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Control igg

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Control IgG is a laboratory product that serves as a reference control for immunoglobulin G (IgG) quantification and detection assays. It provides a consistent and standardized sample for quality control purposes.

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Lab products found in correlation

Magna rip rna binding protein immunoprecipitation kit, by Merck Group (27 mentions) Ez magna rip kit, by Merck Group (26 mentions) Anti ago2, by Merck Group (8 mentions) Human anti ago2 antibody, by Merck Group (7 mentions) Chip assay kit, by Merck Group (4 mentions) Magnetic bead, by Merck Group (4 mentions) Nanodrop, by Thermo Fisher Scientific (4 mentions) Dna purification kit, by Qiagen (4 mentions) Ago2 antibody, by Abcam (3 mentions) Ez magna rna immunoprecipitation kit, by Merck Group (3 mentions) Ez magna rip rna binding protein immunoprecipitation kit, by Merck Group (3 mentions) Ago2 antibody, by Merck Group (3 mentions) Anti h3k4me3, by Merck Group (3 mentions) Anti h3k27me3, by Merck Group (3 mentions) Bioruptor, by Diagenode (3 mentions) Anti h3k4me3, by Cell Signaling Technology (3 mentions) Upstate, by Merck Group (3 mentions) H3k79me2, by Abcam (3 mentions) Anti h3, by Abcam (2 mentions) Dynabeads protein a, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Control rabbit IgG (40 citations) Control mouse IgG (40 citations) Isotype control IgG (14 citations) Control human IgG (5 citations) Normal mouse immunoglobulin G (IgG) control (4 citations) Anti-AGO2 antibody or control IgG (3 citations) Rabbit and mouse IgG isotype controls (2 citations) Isotype control human IgG (2 citations) Mouse IgG antibody (control) (2 citations) Control rat IgG antibody (2 citations)

149 protocols using control igg

1

Western Blotting of Cellular Proteins

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Proteins (30–100 µg) were extracted and analysed by western blotting as described53 (link). Primary antibodies used in this study were: anti-RBM14 (Euromedex, ref. 10196-1-AP), anti-PPARγ (Santa Cruz, ref. sc-7196), anti-TFIIB (Santa Cruz, ref. sc-225), anti-β-actin (Santa Cruz, ref. sc-1616), anti-H3 (Abcam, ref. ab1791) and control IgG (Merck-Millipore, ref. 17–658). Secondary antibodies were anti-rabbit IgG-peroxidase antibody (Sigma, ref. A0545) or anti-goat IgG-peroxidase antibody (Sigma, ref. A5420).
Firmin F.F., Oger F., Gheeraert C., Dubois-Chevalier J., Vercoutter-Edouart A.S., Alzaid F., Mazuy C., Dehondt H., Alexandre J., Derudas B., Dhalluin Q., Ploton M., Berthier A., Woitrain E., Lefebvre T., Venteclef N., Pattou F., Staels B., Eeckhoute J, & Lefebvre P. (2017). The RBM14/CoAA-interacting, long intergenic non-coding RNA Paral1 regulates adipogenesis and coactivates the nuclear receptor PPARγ. Scientific Reports, 7, 14087.
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2

Isolation and Analysis of T-bet and Runx1 in CD8+ T Cells

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CD8 T cells were isolated from LCMV-infected B6 mice on day 7 p.i. using Dynabeads FlowComp mouse CD8 kit. Nuclear lysates were prepared using the Active Motif Nuclear Complex Co-IP Kit (cat: 54001), according to the manufacturer’s protocol and subject to either 20 cycles of sonication on Diagenode Bioruptor (30sec on/30sec off on high) alone, or sonication followed by digestion with the Nuclear Complex Co-IP Kit enzymatic shearing cocktail. Protein concentrations were quantified using Pierce micro BCA kit (cat: 23235). Nuclear lysates were incubated with polyclonal antibodies against T-bet (Santa Cruz, cat: sc-21003x), Runx1 (Thermo Fischer, cat: PA5–19638), or control IgG (EMD Millipore, cat: 12–370) and protein complexes were immunoprecipitated using protein A dynabeads (Thermo Fischer, cat: 10001D) in IP low buffer (Active Motif). Washed beads were washed, resuspended in 5X SDS-PAGE sample buffer, and incubated at 95 degrees for 5 minutes. Proteins were separated by SDS-PAGE and transferred to a PVDF membrane for western blot detection using a monoclonal antibody against T-bet (Santa Cruz, cat: sc-21749x).
van der Veeken J., Zhong Y., Sharma R., Mazutis L., Dao P., Pe’er D., Leslie C.S, & Rudensky A.Y. (2019). Natural genetic variation reveals key features of epigenetic and transcriptional memory in virus-specific CD8 T cells. Immunity, 50(5), 1202-1217.e7.
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3

