lamin A/C antibody (4777), anti-AKT1 antibody (2938), anti-phospho-AKT1 (Ser473) antibody (9018), anti-E-cadherin antibody (4065), and anti-SIRT3 antibody (2627) were purchased from Cell Signaling Technology. Agarose conjugated anti-p300 antibody (SC-584 AC), agarose conjugated anti-SKP2 antibody (SC-74477 AC), and anti-p21 antibody (SC-6246) were purchased from Santa Cruz. Anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), peroxidase-conjugated anti-mouse secondary antibody (A-4416), and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma-Aldrich. Monoclonal anti-HA antibody (MMS-101P) was purchased from Covance.
Rezaeian A.H., Phan L.M., Zhou X., Wei W, & Inuzuka H. (2023). Pharmacological inhibition of the SKP2/p300 signaling axis restricts castration-resistant prostate cancer. Neoplasia (New York, N.Y.), 38, 100890.
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10

Antibody Validation and Detection

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Anti-IKZF1 (ab191394, 1:1000) antibody was purchased from Abcam. Anti-IKZF3 (NBP22449, 1:1000) antibody was purchased from Novus Biologicals. Anti-ALK (3633, 1:1000) and anti-CRBN (71,810, 1:1000) were purchased from Cell Signaling Technologies. Anti-FOLR1 (23355–1-AP, 1:1000) antibodies was purchased from Proteintech. Monoclonal anti-Flag antibody (F-3165, 1:5000), anti-Vinculin antibody (V-4505; 1:50,000), peroxidase-conjugated anti-mouse secondary antibody (A-4416; 1:5000), and peroxidase-conjugated anti-rabbit secondary antibody (A-4914; 1:5000) were purchased from Sigma-Aldrich. All primary antibodies were prepared in 5% bovine serum albumin (BSA) in the TBST buffer and secondary antibodies were prepared in 5% nonfat milk in the TBST buffer.
Chen H., Liu J., Kaniskan H.Ü., Wei W, & Jin J. (2021). Folate-Guided Protein Degradation by Immunomodulatory Imide Drug-Based Molecular Glues and Proteolysis Targeting Chimeras. Journal of medicinal chemistry, 64(16), 12273-12285.
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