Investigating IGF2BP2-CDK6 mRNA Interaction

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The relationship between IGF2BP2 protein and CDK6 mRNA was determined using a Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Merck Millipore, Billerica, USA). The antibodies used for the RIP assay included anti-IGF2BP2 and control IgG (Merck Millipore). The coprecipitated RNAs were used for cDNA synthesis and evaluated by qRT-PCR.
Tang X., Tang Q., Li S., Li M, & Yang T. (2023). IGF2BP2 acts as a m6A modification regulator in laryngeal squamous cell carcinoma through facilitating CDK6 mRNA stabilization. Cell Death Discovery, 9, 371.
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4

Ago2-Mediated lncRNA and miRNA Profiling

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RIP was performed by using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Bedford, MA). Briefly, cultured Y79 and WERI-Rb1 cells were collected and resuspended in RIP lysis buffer (Solarbio), and the cell extracts were incubated with RIP buffer containing magnetic beads conjugated with human anti-Ago2 antibody (Millipore) or control IgG (Merck Millipore) at room temperature overnight. Subsequently, the magnetic beads were incubated with proteinase K after washing three times. Total RNA was subsequently isolated from the extracts using TRIzol. The relative enrichments of lncRNA RHPN1-AS1 and miR-3133 were determined by qRT-PCR analysis.
Li Z.N., Ge M.X., Cao L.J, & Yuan Z.F. (2020). lncRNA RHPN1-AS1 Serves as a Sponge for miR-3133 Modulating the Cell Proliferation of Retinoblastoma through JAK2. BioMed Research International, 2020, 3502981.
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5

Quantifying Anti-Infliximab Antibodies

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IFX (Remicade) was purchased from MSD Italia S.r.l. (Rome, Italy). The anti-IFX antibody HCA-216 was purchased from Bio-Rad Laboratories (Segrate, Italy). Control IgG was purchased from Merck Life Science S.r.l. (Rom, Italy), and 10 × Dulbecco-PBS was obtained from Euroclone S.p.A. (Pero, Italy). Tween 20 and α-lipoic acid were obtained from Sigma-Aldrich (Milan, Italy). Water was provided in-house by a Milli-Q system (Millipore, Bedford, MA, USA).
Anonymized human serum samples from blood donors were obtained from Mario Negri Biobank ‘Saturne’ Certified ISO 9001:2015, a non-profit service unit aimed at the collection and conservation of human blood samples for scientific research purposes in accordance with the Helsinki Declaration and the Italian legislation (Ministerial Decree of 15 July 1997 and subsequent updates).
Zeni L., Perri C., Cennamo N., Arcadio F., D’Agostino G., Salmona M., Beeg M, & Gobbi M. (2020). A portable optical-fibre-based surface plasmon resonance biosensor for the detection of therapeutic antibodies in human serum. Scientific Reports, 10, 11154.
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6

RNA-binding Protein Immunoprecipitation Assay

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The RNA-binding protein immunoprecipitation assay was performed using a Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore) according to the manufacturer’s instructions. Briefly, 70% confluent mouse articular chondrocytes were infected with Ad-ZFP36L1 and Ad-HSPA1A at the indicated MOIs for 24 h. Cell lysates were immunoprecipitated with anti-ZFP36L1 (3 μg, 101AP; FabGennix International) or control IgG (1 μg, CS200621; EMD Millipore Corp.) antibody. RNA was extracted from immobilized magnetic bead-bound complexes. The immunoprecipitated RNA was reverse transcribed, and the resulting cDNA was PCR-amplified. qRT-PCR reactions were performed using an iCycler thermal cycler (Bio-Rad) and SYBR premixExTaq (TaKaRa Bio). The primers for the RIP assay were designed to amplify three different ARE-containing regions of the Hspa1a 3′-UTR region (Supplementary Table 4). For each target gene, the transcript levels were normalized that of GAPDH and expressed as a fold-change relative to the indicated control.
Son Y.O., Kim H.E., Choi W.S., Chun C.H, & Chun J.S. (2019). RNA-binding protein ZFP36L1 regulates osteoarthritis by modulating members of the heat shock protein 70 family. Nature Communications, 10, 77.
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7

Investigating lncRNA-miRNA Interactions

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The relationship between lncRNA AC005224.4 and miR-140-3p was determined using a Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Merck Millipore, Billerica, MA, USA). Antibodies used for RIP assay included anti-AGO2 and control IgG (Merck Millipore). The co-precipitated RNAs were used for cDNA synthesis and evaluated by qRT-PCR.
Xiong T., Wang Y., Zhang Y., Yuan J., Zhu C, & Jiang W. (2023). lncRNA AC005224.4/miR-140-3p/SNAI2 regulating axis facilitates the invasion and metastasis of ovarian cancer through epithelial-mesenchymal transition. Chinese Medical Journal, 136(9), 1098-1110.
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8

Chromatin Immunoprecipitation Assay Protocol

Cited 1 time
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Chromatin immunoprecipitation (ChIP) assays were performed as previously described (24 (link), 35 (link)). Chromatin was prepared from RAW 264.7 cells treated with LPS for 4 hours and crosslinked in 1% formaldehyde for 10 minutes. Chromatin was sonicated to an average length of 400–600bp. 5 µg of chromatin was immunoprecipitated with Protein A beads using 0.5µg of antibodies for control IgG (rabbit polyclonal antibody, EMD Millipore, Billerica, MA), NF-κB p65 (clone sc-372, Santa Cruz Biotechnology Inc., Santa Cruz, CA), H3K4me1 (rabbit polyclonal antibody, EMD Millipore, Billerica, MA), H3K4me3 (rabbit polyclonal antibody, EMD Millipore, Billerica, MA), or H3K27ac (rabbit polyclonal antibody, EMD Millipore, Billerica, MA). Immunoprecipitates were then quantitated by quantitative real-time PCR and calculated as a percent of input. Primer sets used in these assays are provided in Supplemental Table S1. All ChIP assays were performed at least three times from independent experiments. The data were averaged and plotted as percent of input chromatin.
Bally A.P., Lu P., Tang Y., Austin J.W., Scharer C.D., Ahmed R, & Boss J.M. (2015). NF-κB regulates PD-1 expression in macrophages. Journal of immunology (Baltimore, Md. : 1950), 194(9), 4545-4554.
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9

Immunoprecipitation of Polycomb and LSD1

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H1299 and H1975 cells were lysed for immunoprecipitation of endogenous polycomb repressive complex2 and LSD1. The supernatants were incubated with protein A/G Sepharose beads coated with antibodies that recognized EZH2, LSD1, SNRNP70 or with control IgG (EMD Millipore, Darmstadt, Germany) for 6 h at 4 °C. After the beads were washed with wash buffer, the complexes were incubated with 0.1% SDS/0.5 mg/ml Proteinase K (30 min at 55 °C) to remove proteins, respectively. The RNA isolated from the immunoprecipitation materials was further assessed by qPCR analysis.
Li W., Sun M., Zang C., Ma P., He J., Zhang M., Huang Z., Ding Y, & Shu Y. (2016). Upregulated long non-coding RNA AGAP2-AS1 represses LATS2 and KLF2 expression through interacting with EZH2 and LSD1 in non-small-cell lung cancer cells. Cell Death & Disease, 7(5), e2225-.
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10

Investigating EZH2-PCAT6 Interaction

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In order to assess the interaction between EZH2 and PCAT6, the EZMagna RIP kit (Millipore, Billerica, MA, USA) was used. A549 cells were lysed and the supernatants were incubated with protein A/G Sepharose beads coated with antibodies that recognized EZH2 or with control IgG (EMD Millipore, Darmstadt, Germany). The antibodies were conjugated at 4 °C for 6 h. We used wash buffer to wash the beads and used 0.1% SDS/0.5 mg/ml Proteinase K to digest the proteins (30 min at 55 °C). Then, the purified RNA from the immunoprecipitation materials was used for further assessed by qPCR analysis.
Shi X., Liu Z., Liu Z., Feng X., Hua F., Hu X., Wang B., Lu K, & Nie F. (2018). Long noncoding RNA PCAT6 functions as an oncogene by binding to EZH2 and suppressing LATS2 in non-small-cell lung cancer. EBioMedicine, 37, 177-187.
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